Mineralization of matrix vesicles isolated from a human osteosarcoma cell line in culture with water-soluble chitosan-containing medium

Shizuka Yamada, Naoko Ohara, Yoshihiko Hayashi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Chitosan is a natural bioactive material. Although it has been reported that chitosan promotes osteogenesis in bone lesions, little is known about how chitosan modulates this process. The present study was designed to investigate the effect of water-soluble chitosan relative to initiation of biologic mineralization, especially in the matrix-vesicles-(MVs) mediated process in vitro. A human osteoblastic cell line (NOS-1) was used. After 3 days of incubation, the number of cells and alkaline phosphatase (ALP) activity increased significantly in the chitosan group. RT-PCR analysis revealed that chitosan induced an increase in the expression of bone morphogenetic protein-2 mRNA after 7 days of incubation. MVs were isolated from NOS-1 cells using a collagenase digestion and ultracentrifugation method. ALP activity of MVs isolated from chitosan-supplemented cells was significantly higher than that of the control group. Furthermore, isolated MVs were incubated in medium supplemented with Na-β-glycerophosphate without fetal bovine serum. Needle-like crystals were observed in association with MVs after 24 h of incubation. These needlelike crystals were densely accumulated in the chitosan group. The present findings suggest that water-soluble chitosan would promote osteoblast proliferation and differentiation and may be useful for the acceleration of initial biologic mineralization.

Original languageEnglish
Pages (from-to)500-506
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Volume66
Issue number3
Publication statusPublished - Sep 1 2003
Externally publishedYes

Fingerprint

Chitosan
Cell culture
Cells
Water
Phosphatases
Alkaline Phosphatase
Bone
Glycerophosphates
Bone Morphogenetic Protein 2
Crystals
Osteoblasts
Collagenases
Needles
Proteins
Messenger RNA

Keywords

  • Culture
  • Matrix vesicles
  • Mineralization
  • Osteosarcoma cell line
  • Water-soluble chitosan

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Mineralization of matrix vesicles isolated from a human osteosarcoma cell line in culture with water-soluble chitosan-containing medium. / Yamada, Shizuka; Ohara, Naoko; Hayashi, Yoshihiko.

In: Journal of Biomedical Materials Research - Part A, Vol. 66, No. 3, 01.09.2003, p. 500-506.

Research output: Contribution to journalArticle

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