MicroRNA-664a-5p promotes neuronal differentiation of SH-SY5Y cells

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) belong to a class of small noncoding RNAs that play important roles in the translational regulation of gene expression. A number of miRNAs are known to act as key regulators of diverse processes such as neuronal differentiation. In this study, we have attempted to identify novel miRNAs related to neuronal differentiation via microarray analysis in the human neuronal differentiation model neuroblastoma SH-SY5Y cells. We identified 15 up-regulated and eight down-regulated miRNAs in SH-SY5Y cells treated with all-trans retinoic acid to induce differentiation. We further showed that one of the up-regulated miRNAs, miR-664a-5p, promoted neuronal differentiation of SH-SY5Y cells. These findings enhance our understanding of the miRNAs involved in the process of neurogenesis and, in particular, highlight an important role of miR-664a-5p in SH-SY5Y cell neuronal differentiation. Further studies will be required to confirm the function of miR-664-5p in neuronal development and disease and to identify its relevant target genes.

Original languageEnglish
JournalGenes to Cells
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

MicroRNAs
Small Untranslated RNA
Neurogenesis
Gene Expression Regulation
Microarray Analysis
Tretinoin
Neuroblastoma
Cell Differentiation
Genes

Keywords

  • MicroRNA
  • MiR-664a-5p
  • Neuronal differentiation
  • SH-SY5Y

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

MicroRNA-664a-5p promotes neuronal differentiation of SH-SY5Y cells. / Watanabe, Kazunori; Yamaji, Ryuhei; Ohtsuki, Takashi.

In: Genes to Cells, 01.01.2018.

Research output: Contribution to journalArticle

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