MicroRNA-200c modulates epithelial-tomesenchymal transition (EMT) in human colorectal cancer metastasis

Keun Hur, Yuji Toiyama, Masanobu Takahashi, Francesc Balaguer, Takeshi Nagasaka, Junichi Koike, Hiromichi Hemmi, Minoru Koi, C. Richard Boland, Ajay Goel

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Abstract

Objective Distant metastasis is the major cause of cancer-related death in patients with colorectal cancer (CRC). Although the microRNA-200 (miR-200) family is a crucial inhibitor of epithelial-to-mesenchymal transition (EMT) in human cancer, the role of miR-200 members in the pathogenesis of metastatic CRC has not been investigated. Design Fifty-four pairs of primary CRC and corresponding matched liver metastasis tissue specimens were analysed for expression and methylation status of the miR-200 family members. Functional analysis of miR-200c overexpression was investigated in CRC cell lines, and cells were analysed for proliferation, invasion and migration. Expression of several miR-200c target genes (ZEB1, ETS1 and FLT1) and EMT markers (E-cadherin and vimentin) in CRC cell lines and tissue specimens was validated. Results Liver metastasis tissues showed higher expression of miR-200c (primary CRC=1.31 vs. liver metastasis=1.59; p=0.0014) and miR-141 (primary CRC=0.14 vs. liver metastasis=0.17; p=0.0234) than did primary CRCs, which was significantly associated with hypomethylation of the promoter region of these miRNAs (primary CRC=61.2% vs. liver metastasis=46.7%; p

Original languageEnglish
Pages (from-to)1315-1326
Number of pages12
JournalGut
Volume62
Issue number9
DOIs
Publication statusPublished - Sep 2013

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ASJC Scopus subject areas

  • Gastroenterology

Cite this

Hur, K., Toiyama, Y., Takahashi, M., Balaguer, F., Nagasaka, T., Koike, J., Hemmi, H., Koi, M., Boland, C. R., & Goel, A. (2013). MicroRNA-200c modulates epithelial-tomesenchymal transition (EMT) in human colorectal cancer metastasis. Gut, 62(9), 1315-1326. https://doi.org/10.1136/gutjnl-2011-301846