Milk basic protein increases ghrelin secretion and bone mineral density in rodents

Yuko Ishida, Rakhi Chacrabati, Aiko Ono-Ohmachi, Zhi Gong, Chika Ikenoya, Sayaka Aizawa, Takayuki Y. Nara, Yoshikazu Morita, Ken Kato, Takafumi Sakai, Ichiro Sakata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives Milk basic protein (MBP), a mixture of proteins isolated from bovine milk, is known to increase bone formation. Ghrelin, a stomach-derived peptide hormone, also has been reported to stimulate osteoblast formation. The aim of this study was to determine whether MBP-induced bone formation is mediated via ghrelin. Methods MBP was chronically administered to mice in their drinking water for 3 wk, and body weight, water intake, and bone mineral density were measured. Additionally, plasma bone-specific alkaline phosphatase, tartrate-resistant acid phosphatase isoform 5b, and ghrelin concentrations were determined by enzyme-linked immunosorbent assay. To examine the direct effect of MBP on ghrelin secretion, gastric tissue culture and primary mucosal cells were stimulated by MBP. Results The in vivo study of young, growing mice showed that chronic MBP intake for 3 wk increased the plasma ghrelin concentration and bone mineral density of the hind limb tibia. In vitro studies using minced rat gastric mucosa tissues and primary murine isolated gastric mucosal cells revealed that MBP stimulated ghrelin release in a dose-dependent manner. Moreover, MBP-induced ghrelin secretion was partly inhibited by adrenergic blockers. Conclusions These findings suggest a novel mechanism by which MBP directly acts on ghrelin secretion. Additionally, the elevated ghrelin level induced by MBP may act as a mediator for bone formation.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalNutrition
Volume39-40
DOIs
Publication statusPublished - Jul 1 2017
Externally publishedYes

Fingerprint

Ghrelin
Milk Proteins
Bone Density
Rodentia
Osteogenesis
Stomach
Adrenergic Antagonists
Primary Cell Culture
Peptide Hormones
Body Water
Gastric Mucosa
Osteoblasts
Tibia
Drinking Water
Drinking
Alkaline Phosphatase
Protein Isoforms
Milk
Extremities
Enzyme-Linked Immunosorbent Assay

Keywords

  • Bone mineral density
  • Ghrelin
  • Milk basic protein
  • Secretion
  • β-adrenergic receptor

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Ishida, Y., Chacrabati, R., Ono-Ohmachi, A., Gong, Z., Ikenoya, C., Aizawa, S., ... Sakata, I. (2017). Milk basic protein increases ghrelin secretion and bone mineral density in rodents. Nutrition, 39-40, 15-19. https://doi.org/10.1016/j.nut.2017.02.003

Milk basic protein increases ghrelin secretion and bone mineral density in rodents. / Ishida, Yuko; Chacrabati, Rakhi; Ono-Ohmachi, Aiko; Gong, Zhi; Ikenoya, Chika; Aizawa, Sayaka; Nara, Takayuki Y.; Morita, Yoshikazu; Kato, Ken; Sakai, Takafumi; Sakata, Ichiro.

In: Nutrition, Vol. 39-40, 01.07.2017, p. 15-19.

Research output: Contribution to journalArticle

Ishida, Y, Chacrabati, R, Ono-Ohmachi, A, Gong, Z, Ikenoya, C, Aizawa, S, Nara, TY, Morita, Y, Kato, K, Sakai, T & Sakata, I 2017, 'Milk basic protein increases ghrelin secretion and bone mineral density in rodents', Nutrition, vol. 39-40, pp. 15-19. https://doi.org/10.1016/j.nut.2017.02.003
Ishida, Yuko ; Chacrabati, Rakhi ; Ono-Ohmachi, Aiko ; Gong, Zhi ; Ikenoya, Chika ; Aizawa, Sayaka ; Nara, Takayuki Y. ; Morita, Yoshikazu ; Kato, Ken ; Sakai, Takafumi ; Sakata, Ichiro. / Milk basic protein increases ghrelin secretion and bone mineral density in rodents. In: Nutrition. 2017 ; Vol. 39-40. pp. 15-19.
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abstract = "Objectives Milk basic protein (MBP), a mixture of proteins isolated from bovine milk, is known to increase bone formation. Ghrelin, a stomach-derived peptide hormone, also has been reported to stimulate osteoblast formation. The aim of this study was to determine whether MBP-induced bone formation is mediated via ghrelin. Methods MBP was chronically administered to mice in their drinking water for 3 wk, and body weight, water intake, and bone mineral density were measured. Additionally, plasma bone-specific alkaline phosphatase, tartrate-resistant acid phosphatase isoform 5b, and ghrelin concentrations were determined by enzyme-linked immunosorbent assay. To examine the direct effect of MBP on ghrelin secretion, gastric tissue culture and primary mucosal cells were stimulated by MBP. Results The in vivo study of young, growing mice showed that chronic MBP intake for 3 wk increased the plasma ghrelin concentration and bone mineral density of the hind limb tibia. In vitro studies using minced rat gastric mucosa tissues and primary murine isolated gastric mucosal cells revealed that MBP stimulated ghrelin release in a dose-dependent manner. Moreover, MBP-induced ghrelin secretion was partly inhibited by adrenergic blockers. Conclusions These findings suggest a novel mechanism by which MBP directly acts on ghrelin secretion. Additionally, the elevated ghrelin level induced by MBP may act as a mediator for bone formation.",
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AU - Aizawa, Sayaka

AU - Nara, Takayuki Y.

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