Migration behavior of alkylphenols, bisphenol A and bisphenol S studied by capillary electrophoresis using sulfated βcyclodextrin

Masanobu Mori, Hisako Naraoka, Hirohito Tsue, Tatsuya Morozumi, Takashi Kaneta, Shunitz Tanaka

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An application of capillary electrophoresis (CE) using sulfated β-cyclodextrin (SCD) has been investigated for separating alkylphenols with different chain lengths, as well as bisphenol A and bisphenol S. In the absence of SCD in running buffer, all the phenols migrated at the same velocity as the electroosmotic flow (EOF), whereas the addition of SCD effectively led to the baseline separation of alkylphenols on the basis of the difference in the abilities to bind into the hydrophobic cavity of CD. The host-guest binding constants between analyte phenols and SCD were evaluated from Benesi-Hildebrand plots of the data obtained by two independent methods, CE and UV-visible measurements, demonstrating that the greater the hydrophobicity of the phenols, the larger the binding constants. The effects of organic solvents on the resolution for alkylphenols and bisphenols were also examined. This system using SCD was effective for the separation of 4-octylphenol and 4-nonylphenol isomers having longer alkyl chains.

Original languageEnglish
Pages (from-to)763-768
Number of pages6
JournalAnalytical Sciences
Issue number6
Publication statusPublished - 2001
Externally publishedYes


ASJC Scopus subject areas

  • Analytical Chemistry

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