Micelles of pulmonary surfactant in human amniotic fluid at term

Koji Nishijima, Ken Ichi Shukunami, Hirokazu Tsukahara, Makoto Orisaka, Jun'ichiro Miura, Fumikazu Kotsuji

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Studies using in vitro analysis have shown that the interaction between pulmonary surfactant and vernix caseosa could explain the appearance of amniotic fluid turbidity. That phenomenon is interpreted based on the "roll-up" hypothesis. We tested the roll-up hypothesis by examining the presence of micelles of pulmonary surfactant in human amniotic fluid at term. Amniotic fluid samples were collected from each of six healthy pregnant women at term and at 16 wk of gestation. These samples were stained negatively and analyzed using an electron microscope. Ultrastructures present in amniotic fluid were compared with the structure of micelles derived from suspended surfactant TA isolated from bovine lung. Surfactant TA formed spheroidal and rod-shaped micelles 10-70 nm in diameter above the critical micelle concentration. Identical micelle particles were described in human amniotic fluid at term. In addition, surfactant protein B was identified in the micelle fraction of amniotic fluid. However, no micelles were found in human amniotic fluid taken at 16 wk of gestation. Our results support the view that pulmonary surfactant could induce the detachment of vernix caseosa and increase the turbidity of the amniotic fluid.

Original languageEnglish
Pages (from-to)196-199
Number of pages4
JournalPediatric Research
Volume60
Issue number2
DOIs
Publication statusPublished - Aug 2006
Externally publishedYes

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Pulmonary Surfactants
Micelles
Amniotic Fluid
Vernix Caseosa
Pregnancy
Surface-Active Agents
Pregnant Women
Electrons
Lung

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Nishijima, K., Shukunami, K. I., Tsukahara, H., Orisaka, M., Miura, J., & Kotsuji, F. (2006). Micelles of pulmonary surfactant in human amniotic fluid at term. Pediatric Research, 60(2), 196-199. https://doi.org/10.1203/01.pdr.0000228329.90502.3a-

Micelles of pulmonary surfactant in human amniotic fluid at term. / Nishijima, Koji; Shukunami, Ken Ichi; Tsukahara, Hirokazu; Orisaka, Makoto; Miura, Jun'ichiro; Kotsuji, Fumikazu.

In: Pediatric Research, Vol. 60, No. 2, 08.2006, p. 196-199.

Research output: Contribution to journalArticle

Nishijima, K, Shukunami, KI, Tsukahara, H, Orisaka, M, Miura, J & Kotsuji, F 2006, 'Micelles of pulmonary surfactant in human amniotic fluid at term', Pediatric Research, vol. 60, no. 2, pp. 196-199. https://doi.org/10.1203/01.pdr.0000228329.90502.3a-
Nishijima, Koji ; Shukunami, Ken Ichi ; Tsukahara, Hirokazu ; Orisaka, Makoto ; Miura, Jun'ichiro ; Kotsuji, Fumikazu. / Micelles of pulmonary surfactant in human amniotic fluid at term. In: Pediatric Research. 2006 ; Vol. 60, No. 2. pp. 196-199.
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