TY - JOUR
T1 - Microbial degradation of 2,5-diketopiperazines
AU - Kanzaki, Hiroshi
AU - Kawazu, Kazuyoshi
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 1998
Y1 - 1998
N2 - Enrichment culture of soil samples with cyclo(Gly-Gly) and cyclo(Gly-l-Tyr) as a carbon and/or a nitrogen source resulted in the isolation of Arthrobacter sp. 1-3-1 and the coryneform rod bacterium, strain T-1-3-Y, as the bacteria which most highly assimilated cyclo(Gly-Gly) and cyclo(Gly-l-Tyr), respectively. Both bacteria had the enzyme activity hydrolyzing DKPs intracellularly in contrast to the extracellular cyclo(Gly-Gly) hydrolase reported previously. The cells of Arthrobacter sp. 1-3-1 degraded all diketopiperazines tested, including cyclo(Gly-Gly), cyclo(Gly-l-Ala), cyclo(Gly-l-Val), cyclo(Gly-l-Leu), cyclo(Gly-l-Ile), cyclo(Gly-l-Met), cyclo(Gly-l-Phe), cyclo(Gly-l-Tyr), cyclo(l-Ala-l-Tyr), cyclo(l-Val-l-Tyr), cyclo(l-Leu-l-Tyr), cyclo(l-Tyr-l-Tyr), cyclo(l-Phe-l-Leu), cyclo(Gly-d-Ala), and cyclo(Gly-d-Leu). On the other hand, the degradation of d-amino acid-containing DKPs or cyclo(Gly-Gly) by the cells of the strain T-1-3-Y were detected hardly or not at all, respectively. From the result that DKP hydrolase in the strain T-1-3-Y had a higher stereospecificity than that in Arthrobacter sp. 1-3-1, the diversity of DKP hydrolases were found to be in nature.
AB - Enrichment culture of soil samples with cyclo(Gly-Gly) and cyclo(Gly-l-Tyr) as a carbon and/or a nitrogen source resulted in the isolation of Arthrobacter sp. 1-3-1 and the coryneform rod bacterium, strain T-1-3-Y, as the bacteria which most highly assimilated cyclo(Gly-Gly) and cyclo(Gly-l-Tyr), respectively. Both bacteria had the enzyme activity hydrolyzing DKPs intracellularly in contrast to the extracellular cyclo(Gly-Gly) hydrolase reported previously. The cells of Arthrobacter sp. 1-3-1 degraded all diketopiperazines tested, including cyclo(Gly-Gly), cyclo(Gly-l-Ala), cyclo(Gly-l-Val), cyclo(Gly-l-Leu), cyclo(Gly-l-Ile), cyclo(Gly-l-Met), cyclo(Gly-l-Phe), cyclo(Gly-l-Tyr), cyclo(l-Ala-l-Tyr), cyclo(l-Val-l-Tyr), cyclo(l-Leu-l-Tyr), cyclo(l-Tyr-l-Tyr), cyclo(l-Phe-l-Leu), cyclo(Gly-d-Ala), and cyclo(Gly-d-Leu). On the other hand, the degradation of d-amino acid-containing DKPs or cyclo(Gly-Gly) by the cells of the strain T-1-3-Y were detected hardly or not at all, respectively. From the result that DKP hydrolase in the strain T-1-3-Y had a higher stereospecificity than that in Arthrobacter sp. 1-3-1, the diversity of DKP hydrolases were found to be in nature.
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U2 - 10.1016/S0165-3253(98)80020-5
DO - 10.1016/S0165-3253(98)80020-5
M3 - Article
AN - SCOPUS:77957053139
VL - 53
SP - 167
EP - 171
JO - Studies in Organic Chemistry
JF - Studies in Organic Chemistry
SN - 0165-3253
IS - C
ER -