Abstract
The aim of this study was to examine whether salivary exosomal miRNAs could be identified as aging biomarkers. Fifteen young healthy volunteers (median age, 21.0 years) and 13 old individuals (median age, 66.0 years) were recruited. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. In a microarray, 242 miRNAs were commonly detected in these two mixed samples. Based on the cut-off values of 2- or 0.5-fold changes (FC) and regulatory power for aging process, six candidate miRNAs (miR-24-3p, miR-371a-5p, miR-3175, miR-3162-5p, miR-671-5p, and miR-4667-5p) were selected. After comparing each total RNA obtained by the 15 young and 13 old individuals to validate the FC values using quantitative real-time PCR, miR-24-3p was identified as a novel candidate aging biomarker. This pilot study suggested that salivary exosomal miRNAs could be identified as candidate aging biomarkers. To confirm whether miR-24-3p in salivary exosomes are suitable biomarkers of aging, further validation research is required.
| Original language | English |
|---|---|
| Pages (from-to) | 21294-21309 |
| Number of pages | 16 |
| Journal | International Journal of Molecular Sciences |
| Volume | 16 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 7 2015 |
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Keywords
- Aging
- Exosome
- MicroRNA
- Saliva
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Organic Chemistry
- Spectroscopy
- Inorganic Chemistry
- Catalysis
- Molecular Biology
- Computer Science Applications
Cite this
MicroRNAs in salivary exosome as potential biomarkers of aging. / Machida, Tatsuya; Tomofuji, Takaaki; Ekuni, Daisuke; Maruyama, Takayuki; Yoneda, Toshiki; Kawabata, Yuya; Mizuno, Hirofumi; Miyai, Hisataka; Kunitomo, Muneyoshi; Morita, Manabu.
In: International Journal of Molecular Sciences, Vol. 16, No. 9, 07.09.2015, p. 21294-21309.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MicroRNAs in salivary exosome as potential biomarkers of aging
AU - Machida, Tatsuya
AU - Tomofuji, Takaaki
AU - Ekuni, Daisuke
AU - Maruyama, Takayuki
AU - Yoneda, Toshiki
AU - Kawabata, Yuya
AU - Mizuno, Hirofumi
AU - Miyai, Hisataka
AU - Kunitomo, Muneyoshi
AU - Morita, Manabu
PY - 2015/9/7
Y1 - 2015/9/7
N2 - The aim of this study was to examine whether salivary exosomal miRNAs could be identified as aging biomarkers. Fifteen young healthy volunteers (median age, 21.0 years) and 13 old individuals (median age, 66.0 years) were recruited. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. In a microarray, 242 miRNAs were commonly detected in these two mixed samples. Based on the cut-off values of 2- or 0.5-fold changes (FC) and regulatory power for aging process, six candidate miRNAs (miR-24-3p, miR-371a-5p, miR-3175, miR-3162-5p, miR-671-5p, and miR-4667-5p) were selected. After comparing each total RNA obtained by the 15 young and 13 old individuals to validate the FC values using quantitative real-time PCR, miR-24-3p was identified as a novel candidate aging biomarker. This pilot study suggested that salivary exosomal miRNAs could be identified as candidate aging biomarkers. To confirm whether miR-24-3p in salivary exosomes are suitable biomarkers of aging, further validation research is required.
AB - The aim of this study was to examine whether salivary exosomal miRNAs could be identified as aging biomarkers. Fifteen young healthy volunteers (median age, 21.0 years) and 13 old individuals (median age, 66.0 years) were recruited. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. In a microarray, 242 miRNAs were commonly detected in these two mixed samples. Based on the cut-off values of 2- or 0.5-fold changes (FC) and regulatory power for aging process, six candidate miRNAs (miR-24-3p, miR-371a-5p, miR-3175, miR-3162-5p, miR-671-5p, and miR-4667-5p) were selected. After comparing each total RNA obtained by the 15 young and 13 old individuals to validate the FC values using quantitative real-time PCR, miR-24-3p was identified as a novel candidate aging biomarker. This pilot study suggested that salivary exosomal miRNAs could be identified as candidate aging biomarkers. To confirm whether miR-24-3p in salivary exosomes are suitable biomarkers of aging, further validation research is required.
KW - Aging
KW - Exosome
KW - MicroRNA
KW - Saliva
UR - http://www.scopus.com/inward/record.url?scp=84941248868&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941248868&partnerID=8YFLogxK
U2 - 10.3390/ijms160921294
DO - 10.3390/ijms160921294
M3 - Article
C2 - 26370963
AN - SCOPUS:84941248868
VL - 16
SP - 21294
EP - 21309
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 9
ER -