Methylation analysis of DNA mismatch repair genes using DNA derived from the peripheral blood of patients with endometrial cancer: Epimutation in endometrial carcinogenesis

Takashi Takeda, Kouji Banno, Megumi Yanokura, Masataka Adachi, Moito Iijima, Haruko Kunitomi, Kanako Nakamura, Miho Iida, Yuya Nogami, Kiyoko Umene, Kenta Masuda, Yusuke Kobayashi, Wataru Yamagami, Akira Hirasawa, Eiichiro Tominaga, Nobuyuki Susumu, Daisuke Aoki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Germline mutation of DNA mismatch repair (MMR) genes is a cause of Lynch syndrome. Methylation of MutL homolog 1 (MLH1) and MutS homolog 2 (MSH2) has been detected in peripheral blood cells of patients with colorectal cancer. This methylation is referred to as epimutation. Methylation of these genes has not been studied in an unselected series of endometrial cancer cases. Therefore, we examined methylation of MLH1, MSH2, and MSH6 promoter regions of peripheral blood cells in 206 patients with endometrial cancer using a methylation-specific polymerase chain reaction (MSP). Germline mutation of MMR genes, microsatellite instability (MSI), and immunohistochemistry (IHC) were also analyzed in each case with epimutation. MLH1 epimutation was detected in a single patient out of a total of 206 (0.49%) —1 out of 58 (1.72%) with an onset age of less than 50 years. The patient with MLH1 epimutation showed high level MSI (MSI-H), loss of MLH1 expression and had developed endometrial cancer at 46 years old, complicated with colorectal cancer. No case had epimutation of MSH2 or MSH6. The MLH1 epimutation detected in a patient with endometrial cancer may be a cause of endometrial carcinogenesis. This result indicates that it is important to check epimutation in patients with endometrial cancer without a germline mutation of MMR genes.

Original languageEnglish
Article number86
JournalGenes
Volume7
Issue number10
DOIs
Publication statusPublished - Oct 14 2016
Externally publishedYes

Keywords

  • Endometrial cancer
  • Epimutation
  • Lynch syndrome
  • Methylation
  • Mismatch repair genes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Takeda, T., Banno, K., Yanokura, M., Adachi, M., Iijima, M., Kunitomi, H., Nakamura, K., Iida, M., Nogami, Y., Umene, K., Masuda, K., Kobayashi, Y., Yamagami, W., Hirasawa, A., Tominaga, E., Susumu, N., & Aoki, D. (2016). Methylation analysis of DNA mismatch repair genes using DNA derived from the peripheral blood of patients with endometrial cancer: Epimutation in endometrial carcinogenesis. Genes, 7(10), [86]. https://doi.org/10.3390/genes7100086