Migration of biological control agent Rhizobium vitis strain ARK-1 in grapevine stems and inhibition of galls caused by tumorigenic strain of R. vitis

Akira Kawaguchi; Yoshiteru Noutoshi

doi:10.1007/s10327-021-01043-4

Migration of biological control agent Rhizobium vitis strain ARK-1 in grapevine stems and inhibition of galls caused by tumorigenic strain of R. vitis

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Abstract

Nonpathogenic strain ARK-1 of Rhizobium vitis has antagonistic activity against a tumorigenic (Ti) strain of R. vitis and works as a biocontrol agent, but the temporal dynamics of the bacterial colonization in plants needs to be understood. Here we showed that the incidence of galls induced by Ti strain was reduced to about 60% when both upper and lower positions on the grapevine stem were inoculated with ARK-1 3 cm from the site that was inoculated with Ti 5 days later. A bacterial cell detection assay in the grapevine tissue revealed that ARK-1 moved at least 3 cm in 5 days.

Original language	English
Journal	Journal of General Plant Pathology
DOIs	https://doi.org/10.1007/s10327-021-01043-4
Publication status	Accepted/In press - 2021

Keywords

Biological control
Grapevine crown gall
Inhibiting gall formation
Migration through grapevine
Rhizobium vitis

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s10327-021-01043-4

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title = "Migration of biological control agent Rhizobium vitis strain ARK-1 in grapevine stems and inhibition of galls caused by tumorigenic strain of R. vitis",

abstract = "Nonpathogenic strain ARK-1 of Rhizobium vitis has antagonistic activity against a tumorigenic (Ti) strain of R. vitis and works as a biocontrol agent, but the temporal dynamics of the bacterial colonization in plants needs to be understood. Here we showed that the incidence of galls induced by Ti strain was reduced to about 60% when both upper and lower positions on the grapevine stem were inoculated with ARK-1 3 cm from the site that was inoculated with Ti 5 days later. A bacterial cell detection assay in the grapevine tissue revealed that ARK-1 moved at least 3 cm in 5 days.",

keywords = "Biological control, Grapevine crown gall, Inhibiting gall formation, Migration through grapevine, Rhizobium vitis",

author = "Akira Kawaguchi and Yoshiteru Noutoshi",

note = "Funding Information: This research was supported by KAKENHI Grant 17H03778 and 20K20572 from the Ministry of Education, Culture, Sports, Science and Technology of Japan to A. Kawaguchi and Y. Noutoshi. We are grateful to Dr. K. Inoue (Research Institute for Agriculture, Okayama Prefectural Technology Center for Agriculture, Forestry and Fisheries) who gave us much useful advice. Publisher Copyright: {\textcopyright} 2021, The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature.",

year = "2021",

doi = "10.1007/s10327-021-01043-4",

language = "English",

journal = "Journal of General Plant Pathology",

issn = "1345-2630",

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T1 - Migration of biological control agent Rhizobium vitis strain ARK-1 in grapevine stems and inhibition of galls caused by tumorigenic strain of R. vitis

AU - Kawaguchi, Akira

AU - Noutoshi, Yoshiteru

N1 - Funding Information: This research was supported by KAKENHI Grant 17H03778 and 20K20572 from the Ministry of Education, Culture, Sports, Science and Technology of Japan to A. Kawaguchi and Y. Noutoshi. We are grateful to Dr. K. Inoue (Research Institute for Agriculture, Okayama Prefectural Technology Center for Agriculture, Forestry and Fisheries) who gave us much useful advice. Publisher Copyright: © 2021, The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature.

PY - 2021

Y1 - 2021

N2 - Nonpathogenic strain ARK-1 of Rhizobium vitis has antagonistic activity against a tumorigenic (Ti) strain of R. vitis and works as a biocontrol agent, but the temporal dynamics of the bacterial colonization in plants needs to be understood. Here we showed that the incidence of galls induced by Ti strain was reduced to about 60% when both upper and lower positions on the grapevine stem were inoculated with ARK-1 3 cm from the site that was inoculated with Ti 5 days later. A bacterial cell detection assay in the grapevine tissue revealed that ARK-1 moved at least 3 cm in 5 days.

AB - Nonpathogenic strain ARK-1 of Rhizobium vitis has antagonistic activity against a tumorigenic (Ti) strain of R. vitis and works as a biocontrol agent, but the temporal dynamics of the bacterial colonization in plants needs to be understood. Here we showed that the incidence of galls induced by Ti strain was reduced to about 60% when both upper and lower positions on the grapevine stem were inoculated with ARK-1 3 cm from the site that was inoculated with Ti 5 days later. A bacterial cell detection assay in the grapevine tissue revealed that ARK-1 moved at least 3 cm in 5 days.

KW - Biological control

KW - Grapevine crown gall

KW - Inhibiting gall formation

KW - Migration through grapevine

KW - Rhizobium vitis

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U2 - 10.1007/s10327-021-01043-4

DO - 10.1007/s10327-021-01043-4

M3 - Article

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JO - Journal of General Plant Pathology

JF - Journal of General Plant Pathology

SN - 1345-2630

ER -

Migration of biological control agent Rhizobium vitis strain ARK-1 in grapevine stems and inhibition of galls caused by tumorigenic strain of R. vitis

Abstract

Keywords

ASJC Scopus subject areas

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