Methylobacterium oxalidis sp. nov., isolated from leaves of Oxalis corniculata

Akio Tani, Nurettin Sahin, Kazuhide Kimbara

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A pink-pigmented, facultatively methylotrophic bacterium, strain 35aT, was isolated from the leaves of Oxalis corniculata. Cells of strain 35aT were Gram-reaction-negative, motile, non-sporeforming rods. The highest 16S rRNA gene pairwise sequence similarities for strain 35aT were found with the strains of Methylobacterium iners 5317S-33T (96.7 %), 'Methylobacterium soli' YIM 48816 (96.6 %) and Methylobacterium jeotgali S2R03-9T (96.3 %). 16S rRNA gene sequence similarities with the type strains of all other recognized species of the genus Methylobacterium were below 96 %. Major cellular fatty acids were C18: 1ω7c, C18: 0 and C16: 0. The results of DNA-DNA hybridization experiments, analysis of cpn60 gene sequences, fatty acid profiles, whole-cell MALDI-TOF/MS spectral pattern analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 35aT from its nearest phylogenetic neighbours. Strain 35aT is therefore considered to represent a novel species within the genus Methylobacterium, for which the name Methylobacterium oxalidis sp. nov. is proposed. The type strain is 35aT (=DSM 24028T=NBRC 107715T).

Original languageEnglish
Pages (from-to)1647-1652
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume62
Issue number7
DOIs
Publication statusPublished - Jul 2012

Fingerprint

Oxalis corniculata
Methylobacterium
gene
new species
fatty acid
DNA
leaves
rRNA Genes
Fatty Acids
nucleotide sequences
phylogenetics
bacterium
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
ribosomal RNA
Names
Oxalidaceae
experiment
nucleic acid hybridization
Bacteria
analysis

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Methylobacterium oxalidis sp. nov., isolated from leaves of Oxalis corniculata. / Tani, Akio; Sahin, Nurettin; Kimbara, Kazuhide.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 62, No. 7, 07.2012, p. 1647-1652.

Research output: Contribution to journalArticle

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