Microglia release ATP by exocytosis

Yoshio Imura, Yosuke Morizawa, Ryohei Komatsu, Keisuke Shibata, Youichi Shinozaki, Hirotake Kasai, Kohji Moriishi, Yoshinori Moriyama, Schuichi Koizumi

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Microglia survey the brain environment by sensing several types of diffusible molecules, among which extracellular nucleotides released/leaked from damaged cells have central roles. Microglia sense ATP or other nucleotides by multiple P2 receptors, after which they change into several different phenotypes. However, so far, it is largely unknown whether microglia themselves release ATP and, if so, by what mechanism. Here we show that exocytosis is the mechanism by which microglia release ATP. When we stimulated microglia with ionomycin, they released ATP and the release was dependent on Ca2+, vesicular H+-ATPase, or SNAREs but independent of connexin/pannexin hemichannels. VNUT was found to be expressed in microglia and exhibited no colocalization with lysosome. We also visualized the exocytosis of ATP by a quinacrine-based fluorescent time-lapse imaging. Moreover, we found that lipopolysaccharide increased the ionomycin-induced release of ATP, which was dependent on the increase in VNUT. Taken together, our data suggested that exocytosis is the mechanism of ATP release from microglia. When activated, they would release ATP by increasing VNUT-dependent exocytotic mechanisms.

Original languageEnglish
Pages (from-to)1320-1330
Number of pages11
JournalGLIA
Volume61
Issue number8
DOIs
Publication statusPublished - Aug 2013

Fingerprint

Exocytosis
Microglia
Adenosine Triphosphate
Ionomycin
Nucleotides
Time-Lapse Imaging
SNARE Proteins
Quinacrine
Connexins
Proton-Translocating ATPases
Lysosomes
Lipopolysaccharides
Phenotype
Brain

Keywords

  • ATP
  • Exocytosis
  • Microglia
  • VNUT

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neurology

Cite this

Imura, Y., Morizawa, Y., Komatsu, R., Shibata, K., Shinozaki, Y., Kasai, H., ... Koizumi, S. (2013). Microglia release ATP by exocytosis. GLIA, 61(8), 1320-1330. https://doi.org/10.1002/glia.22517

Microglia release ATP by exocytosis. / Imura, Yoshio; Morizawa, Yosuke; Komatsu, Ryohei; Shibata, Keisuke; Shinozaki, Youichi; Kasai, Hirotake; Moriishi, Kohji; Moriyama, Yoshinori; Koizumi, Schuichi.

In: GLIA, Vol. 61, No. 8, 08.2013, p. 1320-1330.

Research output: Contribution to journalArticle

Imura, Y, Morizawa, Y, Komatsu, R, Shibata, K, Shinozaki, Y, Kasai, H, Moriishi, K, Moriyama, Y & Koizumi, S 2013, 'Microglia release ATP by exocytosis', GLIA, vol. 61, no. 8, pp. 1320-1330. https://doi.org/10.1002/glia.22517
Imura Y, Morizawa Y, Komatsu R, Shibata K, Shinozaki Y, Kasai H et al. Microglia release ATP by exocytosis. GLIA. 2013 Aug;61(8):1320-1330. https://doi.org/10.1002/glia.22517
Imura, Yoshio ; Morizawa, Yosuke ; Komatsu, Ryohei ; Shibata, Keisuke ; Shinozaki, Youichi ; Kasai, Hirotake ; Moriishi, Kohji ; Moriyama, Yoshinori ; Koizumi, Schuichi. / Microglia release ATP by exocytosis. In: GLIA. 2013 ; Vol. 61, No. 8. pp. 1320-1330.
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