Migration and differentiation of GFP-transplanted bone marrow-derived cells into experimentally induced periodontal polyp in Mice

Saeka Matsuda, Masahito Shoumura, Naoto Osuga, Hidetsugu Tsujigiwa, Keisuke Nakano, Norimasa Okafuji, Takanaga Ochiai, Hiromasa Hasegawa, Toshiyuki Kawakami

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Perforation of floor of the dental pulp is often encountered during root canal treatment in routine clinical practice of dental caries. If perforation were large, granulation tissue would grow to form periodontal polyp. Granulation tissue consists of proliferating cells however their origin is not clear. It was shown that the cells in granulation tissue are mainly from migration of undifferentiated mesenchymal cells of the bone marrow. Hence, this study utilized GFP bone marrow transplantation mouse model. The floor of the pulp chamber in maxillary first molar was perforated using ½ dental round bur. Morphological assessment was carried out by micro CT and microscopy and GFP cell mechanism was further assessed by immunohistochemistry using double fluorescent staining with GFP-S100A4; GFP-Runx2 and GFP-CD31. Results of micro CT revealed alveolar bone resorption and widening of periodontal ligament. Histopathological examination showed proliferation of fibroblasts with some round cells and blood vessels in the granulation tissue. At 2 weeks, the outermost layer of the granulation tissue was lined by squamous cells with distinct intercellular bridges. At 4 weeks, the granulation tissue became larger than the perforation and the outermost layer was lined by relatively typical stratified squamous epithelium. Double immunofluorescent staining of GFP and Runx2 revealed that both proteins were expressed in spindle-shaped cells. Double immunofluorescent staining of GFP and CD31 revealed that both proteins were expressed in vascular endothelial cells in morphologically distinct vessels. The results suggest that fibroblasts, periodontal ligament fibroblasts and blood vessels in granulation tissue were derived from transplanted-bone marrow cells. Thus, essential growth of granulation tissue in periodontal polyp was caused by the migration of undifferentiated mesenchymal cells derived from bone marrow, which differentiated into fibroblasts and later on differentiated into other cells in response to injury.

Original languageEnglish
Pages (from-to)500-506
Number of pages7
JournalInternational Journal of Medical Sciences
Volume13
Issue number7
DOIs
Publication statusPublished - Jun 29 2016

Fingerprint

Granulation Tissue
Polyps
Bone Marrow Cells
Fibroblasts
Periodontal Ligament
Dental Pulp Cavity
Staining and Labeling
Blood Vessels
Alveolar Bone Loss
Dental Pulp
Dental Caries
Bone Resorption
Bone Marrow Transplantation
Microscopy
Tooth
Proteins
Epithelium
Endothelial Cells
Epithelial Cells
Immunohistochemistry

Keywords

  • Cell differentiation
  • Immunohistochemistry
  • Periodontal ligament
  • Periodontal polyp

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Migration and differentiation of GFP-transplanted bone marrow-derived cells into experimentally induced periodontal polyp in Mice. / Matsuda, Saeka; Shoumura, Masahito; Osuga, Naoto; Tsujigiwa, Hidetsugu; Nakano, Keisuke; Okafuji, Norimasa; Ochiai, Takanaga; Hasegawa, Hiromasa; Kawakami, Toshiyuki.

In: International Journal of Medical Sciences, Vol. 13, No. 7, 29.06.2016, p. 500-506.

Research output: Contribution to journalArticle

Matsuda, Saeka ; Shoumura, Masahito ; Osuga, Naoto ; Tsujigiwa, Hidetsugu ; Nakano, Keisuke ; Okafuji, Norimasa ; Ochiai, Takanaga ; Hasegawa, Hiromasa ; Kawakami, Toshiyuki. / Migration and differentiation of GFP-transplanted bone marrow-derived cells into experimentally induced periodontal polyp in Mice. In: International Journal of Medical Sciences. 2016 ; Vol. 13, No. 7. pp. 500-506.
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T1 - Migration and differentiation of GFP-transplanted bone marrow-derived cells into experimentally induced periodontal polyp in Mice

AU - Matsuda, Saeka

AU - Shoumura, Masahito

AU - Osuga, Naoto

AU - Tsujigiwa, Hidetsugu

AU - Nakano, Keisuke

AU - Okafuji, Norimasa

AU - Ochiai, Takanaga

AU - Hasegawa, Hiromasa

AU - Kawakami, Toshiyuki

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N2 - Perforation of floor of the dental pulp is often encountered during root canal treatment in routine clinical practice of dental caries. If perforation were large, granulation tissue would grow to form periodontal polyp. Granulation tissue consists of proliferating cells however their origin is not clear. It was shown that the cells in granulation tissue are mainly from migration of undifferentiated mesenchymal cells of the bone marrow. Hence, this study utilized GFP bone marrow transplantation mouse model. The floor of the pulp chamber in maxillary first molar was perforated using ½ dental round bur. Morphological assessment was carried out by micro CT and microscopy and GFP cell mechanism was further assessed by immunohistochemistry using double fluorescent staining with GFP-S100A4; GFP-Runx2 and GFP-CD31. Results of micro CT revealed alveolar bone resorption and widening of periodontal ligament. Histopathological examination showed proliferation of fibroblasts with some round cells and blood vessels in the granulation tissue. At 2 weeks, the outermost layer of the granulation tissue was lined by squamous cells with distinct intercellular bridges. At 4 weeks, the granulation tissue became larger than the perforation and the outermost layer was lined by relatively typical stratified squamous epithelium. Double immunofluorescent staining of GFP and Runx2 revealed that both proteins were expressed in spindle-shaped cells. Double immunofluorescent staining of GFP and CD31 revealed that both proteins were expressed in vascular endothelial cells in morphologically distinct vessels. The results suggest that fibroblasts, periodontal ligament fibroblasts and blood vessels in granulation tissue were derived from transplanted-bone marrow cells. Thus, essential growth of granulation tissue in periodontal polyp was caused by the migration of undifferentiated mesenchymal cells derived from bone marrow, which differentiated into fibroblasts and later on differentiated into other cells in response to injury.

AB - Perforation of floor of the dental pulp is often encountered during root canal treatment in routine clinical practice of dental caries. If perforation were large, granulation tissue would grow to form periodontal polyp. Granulation tissue consists of proliferating cells however their origin is not clear. It was shown that the cells in granulation tissue are mainly from migration of undifferentiated mesenchymal cells of the bone marrow. Hence, this study utilized GFP bone marrow transplantation mouse model. The floor of the pulp chamber in maxillary first molar was perforated using ½ dental round bur. Morphological assessment was carried out by micro CT and microscopy and GFP cell mechanism was further assessed by immunohistochemistry using double fluorescent staining with GFP-S100A4; GFP-Runx2 and GFP-CD31. Results of micro CT revealed alveolar bone resorption and widening of periodontal ligament. Histopathological examination showed proliferation of fibroblasts with some round cells and blood vessels in the granulation tissue. At 2 weeks, the outermost layer of the granulation tissue was lined by squamous cells with distinct intercellular bridges. At 4 weeks, the granulation tissue became larger than the perforation and the outermost layer was lined by relatively typical stratified squamous epithelium. Double immunofluorescent staining of GFP and Runx2 revealed that both proteins were expressed in spindle-shaped cells. Double immunofluorescent staining of GFP and CD31 revealed that both proteins were expressed in vascular endothelial cells in morphologically distinct vessels. The results suggest that fibroblasts, periodontal ligament fibroblasts and blood vessels in granulation tissue were derived from transplanted-bone marrow cells. Thus, essential growth of granulation tissue in periodontal polyp was caused by the migration of undifferentiated mesenchymal cells derived from bone marrow, which differentiated into fibroblasts and later on differentiated into other cells in response to injury.

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