Micro-FTIR spectroscopic signatures of Bacterial lipids in Proterozoic microfossils

Motoko Igisu, Yuichiro Ueno, Mie Shimojima, Satoru Nakashima, Stanley M. Awramik, Hiroyuki Ohta, Shigenori Maruyama

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Precambrian microbial fossils show carbonaceous cellular structure, which often resemble in shape and size cyanobacteria and other prokaryotes. Morphological taxonomy of these minute, simple, and more or less degraded fossils is, however, often not enough to determine their precise phylogenetic positions. Here we report the results of micro-FTIR spectroscopic analyses of well-preserved microfossils in ∼850 Ma and ∼1900 Ma stromatolites, together with those of 8 species of extant prokaryotes and 5 of eukaryotes for comparison. These Proterozoic fossils have low CH3/CH2 absorbance ratios (R3/2 <0.5) of aliphatic CH moieties, suggesting selective preservation of long, straight, aliphatic carbon chains probably derived from bacterial membrane lipids. All the observed R3/2 values of coccoids, filaments and amorphous organic matter resemble lipid fractions of extant Bacteria including cyanobacteria, but not Archaea. The results indicate that Proterozoic microfossils belong to Bacteria, which is consistent with the cyanobacterial origin inferred from morphology. Moreover, the R3/2 value of fossilized cell would reflect chemical composition of its precursor membrane lipid, thus could be a useful new tracer for distinguishing Archaea, Bacteria and possibly Eucarya for fossilized and extant microorganisms.

Original languageEnglish
Pages (from-to)19-26
Number of pages8
JournalPrecambrian Research
Volume173
Issue number1-4
DOIs
Publication statusPublished - Sep 2009
Externally publishedYes

Fingerprint

microfossil
Proterozoic
Bacteria
lipid
prokaryote
fossil
Membrane Lipids
Lipids
bacterium
cyanobacterium
membrane
eukaryote
Taxonomies
absorbance
Microorganisms
Biological materials
Precambrian
Carbon
microorganism
chemical composition

Keywords

  • Aliphatic CH moieties
  • Archaea
  • Bacteria
  • Lipid
  • Micro-FTIR
  • Prokaryotic fossil

ASJC Scopus subject areas

  • Geochemistry and Petrology
  • Geology

Cite this

Igisu, M., Ueno, Y., Shimojima, M., Nakashima, S., Awramik, S. M., Ohta, H., & Maruyama, S. (2009). Micro-FTIR spectroscopic signatures of Bacterial lipids in Proterozoic microfossils. Precambrian Research, 173(1-4), 19-26. https://doi.org/10.1016/j.precamres.2009.03.006

Micro-FTIR spectroscopic signatures of Bacterial lipids in Proterozoic microfossils. / Igisu, Motoko; Ueno, Yuichiro; Shimojima, Mie; Nakashima, Satoru; Awramik, Stanley M.; Ohta, Hiroyuki; Maruyama, Shigenori.

In: Precambrian Research, Vol. 173, No. 1-4, 09.2009, p. 19-26.

Research output: Contribution to journalArticle

Igisu, M, Ueno, Y, Shimojima, M, Nakashima, S, Awramik, SM, Ohta, H & Maruyama, S 2009, 'Micro-FTIR spectroscopic signatures of Bacterial lipids in Proterozoic microfossils', Precambrian Research, vol. 173, no. 1-4, pp. 19-26. https://doi.org/10.1016/j.precamres.2009.03.006
Igisu, Motoko ; Ueno, Yuichiro ; Shimojima, Mie ; Nakashima, Satoru ; Awramik, Stanley M. ; Ohta, Hiroyuki ; Maruyama, Shigenori. / Micro-FTIR spectroscopic signatures of Bacterial lipids in Proterozoic microfossils. In: Precambrian Research. 2009 ; Vol. 173, No. 1-4. pp. 19-26.
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