Microsatellite and CAPS markers for ethylene-related genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase genes, and their variation in melon (Cucumis melo L.)

Y. Akashi, S. Shiomi, Yasutaka Kubo, M. Masuda, Kenji Kato

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To establish DNA markers for ethylene-related genes in melon (Cucumis melo L.), we searched for microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), and found two CAPSs and six microsatellite markers. Five markers were detected in two genes, CMe-ACS1 and CMe-ACO1, which were expressed during fruit ripening. A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeats (TA) was highly variable among cultivars, ranging from seven to thirty-seven. Insertion/deletion of (T)n and (A)n were also detected in CMe-ACS2. Specific CAPS and microsatellites for vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalBreeding Science
Volume51
Issue number2
DOIs
Publication statusPublished - 2001

Fingerprint

Cucumis melo
Cucurbitaceae
aminocyclopropanecarboxylate oxidase
1-aminocyclopropane-1-carboxylic acid
melons
Microsatellite Repeats
ethylene
microsatellite repeats
Genes
genes
Far East
Genetic Markers
East Asia
Fruit
ripening
genetic polymorphism
genetic markers
fruits
1-aminocyclopropane-1-carboxylic acid oxidase
phylogeny

Keywords

  • ACC oxidase
  • ACC synthase
  • CAPS
  • Cucumis melo
  • Microsatellite
  • Phylogeny

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

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title = "Microsatellite and CAPS markers for ethylene-related genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase genes, and their variation in melon (Cucumis melo L.)",
abstract = "To establish DNA markers for ethylene-related genes in melon (Cucumis melo L.), we searched for microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), and found two CAPSs and six microsatellite markers. Five markers were detected in two genes, CMe-ACS1 and CMe-ACO1, which were expressed during fruit ripening. A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeats (TA) was highly variable among cultivars, ranging from seven to thirty-seven. Insertion/deletion of (T)n and (A)n were also detected in CMe-ACS2. Specific CAPS and microsatellites for vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.",
keywords = "ACC oxidase, ACC synthase, CAPS, Cucumis melo, Microsatellite, Phylogeny",
author = "Y. Akashi and S. Shiomi and Yasutaka Kubo and M. Masuda and Kenji Kato",
year = "2001",
doi = "10.1270/jsbbs.51.107",
language = "English",
volume = "51",
pages = "107--112",
journal = "Breeding Science",
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TY - JOUR

T1 - Microsatellite and CAPS markers for ethylene-related genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase genes, and their variation in melon (Cucumis melo L.)

AU - Akashi, Y.

AU - Shiomi, S.

AU - Kubo, Yasutaka

AU - Masuda, M.

AU - Kato, Kenji

PY - 2001

Y1 - 2001

N2 - To establish DNA markers for ethylene-related genes in melon (Cucumis melo L.), we searched for microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), and found two CAPSs and six microsatellite markers. Five markers were detected in two genes, CMe-ACS1 and CMe-ACO1, which were expressed during fruit ripening. A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeats (TA) was highly variable among cultivars, ranging from seven to thirty-seven. Insertion/deletion of (T)n and (A)n were also detected in CMe-ACS2. Specific CAPS and microsatellites for vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.

AB - To establish DNA markers for ethylene-related genes in melon (Cucumis melo L.), we searched for microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), and found two CAPSs and six microsatellite markers. Five markers were detected in two genes, CMe-ACS1 and CMe-ACO1, which were expressed during fruit ripening. A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeats (TA) was highly variable among cultivars, ranging from seven to thirty-seven. Insertion/deletion of (T)n and (A)n were also detected in CMe-ACS2. Specific CAPS and microsatellites for vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.

KW - ACC oxidase

KW - ACC synthase

KW - CAPS

KW - Cucumis melo

KW - Microsatellite

KW - Phylogeny

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