Microsatellite and CAPS markers for ethylene-related genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase genes, and their variation in melon (Cucumis melo L.)

Y. Akashi, S. Shiomi, Y. Kubo, M. Masuda, K. Kato

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To establish DNA markers for ethylene-related genes in melon (Cucumis melo L.), we searched for microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), and found two CAPSs and six microsatellite markers. Five markers were detected in two genes, CMe-ACS1 and CMe-ACO1, which were expressed during fruit ripening. A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeats (TA) was highly variable among cultivars, ranging from seven to thirty-seven. Insertion/deletion of (T)n and (A)n were also detected in CMe-ACS2. Specific CAPS and microsatellites for vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalBreeding Science
Volume51
Issue number2
DOIs
Publication statusPublished - Jan 1 2001

Keywords

  • ACC oxidase
  • ACC synthase
  • CAPS
  • Cucumis melo
  • Microsatellite
  • Phylogeny

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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