Abstract
MicroRNAs (miRNAs) are small non-coding RNAs emerging as important post-transcriptional gene regulators. In this study, we examined the role of miR-1, an miRNA specifically expressed in cardiac and skeletal muscle tissue, on the myogenic, osteoblastic, and adipogenic differentiation of C2C12 cells. Upon induction of myogenic differentiation, miR-1 was robustly expressed. Retrovirus-mediated overexpression of miR-1 markedly enhanced expression of muscle creatine kinase, sarcomeric myosin, and α-actinin, while the effects on myogenin and MyoD expression were modest. Formation of myotubes was significantly augmented in miR-1-overexpressing cells, indicating miR-1 expression enhanced not only myogenic differentiation but also maturation into myotubes. In contrast, osteoblastic and adipogenic differentiation was not affected by forced expression of miR-1. Thus, the muscle-specific miRNA, miR-1, plays important roles in controlling myogenic differentiation and maturation in lineage-committed cells, rather than functioning in fate determination.
| Original language | English |
|---|---|
| Pages (from-to) | 1006-1012 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 350 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Dec 1 2006 |
| Externally published | Yes |
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Keywords
- Adipocytes
- C2C12 cells
- Differentiation
- MicroRNA
- Osteoblasts
- Skeletal muscle cells
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
MicroRNA-1 facilitates skeletal myogenic differentiation without affecting osteoblastic and adipogenic differentiation. / Nakajima, Norio; Takahashi, Tomosaburo; Kitamura, Ryoji; Isodono, Koji; Asada, Satoshi; Ueyama, Tomomi; Matsubara, Hiroaki; Oh, Hidemasa.
In: Biochemical and Biophysical Research Communications, Vol. 350, No. 4, 01.12.2006, p. 1006-1012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MicroRNA-1 facilitates skeletal myogenic differentiation without affecting osteoblastic and adipogenic differentiation
AU - Nakajima, Norio
AU - Takahashi, Tomosaburo
AU - Kitamura, Ryoji
AU - Isodono, Koji
AU - Asada, Satoshi
AU - Ueyama, Tomomi
AU - Matsubara, Hiroaki
AU - Oh, Hidemasa
PY - 2006/12/1
Y1 - 2006/12/1
N2 - MicroRNAs (miRNAs) are small non-coding RNAs emerging as important post-transcriptional gene regulators. In this study, we examined the role of miR-1, an miRNA specifically expressed in cardiac and skeletal muscle tissue, on the myogenic, osteoblastic, and adipogenic differentiation of C2C12 cells. Upon induction of myogenic differentiation, miR-1 was robustly expressed. Retrovirus-mediated overexpression of miR-1 markedly enhanced expression of muscle creatine kinase, sarcomeric myosin, and α-actinin, while the effects on myogenin and MyoD expression were modest. Formation of myotubes was significantly augmented in miR-1-overexpressing cells, indicating miR-1 expression enhanced not only myogenic differentiation but also maturation into myotubes. In contrast, osteoblastic and adipogenic differentiation was not affected by forced expression of miR-1. Thus, the muscle-specific miRNA, miR-1, plays important roles in controlling myogenic differentiation and maturation in lineage-committed cells, rather than functioning in fate determination.
AB - MicroRNAs (miRNAs) are small non-coding RNAs emerging as important post-transcriptional gene regulators. In this study, we examined the role of miR-1, an miRNA specifically expressed in cardiac and skeletal muscle tissue, on the myogenic, osteoblastic, and adipogenic differentiation of C2C12 cells. Upon induction of myogenic differentiation, miR-1 was robustly expressed. Retrovirus-mediated overexpression of miR-1 markedly enhanced expression of muscle creatine kinase, sarcomeric myosin, and α-actinin, while the effects on myogenin and MyoD expression were modest. Formation of myotubes was significantly augmented in miR-1-overexpressing cells, indicating miR-1 expression enhanced not only myogenic differentiation but also maturation into myotubes. In contrast, osteoblastic and adipogenic differentiation was not affected by forced expression of miR-1. Thus, the muscle-specific miRNA, miR-1, plays important roles in controlling myogenic differentiation and maturation in lineage-committed cells, rather than functioning in fate determination.
KW - Adipocytes
KW - C2C12 cells
KW - Differentiation
KW - MicroRNA
KW - Osteoblasts
KW - Skeletal muscle cells
UR - http://www.scopus.com/inward/record.url?scp=33750035175&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750035175&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.09.153
DO - 10.1016/j.bbrc.2006.09.153
M3 - Article
C2 - 17045567
AN - SCOPUS:33750035175
VL - 350
SP - 1006
EP - 1012
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -