Abstract
Leukocyte interactions with vascular endothelium are an initial step for leukocyte entry into infectious foci where endothelial selectins may play a key role. Infiltrating leukocyte is essential for bacterial clearance, suggesting that endothelial selectins would be important in host defense against microorganisms. To address this, E-, P-, and E/P-selectin-deficient mice (E-/-, P-/-, E/P-/-) and wild-type (WT) mice underwent cecal ligation and puncture (CLP). Neither leukocyte infiltration nor bacterial load in the peritoneum was altered in E-/-, P-/-, and E/P-/- mice compared to WT mice. However, E-/-, P-/-, and E/P-/- mice were resistant to the lethality induced by CLP. At the mechanistic level, E-/-, P-/-, and E/P-/- mice did not develop renal dysfunction, a possible cause of death during sepsis. The serum level of interleukin-13 in E-/-, P-/-, and E/P-/- mice that had undergone CLP was higher than that in WT mice, whereas levels of macrophage inflammatory protein-2, KC in serum, and KC in kidney were lower than those in WT mice. These experiments demonstrate that endothelial selectin-mediated leukocyte rolling is not required for leukocyte entry in septic peritonitis and that endothelial selectins may affect mice survival during sepsis by influencing the cytokine profiles.
Original language | English |
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Pages (from-to) | 68-76 |
Number of pages | 9 |
Journal | Experimental and Molecular Pathology |
Volume | 72 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 1 2002 |
Externally published | Yes |
Keywords
- Cytokine balance
- Endothelial selectin
- Leukocyte infiltration
- Renal dysfunction
- Sepsis
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Molecular Biology
- Clinical Biochemistry