Midline governs axon pathfinding by coordinating expression of two major guidance systems

Qing Xin Liu, Masaki Hiramoto, Hitoshi Ueda, Takashi Gojobori, Yasushi Hiromi, Susumu Hirose

Research output: Contribution to journalArticle

26 Citations (Scopus)


Formation of the neural network requires concerted action of multiple axon guidance systems. How neurons orchestrate expression of multiple guidance genes is poorly understood. Here, we show that Drosophila T-box protein Midline controls expression of genes encoding components of two major guidance systems: Frazzled, ROBO, and Slit. In midline mutant, expression of all these molecules are reduced, resulting in severe axon guidance defects, whereas misexpression of Midline induces their expression. Midline is present on the promoter regions of these genes, indicating that Midline controls transcription directly. We propose that Midline controls axon pathfinding through coordinating the two guidance systems.

Original languageEnglish
Pages (from-to)1165-1170
Number of pages6
JournalGenes and Development
Issue number10
Publication statusPublished - May 15 2009



  • Axon guidance
  • Frazzled
  • Midline
  • Robo
  • Slit

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Medicine(all)

Cite this