Methylprednisolone accelerates the resolution of glomerulonephritis by sensitizing mesangial cells to apoptosis

Keisuke Maruyama, Naoki Kashihara, Yasushi Yamasaki, Minoru Sato, Hitoshi Sugiyama, Kazunori Okamoto, Yohei Maeshima, Masahiro Odawara, Junzo Sasaki, Hirofumi Makino

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Glucocorticoid has long been used to treat patients with glomerulonephritis because it ameliorates mesangial cell proliferation and proteinuria, in part by suppressing nuclear factor-kappa B (NF-κB) activation, which regulates the transcription of various pro-inflammatory genes. Recent evidence shows that NF-κB activation increases the resistance to TNF-α-induced apoptosis in mesangial cells. We examined glomerular cell proliferation and apoptosis along with NF-κB activation in the Thy-1.1 nephritis model. We also evaluated TNF-α-induced apoptosis in cultured mesangial cells. Methylprednisolone treatment ameliorated mesangial hypercellularity in Thy-1.1 nephritis by decreasing proliferating cells and increasing apoptosis in the glomeruli. These effects were associated with suppressed NF-κB activation. This in vitro study revealed that treatment with methylprednisolone and TNF-α induced cultured mesangial cell apoptosis. These results suggest that methylprednisolone may accelerate the resolution phase of Thy-1.1 nephritis in part by sensitizing mesangial cells to apoptosis.

Original languageEnglish
Pages (from-to)317-326
Number of pages10
JournalExperimental Nephrology
Volume9
Issue number5
Publication statusPublished - 2001

Fingerprint

Mesangial Cells
Methylprednisolone
Glomerulonephritis
NF-kappa B
Apoptosis
Nephritis
Cultured Cells
Cell Proliferation
Proteinuria
Glucocorticoids
Therapeutics
Genes

Keywords

  • Apoptosis
  • Glomerulonephritis
  • Mesangial cell
  • Methylprednisolone
  • NF-κB

ASJC Scopus subject areas

  • Nephrology

Cite this

Methylprednisolone accelerates the resolution of glomerulonephritis by sensitizing mesangial cells to apoptosis. / Maruyama, Keisuke; Kashihara, Naoki; Yamasaki, Yasushi; Sato, Minoru; Sugiyama, Hitoshi; Okamoto, Kazunori; Maeshima, Yohei; Odawara, Masahiro; Sasaki, Junzo; Makino, Hirofumi.

In: Experimental Nephrology, Vol. 9, No. 5, 2001, p. 317-326.

Research output: Contribution to journalArticle

Maruyama, K, Kashihara, N, Yamasaki, Y, Sato, M, Sugiyama, H, Okamoto, K, Maeshima, Y, Odawara, M, Sasaki, J & Makino, H 2001, 'Methylprednisolone accelerates the resolution of glomerulonephritis by sensitizing mesangial cells to apoptosis', Experimental Nephrology, vol. 9, no. 5, pp. 317-326.
Maruyama, Keisuke ; Kashihara, Naoki ; Yamasaki, Yasushi ; Sato, Minoru ; Sugiyama, Hitoshi ; Okamoto, Kazunori ; Maeshima, Yohei ; Odawara, Masahiro ; Sasaki, Junzo ; Makino, Hirofumi. / Methylprednisolone accelerates the resolution of glomerulonephritis by sensitizing mesangial cells to apoptosis. In: Experimental Nephrology. 2001 ; Vol. 9, No. 5. pp. 317-326.
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AU - Sato, Minoru

AU - Sugiyama, Hitoshi

AU - Okamoto, Kazunori

AU - Maeshima, Yohei

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AU - Sasaki, Junzo

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AB - Glucocorticoid has long been used to treat patients with glomerulonephritis because it ameliorates mesangial cell proliferation and proteinuria, in part by suppressing nuclear factor-kappa B (NF-κB) activation, which regulates the transcription of various pro-inflammatory genes. Recent evidence shows that NF-κB activation increases the resistance to TNF-α-induced apoptosis in mesangial cells. We examined glomerular cell proliferation and apoptosis along with NF-κB activation in the Thy-1.1 nephritis model. We also evaluated TNF-α-induced apoptosis in cultured mesangial cells. Methylprednisolone treatment ameliorated mesangial hypercellularity in Thy-1.1 nephritis by decreasing proliferating cells and increasing apoptosis in the glomeruli. These effects were associated with suppressed NF-κB activation. This in vitro study revealed that treatment with methylprednisolone and TNF-α induced cultured mesangial cell apoptosis. These results suggest that methylprednisolone may accelerate the resolution phase of Thy-1.1 nephritis in part by sensitizing mesangial cells to apoptosis.

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