Microfibril-associated glycoprotein-1 controls human ciliary zonule development In Vitro

Takahiro Fujita, Eichi Tsuruga, Kaori Yamanouchi, Yoshihiko Sawa, Hiroyuki Ishikawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The ciliary zonule in the eye also Known as Zinn's zonule, is composed of oxytalan fibers, which are bundles of microfibrils consisting mainly of fibrillin-1. However, it is still unclear which of the microfibril-associated molecules present in the ciliary zonule controls oxytalan fibers. Microfibril-associated glycoprotein-1 (MAGP-1) is the only microfibril-associated molecule identified in the human ciliary zonule. In the present study, we used siRNA against MAGP-1 in cultures of human non-pigmented ciliary epithelial cells to examine the extracellular deposition and appearance of fibrillin-1 employing Western blotting and immunofluorescence. MAGP-1 suppression led to a reduction of fibrillin-1 deposition. Immunofluorescence also confirmed that RNAi-mediated down-regulation of MAGP-1 led to suppression of fiber development. These results suggest that MAGP-1 plays a crucial role in the extracellular deposition of fibrillin-1 during formation of the human ciliary zonule.

Original languageEnglish
Pages (from-to)11-17
Number of pages7
JournalACTA HISTOCHEMICA ET CYTOCHEMICA
Volume47
Issue number1
DOIs
Publication statusPublished - Mar 4 2014
Externally publishedYes

Keywords

  • Ciliary zonule
  • Fibrillin
  • MAGP-1
  • Microfibrils
  • Oxytalan fibers

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

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