Microfluidic Paper Based Analytical Devices for the Detection of Carbamate Pesticides

Sheleme Beshana, Ahmed Hussen, Seyoum Leta, Takashi Kaneta

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Microfluidic paper-based analytical devices (μ-PADs) are a new technology platform for the development of extremely low-cost sensing applications. In this study, μ-PADs has been developed for quantitative determination of carbamate pesticides. Key experimental parameters including concentration and volume of acetylcholinesterase, acetylthiocholine iodide and 5,5′-dithiobis-(2-nitrobenzoic acid), incubation time and image capturing time were systematically optimized. Under optimal conditions, the method showed wide range of linearity (0.25–16 mg/L), repeatability (4%–5% RSD) and intermediate precision (7%–10% RSD). Limit of detection was observed to be 0.4, 0.24 and 0.46 mg/L for carbaryl, carbosulfan and furathiocarb, respectively. An acceptable mean recovery (87% to 94%) was observed for the three pesticides at 1 mg/L fortification level. The results reveal that the developed method requires minimal reagents, simple and is easy to handle. It can be used for the quantification of carbamate pesticides in resource limited laboratories without the need for the conventional analytical instruments.

Original languageEnglish
JournalBulletin of Environmental Contamination and Toxicology
Publication statusAccepted/In press - 2022


  • Acetylcholinesterase
  • Carbamate pesticides
  • Color intensity
  • Detection method
  • Paper-based devices

ASJC Scopus subject areas

  • Toxicology
  • Pollution
  • Health, Toxicology and Mutagenesis


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