Methionine-rich storage protein gene in the rice stem borer, Chilo suppressalis, is expressed during diapause in response to cold acclimation

S. Sonoda, K. Fukumoto, Y. Izumi, M. Ashfaq, H. Yoshida, H. Tsumuki

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    Gene expressions of acclimatized and non-acclimatized diapausing larvae were examined in Chilo suppressalis using a subtraction technique. A gene encoding a methionine-rich storage protein, CsSP1, was cloned and its complete cDNA sequence was determined. Potentially, CsSP1 encoded a 758-amino acid protein, with a calculated molecular weight of 88.8 kDa. The expression level of CsSP1 was higher in nondiapausing larvae than in diapausing ones. The CsSP1 expression was up-regulated in diapausing larvae when the temperature of cold acclimation was shifted to 5°C. The up-regulated level was maintained at 40 days after incubation at 5°C. In nondiapausing larvae, CsSP1 expression was down-regulated when the temperature was below developmental zero. Involvement of CsSP1 in diapause, cold tolerance acquisition and postdiapause development in C. suppressalis is discussed.

    Original languageEnglish
    Pages (from-to)853-859
    Number of pages7
    JournalInsect Molecular Biology
    Volume15
    Issue number6
    DOIs
    Publication statusPublished - Dec 1 2006

    Keywords

    • Chilo suppressalis
    • Cold acclimation
    • Diapause
    • Storage protein
    • Subtraction

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics
    • Insect Science

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