MIN6 cells-enclosing aminopropyl-silicate membrane templated by alginate gels differences in guluronic acid content

Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Mouse insulinoma (MIN6) cells were encapsulated into aminopropyl-silicate membrane deposited on calcium alginate gel beads via the sol-gel synthesis. Two alginates with different guluronic acid (G) contents, high and intermediate, but with the same molecular weights were used. Viability of the cells in the membrane templated by the alginate with an intermediate content of guluronic acid (intermediate-G) was approximately 10% higher than those in the membrane templated by the alginate with a high content of guluronic acid immediately after encapsulation. Growth of cells in vitro was hindered in case of encapsulation in the aminopropyl-silicate membrane deposited on the high-G alginate gel. The MIN6 cells in the microcapsule made from high-G alginate needed a longer period to establish a normoglycemic in recipients than those in the microcapsule made from intermediate-G alginate despite the same number of viable cells implantation. Recipients of the microcapsule with the core made from the intermediate-G alginate maintained their blood glucose values less than 300mg/dl for a longer period.

Original languageEnglish
Pages (from-to)65-73
Number of pages9
JournalInternational Journal of Pharmaceutics
Volume270
Issue number1-2
DOIs
Publication statusPublished - Feb 11 2004
Externally publishedYes

Fingerprint

Silicates
Gels
Membranes
Capsules
Alginates
Insulinoma
Polymethyl Methacrylate
guluronic acid
alginic acid
Blood Glucose
Cell Count
Molecular Weight
Cell Membrane
Growth

Keywords

  • Alginate
  • Cell encapsulation
  • Microcapsule
  • MIN6 cell
  • Silicate
  • Sol-gel process

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

MIN6 cells-enclosing aminopropyl-silicate membrane templated by alginate gels differences in guluronic acid content. / Sakai, Shinji; Ono, Tsutomu; Ijima, Hiroyuki; Kawakami, Koei.

In: International Journal of Pharmaceutics, Vol. 270, No. 1-2, 11.02.2004, p. 65-73.

Research output: Contribution to journalArticle

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