Micro-Enzyme Immunoassay of Vasoactive Intestinal Polypeptide (VIP)-like Immunoreactive Substance in Bovine Milk

Masaharu Takeyama, Katsumi Wakayama, Fusako Takayama, Kazuhiro Kondo, Nobutaka Fujii, H. Haruaki, Y. Yajima

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A sensitive and specific enzyme immunoassay for vasoactive intestinal polypeptide (VIP)-like immunoreactivity was developed with the use of synthetic carboxy-terminal (C-terminal) fragment (residue 11—28) of porcine VIP conjugated with β-D-galactosidase and a second antibody-coated immunoplate. Using 4-methylumbelliferyl β-D-galactopyranoside as a fluorogenic substrate, the minimum amount of VIP-like immunoreactive substance (VIP-IS) detectable by this method was O.l fmol/well (2.5 pmol/l). The level of VIP-IS in bovine foremilk was above 100pmol/l, which was more than eightfold higher than that in normal milk.

Original languageEnglish
Pages (from-to)960-962
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Volume38
Issue number4
DOIs
Publication statusPublished - 1990

Keywords

  • 4-methylumbelliferyl β-D-galactopyranoside
  • galactosidase
  • highly sensitive enzyme immunoassay VIP(11—28)-linked β-D- bovine foremilk
  • vasoactive intestinal polypeptide (VIP)-like immunoreactive substance

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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