Abstract
Mouse insulinoma (MIN6) cells were encapsulated into aminopropyl-silicate membrane deposited on calcium alginate gel beads via the sol-gel synthesis. Two alginates with different guluronic acid (G) contents, high and intermediate, but with the same molecular weights were used. Viability of the cells in the membrane templated by the alginate with an intermediate content of guluronic acid (intermediate-G) was approximately 10% higher than those in the membrane templated by the alginate with a high content of guluronic acid immediately after encapsulation. Growth of cells in vitro was hindered in case of encapsulation in the aminopropyl-silicate membrane deposited on the high-G alginate gel. The MIN6 cells in the microcapsule made from high-G alginate needed a longer period to establish a normoglycemic in recipients than those in the microcapsule made from intermediate-G alginate despite the same number of viable cells implantation. Recipients of the microcapsule with the core made from the intermediate-G alginate maintained their blood glucose values less than 300mg/dl for a longer period.
| Original language | English |
|---|---|
| Pages (from-to) | 65-73 |
| Number of pages | 9 |
| Journal | International Journal of Pharmaceutics |
| Volume | 270 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Feb 11 2004 |
| Externally published | Yes |
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Keywords
- Alginate
- Cell encapsulation
- Microcapsule
- MIN6 cell
- Silicate
- Sol-gel process
ASJC Scopus subject areas
- Pharmaceutical Science
Cite this
MIN6 cells-enclosing aminopropyl-silicate membrane templated by alginate gels differences in guluronic acid content. / Sakai, Shinji; Ono, Tsutomu; Ijima, Hiroyuki; Kawakami, Koei.
In: International Journal of Pharmaceutics, Vol. 270, No. 1-2, 11.02.2004, p. 65-73.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MIN6 cells-enclosing aminopropyl-silicate membrane templated by alginate gels differences in guluronic acid content
AU - Sakai, Shinji
AU - Ono, Tsutomu
AU - Ijima, Hiroyuki
AU - Kawakami, Koei
PY - 2004/2/11
Y1 - 2004/2/11
N2 - Mouse insulinoma (MIN6) cells were encapsulated into aminopropyl-silicate membrane deposited on calcium alginate gel beads via the sol-gel synthesis. Two alginates with different guluronic acid (G) contents, high and intermediate, but with the same molecular weights were used. Viability of the cells in the membrane templated by the alginate with an intermediate content of guluronic acid (intermediate-G) was approximately 10% higher than those in the membrane templated by the alginate with a high content of guluronic acid immediately after encapsulation. Growth of cells in vitro was hindered in case of encapsulation in the aminopropyl-silicate membrane deposited on the high-G alginate gel. The MIN6 cells in the microcapsule made from high-G alginate needed a longer period to establish a normoglycemic in recipients than those in the microcapsule made from intermediate-G alginate despite the same number of viable cells implantation. Recipients of the microcapsule with the core made from the intermediate-G alginate maintained their blood glucose values less than 300mg/dl for a longer period.
AB - Mouse insulinoma (MIN6) cells were encapsulated into aminopropyl-silicate membrane deposited on calcium alginate gel beads via the sol-gel synthesis. Two alginates with different guluronic acid (G) contents, high and intermediate, but with the same molecular weights were used. Viability of the cells in the membrane templated by the alginate with an intermediate content of guluronic acid (intermediate-G) was approximately 10% higher than those in the membrane templated by the alginate with a high content of guluronic acid immediately after encapsulation. Growth of cells in vitro was hindered in case of encapsulation in the aminopropyl-silicate membrane deposited on the high-G alginate gel. The MIN6 cells in the microcapsule made from high-G alginate needed a longer period to establish a normoglycemic in recipients than those in the microcapsule made from intermediate-G alginate despite the same number of viable cells implantation. Recipients of the microcapsule with the core made from the intermediate-G alginate maintained their blood glucose values less than 300mg/dl for a longer period.
KW - Alginate
KW - Cell encapsulation
KW - Microcapsule
KW - MIN6 cell
KW - Silicate
KW - Sol-gel process
UR - http://www.scopus.com/inward/record.url?scp=0347022246&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347022246&partnerID=8YFLogxK
U2 - 10.1016/j.ijpharm.2003.10.014
DO - 10.1016/j.ijpharm.2003.10.014
M3 - Article
C2 - 14726123
AN - SCOPUS:0347022246
VL - 270
SP - 65
EP - 73
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
SN - 0378-5173
IS - 1-2
ER -