Abstract
Gene expressions of acclimatized and non-acclimatized diapausing larvae were examined in Chilo suppressalis using a subtraction technique. A gene encoding a methionine-rich storage protein, CsSP1, was cloned and its complete cDNA sequence was determined. Potentially, CsSP1 encoded a 758-amino acid protein, with a calculated molecular weight of 88.8 kDa. The expression level of CsSP1 was higher in nondiapausing larvae than in diapausing ones. The CsSP1 expression was up-regulated in diapausing larvae when the temperature of cold acclimation was shifted to 5°C. The up-regulated level was maintained at 40 days after incubation at 5°C. In nondiapausing larvae, CsSP1 expression was down-regulated when the temperature was below developmental zero. Involvement of CsSP1 in diapause, cold tolerance acquisition and postdiapause development in C. suppressalis is discussed.
Original language | English |
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Pages (from-to) | 853-859 |
Number of pages | 7 |
Journal | Insect Molecular Biology |
Volume | 15 |
Issue number | 6 |
DOIs | |
Publication status | Published - Dec 1 2006 |
Keywords
- Chilo suppressalis
- Cold acclimation
- Diapause
- Storage protein
- Subtraction
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Insect Science