Micro-Enzyme Immunoassay of Vasoactive Intestinal Polypeptide (VIP)-like Immunoreactive Substance in Bovine Milk

Masaharu Takeyama; Katsumi Wakayama; Fusako Takayama; Kazuhiro Kondo; Nobutaka Fujii; H. Haruaki; Y. Yajima

doi:10.1248/cpb.38.960

Micro-Enzyme Immunoassay of Vasoactive Intestinal Polypeptide (VIP)-like Immunoreactive Substance in Bovine Milk

Masaharu Takeyama, Katsumi Wakayama, Fusako Takayama, Kazuhiro Kondo, Nobutaka Fujii, H. Haruaki, Y. Yajima

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

A sensitive and specific enzyme immunoassay for vasoactive intestinal polypeptide (VIP)-like immunoreactivity was developed with the use of synthetic carboxy-terminal (C-terminal) fragment (residue 11—28) of porcine VIP conjugated with β-D-galactosidase and a second antibody-coated immunoplate. Using 4-methylumbelliferyl β-D-galactopyranoside as a fluorogenic substrate, the minimum amount of VIP-like immunoreactive substance (VIP-IS) detectable by this method was O.l fmol/well (2.5 pmol/l). The level of VIP-IS in bovine foremilk was above 100pmol/l, which was more than eightfold higher than that in normal milk.

Original language	English
Pages (from-to)	960-962
Number of pages	3
Journal	Chemical and Pharmaceutical Bulletin
Volume	38
Issue number	4
DOIs	https://doi.org/10.1248/cpb.38.960
Publication status	Published - 1990
Externally published	Yes

Keywords

4-methylumbelliferyl β-D-galactopyranoside
galactosidase
highly sensitive enzyme immunoassay VIP(11—28)-linked β-D- bovine foremilk
vasoactive intestinal polypeptide (VIP)-like immunoreactive substance

ASJC Scopus subject areas

Chemistry(all)
Drug Discovery

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title = "Micro-Enzyme Immunoassay of Vasoactive Intestinal Polypeptide (VIP)-like Immunoreactive Substance in Bovine Milk",

abstract = "A sensitive and specific enzyme immunoassay for vasoactive intestinal polypeptide (VIP)-like immunoreactivity was developed with the use of synthetic carboxy-terminal (C-terminal) fragment (residue 11—28) of porcine VIP conjugated with β-D-galactosidase and a second antibody-coated immunoplate. Using 4-methylumbelliferyl β-D-galactopyranoside as a fluorogenic substrate, the minimum amount of VIP-like immunoreactive substance (VIP-IS) detectable by this method was O.l fmol/well (2.5 pmol/l). The level of VIP-IS in bovine foremilk was above 100pmol/l, which was more than eightfold higher than that in normal milk.",

keywords = "4-methylumbelliferyl β-D-galactopyranoside, galactosidase, highly sensitive enzyme immunoassay VIP(11—28)-linked β-D- bovine foremilk, vasoactive intestinal polypeptide (VIP)-like immunoreactive substance",

author = "Masaharu Takeyama and Katsumi Wakayama and Fusako Takayama and Kazuhiro Kondo and Nobutaka Fujii and H. Haruaki and Y. Yajima",

year = "1990",

doi = "10.1248/cpb.38.960",

language = "English",

volume = "38",

pages = "960--962",

journal = "Chemical and Pharmaceutical Bulletin",

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publisher = "Pharmaceutical Society of Japan",

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AU - Takeyama, Masaharu

AU - Wakayama, Katsumi

AU - Takayama, Fusako

AU - Kondo, Kazuhiro

AU - Fujii, Nobutaka

AU - Haruaki, H.

AU - Yajima, Y.

PY - 1990

Y1 - 1990

N2 - A sensitive and specific enzyme immunoassay for vasoactive intestinal polypeptide (VIP)-like immunoreactivity was developed with the use of synthetic carboxy-terminal (C-terminal) fragment (residue 11—28) of porcine VIP conjugated with β-D-galactosidase and a second antibody-coated immunoplate. Using 4-methylumbelliferyl β-D-galactopyranoside as a fluorogenic substrate, the minimum amount of VIP-like immunoreactive substance (VIP-IS) detectable by this method was O.l fmol/well (2.5 pmol/l). The level of VIP-IS in bovine foremilk was above 100pmol/l, which was more than eightfold higher than that in normal milk.

AB - A sensitive and specific enzyme immunoassay for vasoactive intestinal polypeptide (VIP)-like immunoreactivity was developed with the use of synthetic carboxy-terminal (C-terminal) fragment (residue 11—28) of porcine VIP conjugated with β-D-galactosidase and a second antibody-coated immunoplate. Using 4-methylumbelliferyl β-D-galactopyranoside as a fluorogenic substrate, the minimum amount of VIP-like immunoreactive substance (VIP-IS) detectable by this method was O.l fmol/well (2.5 pmol/l). The level of VIP-IS in bovine foremilk was above 100pmol/l, which was more than eightfold higher than that in normal milk.

KW - 4-methylumbelliferyl β-D-galactopyranoside

KW - galactosidase

KW - highly sensitive enzyme immunoassay VIP(11—28)-linked β-D- bovine foremilk

KW - vasoactive intestinal polypeptide (VIP)-like immunoreactive substance

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IS - 4

ER -

Micro-Enzyme Immunoassay of Vasoactive Intestinal Polypeptide (VIP)-like Immunoreactive Substance in Bovine Milk

Abstract

Keywords

ASJC Scopus subject areas

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