MicroRNA-1 facilitates skeletal myogenic differentiation without affecting osteoblastic and adipogenic differentiation

Norio Nakajima, Tomosaburo Takahashi, Ryoji Kitamura, Koji Isodono, Satoshi Asada, Tomomi Ueyama, Hiroaki Matsubara, Hidemasa Oh

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs emerging as important post-transcriptional gene regulators. In this study, we examined the role of miR-1, an miRNA specifically expressed in cardiac and skeletal muscle tissue, on the myogenic, osteoblastic, and adipogenic differentiation of C2C12 cells. Upon induction of myogenic differentiation, miR-1 was robustly expressed. Retrovirus-mediated overexpression of miR-1 markedly enhanced expression of muscle creatine kinase, sarcomeric myosin, and α-actinin, while the effects on myogenin and MyoD expression were modest. Formation of myotubes was significantly augmented in miR-1-overexpressing cells, indicating miR-1 expression enhanced not only myogenic differentiation but also maturation into myotubes. In contrast, osteoblastic and adipogenic differentiation was not affected by forced expression of miR-1. Thus, the muscle-specific miRNA, miR-1, plays important roles in controlling myogenic differentiation and maturation in lineage-committed cells, rather than functioning in fate determination.

Original languageEnglish
Pages (from-to)1006-1012
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume350
Issue number4
DOIs
Publication statusPublished - Dec 1 2006
Externally publishedYes

Fingerprint

MicroRNAs
Skeletal Muscle Fibers
Muscle
MM Form Creatine Kinase
Myogenin
Actinin
Small Untranslated RNA
Muscles
Retroviridae
Myosins
Regulator Genes
Cell Differentiation
Myocardium
Skeletal Muscle
Genes
Tissue

Keywords

  • Adipocytes
  • C2C12 cells
  • Differentiation
  • MicroRNA
  • Osteoblasts
  • Skeletal muscle cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

MicroRNA-1 facilitates skeletal myogenic differentiation without affecting osteoblastic and adipogenic differentiation. / Nakajima, Norio; Takahashi, Tomosaburo; Kitamura, Ryoji; Isodono, Koji; Asada, Satoshi; Ueyama, Tomomi; Matsubara, Hiroaki; Oh, Hidemasa.

In: Biochemical and Biophysical Research Communications, Vol. 350, No. 4, 01.12.2006, p. 1006-1012.

Research output: Contribution to journalArticle

Nakajima, Norio ; Takahashi, Tomosaburo ; Kitamura, Ryoji ; Isodono, Koji ; Asada, Satoshi ; Ueyama, Tomomi ; Matsubara, Hiroaki ; Oh, Hidemasa. / MicroRNA-1 facilitates skeletal myogenic differentiation without affecting osteoblastic and adipogenic differentiation. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 350, No. 4. pp. 1006-1012.
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