Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation

Takaichi Watanabe, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This conference proceeding reports a preliminary result regarding a simple technique to fabricate core-shell tetra-arm poly(ethylene glycol) (tetra-PEG) hydrogel microbeads encapsulating insulin-secreting cells using microfluidic emulsification and interfacial coupling reaction of two kinds of tetra-PEG macromonomers at the core-shell interface in the precursor aqueous droplets. Different from conventional PEG-based microbeads formation, in situ gelation system utilizing self-diffusion of the macromonomers in confined microdroplets allowed to prepare tetra-PEG hydrogel microbeads with narrow size distribution under mild gelation condition in the absence of heating and UV irradiation. This technology can be used to generate monodisperse tetra-PEG hydrogel microbeads to encapsulate sensitive cells for regenerative medicines.

Original languageEnglish
Title of host publicationMEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages718-720
Number of pages3
Volume2016-February
ISBN (Electronic)9781509019731
DOIs
Publication statusPublished - Feb 26 2016
Externally publishedYes
Event29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, China
Duration: Jan 24 2016Jan 28 2016

Other

Other29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
CountryChina
CityShanghai
Period1/24/161/28/16

Fingerprint

Hydrogel
gelation
Encapsulation
Microfluidics
Hydrogels
Polyethylene glycols
insulin
encapsulating
cells
medicine
glycols
ethylene
Gelation
irradiation
heating
Emulsification
Insulin
Irradiation
Heating

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Watanabe, T., & Takeuchi, S. (2016). Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation. In MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems (Vol. 2016-February, pp. 718-720). [7421728] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2016.7421728

Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation. / Watanabe, Takaichi; Takeuchi, Shoji.

MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Vol. 2016-February Institute of Electrical and Electronics Engineers Inc., 2016. p. 718-720 7421728.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Watanabe, T & Takeuchi, S 2016, Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation. in MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. vol. 2016-February, 7421728, Institute of Electrical and Electronics Engineers Inc., pp. 718-720, 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016, Shanghai, China, 1/24/16. https://doi.org/10.1109/MEMSYS.2016.7421728
Watanabe T, Takeuchi S. Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation. In MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Vol. 2016-February. Institute of Electrical and Electronics Engineers Inc. 2016. p. 718-720. 7421728 https://doi.org/10.1109/MEMSYS.2016.7421728
Watanabe, Takaichi ; Takeuchi, Shoji. / Microfluidic formation of monodisperse tetra-PEG hydrogel microbeads for cell encapsulation. MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Vol. 2016-February Institute of Electrical and Electronics Engineers Inc., 2016. pp. 718-720
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