TY - JOUR
T1 - Microfluidic Paper Based Analytical Devices for the Detection of Carbamate Pesticides
AU - Beshana, Sheleme
AU - Hussen, Ahmed
AU - Leta, Seyoum
AU - Kaneta, Takashi
N1 - Funding Information:
The authors would like to thank Addis Ababa University, Thematic Research Fund for supporting the project entitled “Developing Innovative Microfluidic Paper-Based Analytical Devices (μPADs): Viable solution for Environmental Monitoring in Ethiopia”. Sheleme also acknowledges North Shewa Zone Administration (Oromia) for sponsoring his PhD study.
Funding Information:
This study was supported by Addis Ababa University (Grant No. VPRTT/PY-041/2018 to AH).
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Microfluidic paper-based analytical devices (μ-PADs) are a new technology platform for the development of extremely low-cost sensing applications. In this study, μ-PADs has been developed for quantitative determination of carbamate pesticides. Key experimental parameters including concentration and volume of acetylcholinesterase, acetylthiocholine iodide and 5,5′-dithiobis-(2-nitrobenzoic acid), incubation time and image capturing time were systematically optimized. Under optimal conditions, the method showed wide range of linearity (0.25–16 mg/L), repeatability (4%–5% RSD) and intermediate precision (7%–10% RSD). Limit of detection was observed to be 0.4, 0.24 and 0.46 mg/L for carbaryl, carbosulfan and furathiocarb, respectively. An acceptable mean recovery (87% to 94%) was observed for the three pesticides at 1 mg/L fortification level. The results reveal that the developed method requires minimal reagents, simple and is easy to handle. It can be used for the quantification of carbamate pesticides in resource limited laboratories without the need for the conventional analytical instruments.
AB - Microfluidic paper-based analytical devices (μ-PADs) are a new technology platform for the development of extremely low-cost sensing applications. In this study, μ-PADs has been developed for quantitative determination of carbamate pesticides. Key experimental parameters including concentration and volume of acetylcholinesterase, acetylthiocholine iodide and 5,5′-dithiobis-(2-nitrobenzoic acid), incubation time and image capturing time were systematically optimized. Under optimal conditions, the method showed wide range of linearity (0.25–16 mg/L), repeatability (4%–5% RSD) and intermediate precision (7%–10% RSD). Limit of detection was observed to be 0.4, 0.24 and 0.46 mg/L for carbaryl, carbosulfan and furathiocarb, respectively. An acceptable mean recovery (87% to 94%) was observed for the three pesticides at 1 mg/L fortification level. The results reveal that the developed method requires minimal reagents, simple and is easy to handle. It can be used for the quantification of carbamate pesticides in resource limited laboratories without the need for the conventional analytical instruments.
KW - Acetylcholinesterase
KW - Carbamate pesticides
KW - Color intensity
KW - Detection method
KW - Paper-based devices
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U2 - 10.1007/s00128-022-03533-3
DO - 10.1007/s00128-022-03533-3
M3 - Article
C2 - 35689692
AN - SCOPUS:85131661483
SN - 0007-4861
JO - Bulletin of Environmental Contamination and Toxicology
JF - Bulletin of Environmental Contamination and Toxicology
ER -