MGST2 and WNT2 are candidate genes for comitant strabismus susceptibility in Japanese patients

Jingjing Zhang, Toshihiko Matsuo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background/Aim. Strabismus is a common condition with misalignment between two eyes that may lead to decrease of visual acuity, lack of binocularity, and diplopia. It is caused by heterogeneous environmental and genetic risk factors. Our previous research has identified new chromosomal susceptibility loci in 4q28.3 and 7q31.2 regions for comitant strabismus in Japanese families. We conducted a verification study by linkage analysis to narrow the chromosomal loci down to a single gene. Methods. From Japanese and U.S. databases, 24 rsSNPs and 233 rsSNPs were chosen from the 4q28.3 and 7q31.2 region, respectively, and were typed in 108 affected subjects and 96 unaffected subjects of 58 families with primary and non-syndromic comitant strabismus. Three major analytical methods were used: transmission disequilibrium test (TDT), TDT allowing for errors (TDTae), and linkage analysis under dominant and recessive inheritance. Results. The SNPs with significant P values inTDT andTDTae were located solely at the gene, microsomal glutathione S-transferase 2 (MGST2), on chromosome 4q28.3 locus. In contrast, significant SNPs were dispersed in a few genes, containing wingless-type MMTV integration site family member 2 (WNT2), on chromosome 7q31.2 locus. The distribution of significant SNPs on the 7q31.2 locus showed that only the ST7 to WNT2 region in the same big haplotype block contained significant SNPs for all three methods of linkage analysis. Conclusions. This study suggests that MGST2 and WNT2 are potential candidates for comitant strabismus in Japanese population.

Original languageEnglish
Article numbere3935
JournalPeerJ
Volume2017
Issue number10
DOIs
Publication statusPublished - 2017

Fingerprint

Strabismus
glutathione transferase
Single Nucleotide Polymorphism
Genes
Chromosomes
loci
linkage (genetics)
genes
Diplopia
chromosomes
Haplotypes
Visual Acuity
analytical methods
Databases
inheritance (genetics)
haplotypes
risk factors
eyes
testing
leukotriene-C4 synthase

Keywords

  • Candidate gene
  • Case-control association study
  • Chromosomal susceptibility locus
  • Comitant strabismus
  • Esotropia
  • Exotropia
  • Japanese families
  • Linkage analysis
  • Transmission disequilibrium test (TDT)
  • Transmission disequilibrium test allowing for errors (TDTae)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

MGST2 and WNT2 are candidate genes for comitant strabismus susceptibility in Japanese patients. / Zhang, Jingjing; Matsuo, Toshihiko.

In: PeerJ, Vol. 2017, No. 10, e3935, 2017.

Research output: Contribution to journalArticle

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