Methylobacterium gnaphalii sp. nov., isolated from leaves of Gnaphalium spicatum

Akio Tani, Nurettin Sahin, Kazuhide Kimbara

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A pink-pigmented, facultatively methylotrophic bacterium, strain 23eT, was isolated from the leaves of Gnaphalium spicatum (cudweed). The cells of strain 23eT were Gram-reaction negative, motile and non-spore-forming rods. On the basis of 16S rRNA gene sequence similarities, strain 23eT was related to Methylobacterium organophilum ATCC 27886T (97.1 %) and Methylobacterium marchantiae JT1T (97 %), and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 97 %. Major cellular fatty acids were C18: 1ω7c, C16: 00 and C18: 0. The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrixassisted laser desorption/ionization time of flight/MS analysis, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 23eT from the phylogenetically closest relatives. We propose that strain 23eT represents a novel species within the genus Methylobacterium, for which the name Methylobacterium gnaphalii sp. nov. is proposed. The type strain is 23eT (=DSM 24027T=NBRC 107716T).

Original languageEnglish
Pages (from-to)2602-2607
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume62
Issue number11
DOIs
Publication statusPublished - Nov 1 2012

Fingerprint

Gnaphalium
Methylobacterium
fatty acid
phylogenetics
DNA
gene
new species
leaves
desorption
ionization
rRNA Genes
laser
Names
Fatty Acids
Methylobacterium organophilum
bacterium
Hepatophyta
ribosomal RNA
nucleotide sequences
phylogeny

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Methylobacterium gnaphalii sp. nov., isolated from leaves of Gnaphalium spicatum. / Tani, Akio; Sahin, Nurettin; Kimbara, Kazuhide.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 62, No. 11, 01.11.2012, p. 2602-2607.

Research output: Contribution to journalArticle

@article{024671f897aa45e29edddce4ec1742aa,
title = "Methylobacterium gnaphalii sp. nov., isolated from leaves of Gnaphalium spicatum",
abstract = "A pink-pigmented, facultatively methylotrophic bacterium, strain 23eT, was isolated from the leaves of Gnaphalium spicatum (cudweed). The cells of strain 23eT were Gram-reaction negative, motile and non-spore-forming rods. On the basis of 16S rRNA gene sequence similarities, strain 23eT was related to Methylobacterium organophilum ATCC 27886T (97.1 {\%}) and Methylobacterium marchantiae JT1T (97 {\%}), and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 97 {\%}. Major cellular fatty acids were C18: 1ω7c, C16: 00 and C18: 0. The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrixassisted laser desorption/ionization time of flight/MS analysis, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 23eT from the phylogenetically closest relatives. We propose that strain 23eT represents a novel species within the genus Methylobacterium, for which the name Methylobacterium gnaphalii sp. nov. is proposed. The type strain is 23eT (=DSM 24027T=NBRC 107716T).",
author = "Akio Tani and Nurettin Sahin and Kazuhide Kimbara",
year = "2012",
month = "11",
day = "1",
doi = "10.1099/ijs.0.037713-0",
language = "English",
volume = "62",
pages = "2602--2607",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "11",

}

TY - JOUR

T1 - Methylobacterium gnaphalii sp. nov., isolated from leaves of Gnaphalium spicatum

AU - Tani, Akio

AU - Sahin, Nurettin

AU - Kimbara, Kazuhide

PY - 2012/11/1

Y1 - 2012/11/1

N2 - A pink-pigmented, facultatively methylotrophic bacterium, strain 23eT, was isolated from the leaves of Gnaphalium spicatum (cudweed). The cells of strain 23eT were Gram-reaction negative, motile and non-spore-forming rods. On the basis of 16S rRNA gene sequence similarities, strain 23eT was related to Methylobacterium organophilum ATCC 27886T (97.1 %) and Methylobacterium marchantiae JT1T (97 %), and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 97 %. Major cellular fatty acids were C18: 1ω7c, C16: 00 and C18: 0. The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrixassisted laser desorption/ionization time of flight/MS analysis, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 23eT from the phylogenetically closest relatives. We propose that strain 23eT represents a novel species within the genus Methylobacterium, for which the name Methylobacterium gnaphalii sp. nov. is proposed. The type strain is 23eT (=DSM 24027T=NBRC 107716T).

AB - A pink-pigmented, facultatively methylotrophic bacterium, strain 23eT, was isolated from the leaves of Gnaphalium spicatum (cudweed). The cells of strain 23eT were Gram-reaction negative, motile and non-spore-forming rods. On the basis of 16S rRNA gene sequence similarities, strain 23eT was related to Methylobacterium organophilum ATCC 27886T (97.1 %) and Methylobacterium marchantiae JT1T (97 %), and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 97 %. Major cellular fatty acids were C18: 1ω7c, C16: 00 and C18: 0. The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrixassisted laser desorption/ionization time of flight/MS analysis, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 23eT from the phylogenetically closest relatives. We propose that strain 23eT represents a novel species within the genus Methylobacterium, for which the name Methylobacterium gnaphalii sp. nov. is proposed. The type strain is 23eT (=DSM 24027T=NBRC 107716T).

UR - http://www.scopus.com/inward/record.url?scp=84868314689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84868314689&partnerID=8YFLogxK

U2 - 10.1099/ijs.0.037713-0

DO - 10.1099/ijs.0.037713-0

M3 - Article

C2 - 22199216

AN - SCOPUS:84868314689

VL - 62

SP - 2602

EP - 2607

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 11

ER -