Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation

Takaichi Watanabe; Shoji Takeuchi

doi:10.1109/MEMSYS.2016.7421728

Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This conference proceeding reports a preliminary result regarding a simple technique to fabricate core-shell tetra-arm poly(ethylene glycol) (tetra-PEG) hydrogel microbeads encapsulating insulin-secreting cells using microfluidic emulsification and interfacial coupling reaction of two kinds of tetra-PEG macromonomers at the core-shell interface in the precursor aqueous droplets. Different from conventional PEG-based microbeads formation, in situ gelation system utilizing self-diffusion of the macromonomers in confined microdroplets allowed to prepare tetra-PEG hydrogel microbeads with narrow size distribution under mild gelation condition in the absence of heating and UV irradiation. This technology can be used to generate monodisperse tetra-PEG hydrogel microbeads to encapsulate sensitive cells for regenerative medicines.

Original language	English
Title of host publication	MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	718-720
Number of pages	3
ISBN (Electronic)	9781509019731
DOIs	https://doi.org/10.1109/MEMSYS.2016.7421728
Publication status	Published - Feb 26 2016
Externally published	Yes
Event	29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, China Duration: Jan 24 2016 → Jan 28 2016

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2016-February
ISSN (Print)	1084-6999

Other

Other	29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
Country/Territory	China
City	Shanghai
Period	1/24/16 → 1/28/16

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2016.7421728

Cite this

Watanabe, T., & Takeuchi, S. (2016). Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation. In MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems (pp. 718-720). [7421728] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2016-February). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2016.7421728

Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation. / Watanabe, Takaichi; Takeuchi, Shoji.

MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2016. p. 718-720 7421728 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2016-February).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Watanabe, T & Takeuchi, S 2016, Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation. in MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems., 7421728, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2016-February, Institute of Electrical and Electronics Engineers Inc., pp. 718-720, 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016, Shanghai, China, 1/24/16. https://doi.org/10.1109/MEMSYS.2016.7421728

Watanabe T, Takeuchi S. Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation. In MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc. 2016. p. 718-720. 7421728. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2016.7421728

Watanabe, Takaichi ; Takeuchi, Shoji. / Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation. MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 718-720 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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abstract = "This conference proceeding reports a preliminary result regarding a simple technique to fabricate core-shell tetra-arm poly(ethylene glycol) (tetra-PEG) hydrogel microbeads encapsulating insulin-secreting cells using microfluidic emulsification and interfacial coupling reaction of two kinds of tetra-PEG macromonomers at the core-shell interface in the precursor aqueous droplets. Different from conventional PEG-based microbeads formation, in situ gelation system utilizing self-diffusion of the macromonomers in confined microdroplets allowed to prepare tetra-PEG hydrogel microbeads with narrow size distribution under mild gelation condition in the absence of heating and UV irradiation. This technology can be used to generate monodisperse tetra-PEG hydrogel microbeads to encapsulate sensitive cells for regenerative medicines.",

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Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation

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