Methionine-rich storage protein gene in the rice stem borer, Chilo suppressalis, is expressed during diapause in response to cold acclimation

S. Sonoda, K. Fukumoto, Y. Izumi, M. Ashfaq, H. Yoshida, H. Tsumuki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Gene expressions of acclimatized and non-acclimatized diapausing larvae were examined in Chilo suppressalis using a subtraction technique. A gene encoding a methionine-rich storage protein, CsSP1, was cloned and its complete cDNA sequence was determined. Potentially, CsSP1 encoded a 758-amino acid protein, with a calculated molecular weight of 88.8 kDa. The expression level of CsSP1 was higher in nondiapausing larvae than in diapausing ones. The CsSP1 expression was up-regulated in diapausing larvae when the temperature of cold acclimation was shifted to 5°C. The up-regulated level was maintained at 40 days after incubation at 5°C. In nondiapausing larvae, CsSP1 expression was down-regulated when the temperature was below developmental zero. Involvement of CsSP1 in diapause, cold tolerance acquisition and postdiapause development in C. suppressalis is discussed.

Original languageEnglish
Pages (from-to)853-859
Number of pages7
JournalInsect Molecular Biology
Volume15
Issue number6
DOIs
Publication statusPublished - Dec 2006

Fingerprint

Chilo suppressalis
Acclimatization
storage proteins
diapause
Methionine
Larva
methionine
acclimation
Gene encoding
larvae
Gene expression
Proteins
genes
Complementary DNA
Molecular weight
Subtraction Technique
Amino Acids
Temperature
cold tolerance
temperature

Keywords

  • Chilo suppressalis
  • Cold acclimation
  • Diapause
  • Storage protein
  • Subtraction

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Methionine-rich storage protein gene in the rice stem borer, Chilo suppressalis, is expressed during diapause in response to cold acclimation. / Sonoda, S.; Fukumoto, K.; Izumi, Y.; Ashfaq, M.; Yoshida, H.; Tsumuki, H.

In: Insect Molecular Biology, Vol. 15, No. 6, 12.2006, p. 853-859.

Research output: Contribution to journalArticle

Sonoda, S. ; Fukumoto, K. ; Izumi, Y. ; Ashfaq, M. ; Yoshida, H. ; Tsumuki, H. / Methionine-rich storage protein gene in the rice stem borer, Chilo suppressalis, is expressed during diapause in response to cold acclimation. In: Insect Molecular Biology. 2006 ; Vol. 15, No. 6. pp. 853-859.
@article{78b5f70fac3c46459b0af6ed1225c838,
title = "Methionine-rich storage protein gene in the rice stem borer, Chilo suppressalis, is expressed during diapause in response to cold acclimation",
abstract = "Gene expressions of acclimatized and non-acclimatized diapausing larvae were examined in Chilo suppressalis using a subtraction technique. A gene encoding a methionine-rich storage protein, CsSP1, was cloned and its complete cDNA sequence was determined. Potentially, CsSP1 encoded a 758-amino acid protein, with a calculated molecular weight of 88.8 kDa. The expression level of CsSP1 was higher in nondiapausing larvae than in diapausing ones. The CsSP1 expression was up-regulated in diapausing larvae when the temperature of cold acclimation was shifted to 5°C. The up-regulated level was maintained at 40 days after incubation at 5°C. In nondiapausing larvae, CsSP1 expression was down-regulated when the temperature was below developmental zero. Involvement of CsSP1 in diapause, cold tolerance acquisition and postdiapause development in C. suppressalis is discussed.",
keywords = "Chilo suppressalis, Cold acclimation, Diapause, Storage protein, Subtraction",
author = "S. Sonoda and K. Fukumoto and Y. Izumi and M. Ashfaq and H. Yoshida and H. Tsumuki",
year = "2006",
month = "12",
doi = "10.1111/j.1365-2583.2006.00670.x",
language = "English",
volume = "15",
pages = "853--859",
journal = "Insect Molecular Biology",
issn = "0962-1075",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Methionine-rich storage protein gene in the rice stem borer, Chilo suppressalis, is expressed during diapause in response to cold acclimation

AU - Sonoda, S.

AU - Fukumoto, K.

AU - Izumi, Y.

AU - Ashfaq, M.

AU - Yoshida, H.

AU - Tsumuki, H.

PY - 2006/12

Y1 - 2006/12

N2 - Gene expressions of acclimatized and non-acclimatized diapausing larvae were examined in Chilo suppressalis using a subtraction technique. A gene encoding a methionine-rich storage protein, CsSP1, was cloned and its complete cDNA sequence was determined. Potentially, CsSP1 encoded a 758-amino acid protein, with a calculated molecular weight of 88.8 kDa. The expression level of CsSP1 was higher in nondiapausing larvae than in diapausing ones. The CsSP1 expression was up-regulated in diapausing larvae when the temperature of cold acclimation was shifted to 5°C. The up-regulated level was maintained at 40 days after incubation at 5°C. In nondiapausing larvae, CsSP1 expression was down-regulated when the temperature was below developmental zero. Involvement of CsSP1 in diapause, cold tolerance acquisition and postdiapause development in C. suppressalis is discussed.

AB - Gene expressions of acclimatized and non-acclimatized diapausing larvae were examined in Chilo suppressalis using a subtraction technique. A gene encoding a methionine-rich storage protein, CsSP1, was cloned and its complete cDNA sequence was determined. Potentially, CsSP1 encoded a 758-amino acid protein, with a calculated molecular weight of 88.8 kDa. The expression level of CsSP1 was higher in nondiapausing larvae than in diapausing ones. The CsSP1 expression was up-regulated in diapausing larvae when the temperature of cold acclimation was shifted to 5°C. The up-regulated level was maintained at 40 days after incubation at 5°C. In nondiapausing larvae, CsSP1 expression was down-regulated when the temperature was below developmental zero. Involvement of CsSP1 in diapause, cold tolerance acquisition and postdiapause development in C. suppressalis is discussed.

KW - Chilo suppressalis

KW - Cold acclimation

KW - Diapause

KW - Storage protein

KW - Subtraction

UR - http://www.scopus.com/inward/record.url?scp=33845636599&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845636599&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2583.2006.00670.x

DO - 10.1111/j.1365-2583.2006.00670.x

M3 - Article

C2 - 17201777

AN - SCOPUS:33845636599

VL - 15

SP - 853

EP - 859

JO - Insect Molecular Biology

JF - Insect Molecular Biology

SN - 0962-1075

IS - 6

ER -