Mice genetically lacking endothelial selectins are resistant to the lethality in septic peritonitis

Akihiro Matsukawa, Nicholas W. Lukacs, Cory M. Hogaboam, Randall N. Knibbs, Daniel C. Bullard, Steven L. Kunkel, Lloyd M. Stoolman

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Leukocyte interactions with vascular endothelium are an initial step for leukocyte entry into infectious foci where endothelial selectins may play a key role. Infiltrating leukocyte is essential for bacterial clearance, suggesting that endothelial selectins would be important in host defense against microorganisms. To address this, E-, P-, and E/P-selectin-deficient mice (E-/-, P-/-, E/P-/-) and wild-type (WT) mice underwent cecal ligation and puncture (CLP). Neither leukocyte infiltration nor bacterial load in the peritoneum was altered in E-/-, P-/-, and E/P-/- mice compared to WT mice. However, E-/-, P-/-, and E/P-/- mice were resistant to the lethality induced by CLP. At the mechanistic level, E-/-, P-/-, and E/P-/- mice did not develop renal dysfunction, a possible cause of death during sepsis. The serum level of interleukin-13 in E-/-, P-/-, and E/P-/- mice that had undergone CLP was higher than that in WT mice, whereas levels of macrophage inflammatory protein-2, KC in serum, and KC in kidney were lower than those in WT mice. These experiments demonstrate that endothelial selectin-mediated leukocyte rolling is not required for leukocyte entry in septic peritonitis and that endothelial selectins may affect mice survival during sepsis by influencing the cytokine profiles.

Original languageEnglish
Pages (from-to)68-76
Number of pages9
JournalExperimental and Molecular Pathology
Volume72
Issue number1
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Selectins
Peritonitis
Leukocytes
Chemokine CXCL2
P-Selectin
Punctures
Interleukin-13
Ligation
Infiltration
Microorganisms
Cytokines
Sepsis
Leukocyte Rolling
Kidney
E-Selectin
Bacterial Load
Peritoneum
Vascular Endothelium
Serum
Experiments

Keywords

  • Cytokine balance
  • Endothelial selectin
  • Leukocyte infiltration
  • Renal dysfunction
  • Sepsis

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine

Cite this

Matsukawa, A., Lukacs, N. W., Hogaboam, C. M., Knibbs, R. N., Bullard, D. C., Kunkel, S. L., & Stoolman, L. M. (2002). Mice genetically lacking endothelial selectins are resistant to the lethality in septic peritonitis. Experimental and Molecular Pathology, 72(1), 68-76. https://doi.org/10.1006/exmp.2001.2416

Mice genetically lacking endothelial selectins are resistant to the lethality in septic peritonitis. / Matsukawa, Akihiro; Lukacs, Nicholas W.; Hogaboam, Cory M.; Knibbs, Randall N.; Bullard, Daniel C.; Kunkel, Steven L.; Stoolman, Lloyd M.

In: Experimental and Molecular Pathology, Vol. 72, No. 1, 2002, p. 68-76.

Research output: Contribution to journalArticle

Matsukawa, Akihiro ; Lukacs, Nicholas W. ; Hogaboam, Cory M. ; Knibbs, Randall N. ; Bullard, Daniel C. ; Kunkel, Steven L. ; Stoolman, Lloyd M. / Mice genetically lacking endothelial selectins are resistant to the lethality in septic peritonitis. In: Experimental and Molecular Pathology. 2002 ; Vol. 72, No. 1. pp. 68-76.
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