Methylobacterium gnaphalii sp. nov., isolated from leaves of Gnaphalium spicatum

Akio Tani, Nurettin Sahin, Kazuhide Kimbara

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A pink-pigmented, facultatively methylotrophic bacterium, strain 23eT, was isolated from the leaves of Gnaphalium spicatum (cudweed). The cells of strain 23eT were Gram-reaction negative, motile and non-spore-forming rods. On the basis of 16S rRNA gene sequence similarities, strain 23eT was related to Methylobacterium organophilum ATCC 27886T (97.1 %) and Methylobacterium marchantiae JT1T (97 %), and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 97 %. Major cellular fatty acids were C18: 1ω7c, C16: 00 and C18: 0. The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrixassisted laser desorption/ionization time of flight/MS analysis, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 23eT from the phylogenetically closest relatives. We propose that strain 23eT represents a novel species within the genus Methylobacterium, for which the name Methylobacterium gnaphalii sp. nov. is proposed. The type strain is 23eT (=DSM 24027T=NBRC 107716T).

Original languageEnglish
Pages (from-to)2602-2607
Number of pages6
JournalInternational journal of systematic and evolutionary microbiology
Volume62
Issue number11
DOIs
Publication statusPublished - Nov 1 2012

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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