Abstract
A pink-pigmented, facultatively methylotrophic bacterium, strain 23eT, was isolated from the leaves of Gnaphalium spicatum (cudweed). The cells of strain 23eT were Gram-reaction negative, motile and non-spore-forming rods. On the basis of 16S rRNA gene sequence similarities, strain 23eT was related to Methylobacterium organophilum ATCC 27886T (97.1 %) and Methylobacterium marchantiae JT1T (97 %), and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 97 %. Major cellular fatty acids were C18: 1ω7c, C16: 00 and C18: 0. The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrixassisted laser desorption/ionization time of flight/MS analysis, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 23eT from the phylogenetically closest relatives. We propose that strain 23eT represents a novel species within the genus Methylobacterium, for which the name Methylobacterium gnaphalii sp. nov. is proposed. The type strain is 23eT (=DSM 24027T=NBRC 107716T).
| Original language | English |
|---|---|
| Pages (from-to) | 2602-2607 |
| Number of pages | 6 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 62 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 1 2012 |
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ASJC Scopus subject areas
- Microbiology
- Ecology, Evolution, Behavior and Systematics
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Methylobacterium gnaphalii sp. nov., isolated from leaves of Gnaphalium spicatum. / Tani, Akio; Sahin, Nurettin; Kimbara, Kazuhide.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 62, No. 11, 01.11.2012, p. 2602-2607.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Methylobacterium gnaphalii sp. nov., isolated from leaves of Gnaphalium spicatum
AU - Tani, Akio
AU - Sahin, Nurettin
AU - Kimbara, Kazuhide
PY - 2012/11/1
Y1 - 2012/11/1
N2 - A pink-pigmented, facultatively methylotrophic bacterium, strain 23eT, was isolated from the leaves of Gnaphalium spicatum (cudweed). The cells of strain 23eT were Gram-reaction negative, motile and non-spore-forming rods. On the basis of 16S rRNA gene sequence similarities, strain 23eT was related to Methylobacterium organophilum ATCC 27886T (97.1 %) and Methylobacterium marchantiae JT1T (97 %), and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 97 %. Major cellular fatty acids were C18: 1ω7c, C16: 00 and C18: 0. The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrixassisted laser desorption/ionization time of flight/MS analysis, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 23eT from the phylogenetically closest relatives. We propose that strain 23eT represents a novel species within the genus Methylobacterium, for which the name Methylobacterium gnaphalii sp. nov. is proposed. The type strain is 23eT (=DSM 24027T=NBRC 107716T).
AB - A pink-pigmented, facultatively methylotrophic bacterium, strain 23eT, was isolated from the leaves of Gnaphalium spicatum (cudweed). The cells of strain 23eT were Gram-reaction negative, motile and non-spore-forming rods. On the basis of 16S rRNA gene sequence similarities, strain 23eT was related to Methylobacterium organophilum ATCC 27886T (97.1 %) and Methylobacterium marchantiae JT1T (97 %), and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 97 %. Major cellular fatty acids were C18: 1ω7c, C16: 00 and C18: 0. The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrixassisted laser desorption/ionization time of flight/MS analysis, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 23eT from the phylogenetically closest relatives. We propose that strain 23eT represents a novel species within the genus Methylobacterium, for which the name Methylobacterium gnaphalii sp. nov. is proposed. The type strain is 23eT (=DSM 24027T=NBRC 107716T).
UR - http://www.scopus.com/inward/record.url?scp=84868314689&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84868314689&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.037713-0
DO - 10.1099/ijs.0.037713-0
M3 - Article
C2 - 22199216
AN - SCOPUS:84868314689
VL - 62
SP - 2602
EP - 2607
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 11
ER -