Microvesicles isolated from bovine pineal gland specifically accumulate l-glutamate

Yoshinori Moriyama, Akitsugu Yamamoto

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Pinealocytes, endocrine cells that synthesize and secrete melatonin, possess a large number of synaptic-like microvesicles (MVs) containing synaptophysin. By monitoring cross-reactivity with anti-synaptophysin antibody, the MVs were highly purified from bovine pineal glands. The purified MVs were morphologically similar to but distinct from neuronal synaptic vesicles by their lack of synapsin I. Immunological study indicated that the MVs contained vacuolar H+-ATPase, synaptotagmin and synaptobrevin 2 (VAMP2). The MVs accumulated l-glutamate at the expense of ATP hydrolysis by vacuolar H+-ATPase. No uptakes of melatonin, serotonin, noradrenaline, γ-aminobutyrate or acetylcholine were observed. These results indicated that the MVs are organelles for storage of l-glutamate in pinealocytes and suggested a possibility that pinealocytes transmit glutamate signals by MVs-mediated exocytosis.

Original languageEnglish
Pages (from-to)233-236
Number of pages4
JournalFEBS Letters
Volume367
Issue number3
DOIs
Publication statusPublished - Jul 3 1995
Externally publishedYes

Fingerprint

Pineal Gland
Vesicle-Associated Membrane Protein 2
Vacuolar Proton-Translocating ATPases
Glutamic Acid
Melatonin
Synaptotagmin II
Synapsins
Aminobutyrates
Synaptophysin
Endocrine Cells
Synaptic Vesicles
Exocytosis
Organelles
Acetylcholine
Anti-Idiotypic Antibodies
Hydrolysis
Serotonin
Norepinephrine
Adenosine Triphosphate
Cells

Keywords

  • Chemical transduction
  • Glutamate
  • Glutaminergic neuron
  • Microvesicle
  • Pinealocyte
  • Synaptic vesicle
  • Vacuolar H-ATPase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Microvesicles isolated from bovine pineal gland specifically accumulate l-glutamate. / Moriyama, Yoshinori; Yamamoto, Akitsugu.

In: FEBS Letters, Vol. 367, No. 3, 03.07.1995, p. 233-236.

Research output: Contribution to journalArticle

Moriyama, Yoshinori ; Yamamoto, Akitsugu. / Microvesicles isolated from bovine pineal gland specifically accumulate l-glutamate. In: FEBS Letters. 1995 ; Vol. 367, No. 3. pp. 233-236.
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