MicroRNAs in salivary exosome as potential biomarkers of aging

Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Takayuki Maruyama, Toshiki Yoneda, Yuya Kawabata, Hirofumi Mizuno, Hisataka Miyai, Muneyoshi Kunitomo, Manabu Morita

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The aim of this study was to examine whether salivary exosomal miRNAs could be identified as aging biomarkers. Fifteen young healthy volunteers (median age, 21.0 years) and 13 old individuals (median age, 66.0 years) were recruited. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. In a microarray, 242 miRNAs were commonly detected in these two mixed samples. Based on the cut-off values of 2- or 0.5-fold changes (FC) and regulatory power for aging process, six candidate miRNAs (miR-24-3p, miR-371a-5p, miR-3175, miR-3162-5p, miR-671-5p, and miR-4667-5p) were selected. After comparing each total RNA obtained by the 15 young and 13 old individuals to validate the FC values using quantitative real-time PCR, miR-24-3p was identified as a novel candidate aging biomarker. This pilot study suggested that salivary exosomal miRNAs could be identified as candidate aging biomarkers. To confirm whether miR-24-3p in salivary exosomes are suitable biomarkers of aging, further validation research is required.

Original languageEnglish
Pages (from-to)21294-21309
Number of pages16
JournalInternational Journal of Molecular Sciences
Volume16
Issue number9
DOIs
Publication statusPublished - Sep 7 2015

Fingerprint

Exosomes
biomarkers
Biomarkers
MicroRNAs
Aging of materials
RNA
saliva
Saliva
Real-Time Polymerase Chain Reaction
Microarrays
Healthy Volunteers
cut-off
Research

Keywords

  • Aging
  • Exosome
  • MicroRNA
  • Saliva

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications

Cite this

MicroRNAs in salivary exosome as potential biomarkers of aging. / Machida, Tatsuya; Tomofuji, Takaaki; Ekuni, Daisuke; Maruyama, Takayuki; Yoneda, Toshiki; Kawabata, Yuya; Mizuno, Hirofumi; Miyai, Hisataka; Kunitomo, Muneyoshi; Morita, Manabu.

In: International Journal of Molecular Sciences, Vol. 16, No. 9, 07.09.2015, p. 21294-21309.

Research output: Contribution to journalArticle

Machida, Tatsuya ; Tomofuji, Takaaki ; Ekuni, Daisuke ; Maruyama, Takayuki ; Yoneda, Toshiki ; Kawabata, Yuya ; Mizuno, Hirofumi ; Miyai, Hisataka ; Kunitomo, Muneyoshi ; Morita, Manabu. / MicroRNAs in salivary exosome as potential biomarkers of aging. In: International Journal of Molecular Sciences. 2015 ; Vol. 16, No. 9. pp. 21294-21309.
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