Methionine-rich storage protein gene in the rice stem borer, Chilo suppressalis, is expressed during diapause in response to cold acclimation

S. Sonoda, K. Fukumoto, Y. Izumi, M. Ashfaq, H. Yoshida, H. Tsumuki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Gene expressions of acclimatized and non-acclimatized diapausing larvae were examined in Chilo suppressalis using a subtraction technique. A gene encoding a methionine-rich storage protein, CsSP1, was cloned and its complete cDNA sequence was determined. Potentially, CsSP1 encoded a 758-amino acid protein, with a calculated molecular weight of 88.8 kDa. The expression level of CsSP1 was higher in nondiapausing larvae than in diapausing ones. The CsSP1 expression was up-regulated in diapausing larvae when the temperature of cold acclimation was shifted to 5°C. The up-regulated level was maintained at 40 days after incubation at 5°C. In nondiapausing larvae, CsSP1 expression was down-regulated when the temperature was below developmental zero. Involvement of CsSP1 in diapause, cold tolerance acquisition and postdiapause development in C. suppressalis is discussed.

Original languageEnglish
Pages (from-to)853-859
Number of pages7
JournalInsect Molecular Biology
Volume15
Issue number6
DOIs
Publication statusPublished - Dec 1 2006

Keywords

  • Chilo suppressalis
  • Cold acclimation
  • Diapause
  • Storage protein
  • Subtraction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

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