Abstract
Gene expressions of acclimatized and non-acclimatized diapausing larvae were examined in Chilo suppressalis using a subtraction technique. A gene encoding a methionine-rich storage protein, CsSP1, was cloned and its complete cDNA sequence was determined. Potentially, CsSP1 encoded a 758-amino acid protein, with a calculated molecular weight of 88.8 kDa. The expression level of CsSP1 was higher in nondiapausing larvae than in diapausing ones. The CsSP1 expression was up-regulated in diapausing larvae when the temperature of cold acclimation was shifted to 5°C. The up-regulated level was maintained at 40 days after incubation at 5°C. In nondiapausing larvae, CsSP1 expression was down-regulated when the temperature was below developmental zero. Involvement of CsSP1 in diapause, cold tolerance acquisition and postdiapause development in C. suppressalis is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 853-859 |
| Number of pages | 7 |
| Journal | Insect Molecular Biology |
| Volume | 15 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 2006 |
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Keywords
- Chilo suppressalis
- Cold acclimation
- Diapause
- Storage protein
- Subtraction
ASJC Scopus subject areas
- Insect Science
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
Cite this
Methionine-rich storage protein gene in the rice stem borer, Chilo suppressalis, is expressed during diapause in response to cold acclimation. / Sonoda, S.; Fukumoto, K.; Izumi, Y.; Ashfaq, M.; Yoshida, H.; Tsumuki, H.
In: Insect Molecular Biology, Vol. 15, No. 6, 12.2006, p. 853-859.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Methionine-rich storage protein gene in the rice stem borer, Chilo suppressalis, is expressed during diapause in response to cold acclimation
AU - Sonoda, S.
AU - Fukumoto, K.
AU - Izumi, Y.
AU - Ashfaq, M.
AU - Yoshida, H.
AU - Tsumuki, H.
PY - 2006/12
Y1 - 2006/12
N2 - Gene expressions of acclimatized and non-acclimatized diapausing larvae were examined in Chilo suppressalis using a subtraction technique. A gene encoding a methionine-rich storage protein, CsSP1, was cloned and its complete cDNA sequence was determined. Potentially, CsSP1 encoded a 758-amino acid protein, with a calculated molecular weight of 88.8 kDa. The expression level of CsSP1 was higher in nondiapausing larvae than in diapausing ones. The CsSP1 expression was up-regulated in diapausing larvae when the temperature of cold acclimation was shifted to 5°C. The up-regulated level was maintained at 40 days after incubation at 5°C. In nondiapausing larvae, CsSP1 expression was down-regulated when the temperature was below developmental zero. Involvement of CsSP1 in diapause, cold tolerance acquisition and postdiapause development in C. suppressalis is discussed.
AB - Gene expressions of acclimatized and non-acclimatized diapausing larvae were examined in Chilo suppressalis using a subtraction technique. A gene encoding a methionine-rich storage protein, CsSP1, was cloned and its complete cDNA sequence was determined. Potentially, CsSP1 encoded a 758-amino acid protein, with a calculated molecular weight of 88.8 kDa. The expression level of CsSP1 was higher in nondiapausing larvae than in diapausing ones. The CsSP1 expression was up-regulated in diapausing larvae when the temperature of cold acclimation was shifted to 5°C. The up-regulated level was maintained at 40 days after incubation at 5°C. In nondiapausing larvae, CsSP1 expression was down-regulated when the temperature was below developmental zero. Involvement of CsSP1 in diapause, cold tolerance acquisition and postdiapause development in C. suppressalis is discussed.
KW - Chilo suppressalis
KW - Cold acclimation
KW - Diapause
KW - Storage protein
KW - Subtraction
UR - http://www.scopus.com/inward/record.url?scp=33845636599&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845636599&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2583.2006.00670.x
DO - 10.1111/j.1365-2583.2006.00670.x
M3 - Article
C2 - 17201777
AN - SCOPUS:33845636599
VL - 15
SP - 853
EP - 859
JO - Insect Molecular Biology
JF - Insect Molecular Biology
SN - 0962-1075
IS - 6
ER -