MicroRNAs in salivary exosome as potential biomarkers of aging

Tatsuya Machida; Takaaki Tomofuji; Daisuke Ekuni; Takayuki Maruyama; Toshiki Yoneda; Yuya Kawabata; Hirofumi Mizuno; Hisataka Miyai; Muneyoshi Kunitomo; Manabu Morita

doi:10.3390/ijms160921294

MicroRNAs in salivary exosome as potential biomarkers of aging

Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Takayuki Maruyama, Toshiki Yoneda, Yuya Kawabata, Hirofumi Mizuno, Hisataka Miyai, Muneyoshi Kunitomo, Manabu Morita

Research output: Contribution to journal › Article

46 Citations (Scopus)

Abstract

The aim of this study was to examine whether salivary exosomal miRNAs could be identified as aging biomarkers. Fifteen young healthy volunteers (median age, 21.0 years) and 13 old individuals (median age, 66.0 years) were recruited. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. In a microarray, 242 miRNAs were commonly detected in these two mixed samples. Based on the cut-off values of 2- or 0.5-fold changes (FC) and regulatory power for aging process, six candidate miRNAs (miR-24-3p, miR-371a-5p, miR-3175, miR-3162-5p, miR-671-5p, and miR-4667-5p) were selected. After comparing each total RNA obtained by the 15 young and 13 old individuals to validate the FC values using quantitative real-time PCR, miR-24-3p was identified as a novel candidate aging biomarker. This pilot study suggested that salivary exosomal miRNAs could be identified as candidate aging biomarkers. To confirm whether miR-24-3p in salivary exosomes are suitable biomarkers of aging, further validation research is required.

Original language	English
Pages (from-to)	21294-21309
Number of pages	16
Journal	International journal of molecular sciences
Volume	16
Issue number	9
DOIs	https://doi.org/10.3390/ijms160921294
Publication status	Published - Sep 7 2015

Keywords

Aging
Exosome
MicroRNA
Saliva

ASJC Scopus subject areas

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

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Machida, T., Tomofuji, T., Ekuni, D., Maruyama, T., Yoneda, T., Kawabata, Y., Mizuno, H., Miyai, H., Kunitomo, M., & Morita, M. (2015). MicroRNAs in salivary exosome as potential biomarkers of aging. International journal of molecular sciences, 16(9), 21294-21309. https://doi.org/10.3390/ijms160921294

MicroRNAs in salivary exosome as potential biomarkers of aging. / Machida, Tatsuya; Tomofuji, Takaaki; Ekuni, Daisuke ; Maruyama, Takayuki ; Yoneda, Toshiki; Kawabata, Yuya; Mizuno, Hirofumi; Miyai, Hisataka; Kunitomo, Muneyoshi; Morita, Manabu.

In: International journal of molecular sciences, Vol. 16, No. 9, 07.09.2015, p. 21294-21309.

Research output: Contribution to journal › Article

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