Microfluidic device for coulometric detection of organophosphate pesticides

Jin Wang, Takaaki Satake, Hiroaki Suzuki

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A microdevice for coulometric detection of organophosphate pesticides (OPs) was fabricated based on the measurement of the inhibition of an enzyme, acetylcholinesterase (AChE), by OPs. Thiocholine (TCh) produced in the enzymatic reaction of AChE with acetylthiocholine (ATCh) as a substrate was oxidized on a microelectrode array formed in a main flow channel. Volumes of plugs of necessary solutions were measured using a structure consisting of a row of rhombuses formed in an auxiliary flow channel. The plugs were merged and solution components were mixed at a T-junction formed with the main and auxiliary flow channels. A linear relationship was confirmed between the generated charge and the logarithm of the OP (malathion) concentration in a concentration range between 10 -6 and 10 -3 M with a correlation coefficient of 0.951. The lower limit of detection was 412 nM.

Original languageEnglish
Pages (from-to)591-595
Number of pages5
JournalAnalytical Sciences
Volume31
Issue number7
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • Acetylcholinesterase
  • Acetylthiocholine
  • Coulometry
  • Organophosphate pesticide

ASJC Scopus subject areas

  • Analytical Chemistry

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