MicroRNA-351 regulates two-types of cell death, necrosis and apoptosis, induced by 5-fluoro-2'-deoxyuridine

Akira Sato, Takuya Omi, Akihiro Yamamoto, Akito Satake, Akiko Hiramoto, Mitsuko Masutani, Sei Ichi Tanuma, Yusuke Wataya, Hye-Sook Kim

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Abstract

Cell-death can be necrosis and apoptosis. We are investigating the mechanisms regulating the cell death that occurs on treatment of mouse cancer cell-line FM3A with antitumor 5- fluoro-2'-deoxyuridine (FUdR): necrosis occurs for the original clone F28-7, and apoptosis for its variant F28-7-A. Here we report that a microRNA (miR-351) regulates the cell death pattern. The miR-351 is expressed strongly in F28-7-A but only weakly in F28-7. Induction of a higher expression of miR-351 in F28-7 by transfecting an miRNA mimic into F28-7 resulted in a change of the death mode; necrosis to apoptosis. Furthermore, transfection of an miR-351 inhibitor into F28-7-A resulted in the morphology change, apoptosis to necrosis, in this death-by-FUdR. Possible mechanism involving lamin B1 in this miR-351's regulatory action is discussed.

Original languageEnglish
Article numbere0153130
JournalPLoS One
Volume11
Issue number4
DOIs
Publication statusPublished - Apr 1 2016

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sato, A., Omi, T., Yamamoto, A., Satake, A., Hiramoto, A., Masutani, M., Tanuma, S. I., Wataya, Y., & Kim, H-S. (2016). MicroRNA-351 regulates two-types of cell death, necrosis and apoptosis, induced by 5-fluoro-2'-deoxyuridine. PLoS One, 11(4), [e0153130]. https://doi.org/10.1371/journal.pone.0153130