Methylglyoxal-induced stomatal closure accompanied by peroxidase-mediated ROS production in Arabidopsis

Tahsina Sharmin Hoque, Misugi Uraji, Wenxiu Ye, Mohammad Anowar Hossain, Yoshimasa Nakamura, Yoshiyuki Murata

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Methylglyoxal (MG) is an oxygenated short aldehyde and a glycolytic intermediate that accumulates in plants under environmental stresses. Being a reactive α-oxoaldehyde, MG may act as a signaling molecule in plants during stresses. We investigated whether MG induces stomatal closure, reactive oxygen species (ROS) production, and cytosolic free calcium concentration ([Ca 2+] cyt) to clarify roles of MG in Arabidopsis guard cells. MG induced production of ROS and [Ca 2+] cyt oscillations, leading to stomatal closure. The MG-induced stomatal closure and ROS production were completely inhibited by a peroxidase inhibitor, salicylhydroxamic acid (SHAM), but were not affected by an NAD(P)H oxidase mutation, atrbohD atrbohF. Furthermore, the MG-elicited [Ca 2+] cyt oscillations were significantly suppressed by SHAM but not by the atrbohD atrbohF mutation. Neither endogenous abscisic acid nor endogenous methyl jasmonate was involved in MG-induced stomatal closure. These results suggest that intrinsic metabolite MG can induce stomatal closure in Arabidopsis accompanied by extracellular ROS production mediated by SHAM-sensitive peroxidases, intracellular ROS accumulation, and [Ca 2+] cyt oscillations.

Original languageEnglish
Pages (from-to)979-986
Number of pages8
JournalJournal of Plant Physiology
Volume169
Issue number10
DOIs
Publication statusPublished - Jul 1 2012

Keywords

  • Arabidopsis thaliana
  • ROS production
  • Stomatal closure
  • [Ca ] oscillation

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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