Microfibril-associated glycoprotein-1 controls human ciliary zonule development In Vitro

Takahiro Fujita, Eichi Tsuruga, Kaori Yamanouchi, Yoshihiko Sawa, Hiroyuki Ishikawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The ciliary zonule in the eye also Known as Zinn's zonule, is composed of oxytalan fibers, which are bundles of microfibrils consisting mainly of fibrillin-1. However, it is still unclear which of the microfibril-associated molecules present in the ciliary zonule controls oxytalan fibers. Microfibril-associated glycoprotein-1 (MAGP-1) is the only microfibril-associated molecule identified in the human ciliary zonule. In the present study, we used siRNA against MAGP-1 in cultures of human non-pigmented ciliary epithelial cells to examine the extracellular deposition and appearance of fibrillin-1 employing Western blotting and immunofluorescence. MAGP-1 suppression led to a reduction of fibrillin-1 deposition. Immunofluorescence also confirmed that RNAi-mediated down-regulation of MAGP-1 led to suppression of fiber development. These results suggest that MAGP-1 plays a crucial role in the extracellular deposition of fibrillin-1 during formation of the human ciliary zonule.

Original languageEnglish
Pages (from-to)11-17
Number of pages7
JournalActa Histochemica et Cytochemica
Volume47
Issue number1
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Microfibrils
Fluorescent Antibody Technique
Fibers
Molecules
RNA Interference
Small Interfering RNA
Down-Regulation
Western Blotting
Epithelial Cells
microfibrillar protein
In Vitro Techniques
Fibrillin-1
oxytalan

Keywords

  • Ciliary zonule
  • Fibrillin
  • MAGP-1
  • Microfibrils
  • Oxytalan fibers

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Physiology
  • Pathology and Forensic Medicine
  • Histology

Cite this

Microfibril-associated glycoprotein-1 controls human ciliary zonule development In Vitro. / Fujita, Takahiro; Tsuruga, Eichi; Yamanouchi, Kaori; Sawa, Yoshihiko; Ishikawa, Hiroyuki.

In: Acta Histochemica et Cytochemica, Vol. 47, No. 1, 2014, p. 11-17.

Research output: Contribution to journalArticle

Fujita, Takahiro ; Tsuruga, Eichi ; Yamanouchi, Kaori ; Sawa, Yoshihiko ; Ishikawa, Hiroyuki. / Microfibril-associated glycoprotein-1 controls human ciliary zonule development In Vitro. In: Acta Histochemica et Cytochemica. 2014 ; Vol. 47, No. 1. pp. 11-17.
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