Metal mesh vitrification (MMV) method for cryopreservation of porcine embryos

Y. Fujino, T. Kojima, Y. Nakamura, H. Kobayashi, K. Kikuchi, Hiroaki Funahashi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The objective was to develop a simpler, more reliable vitrification method for porcine embryos. Prepubertal donor gilts were induced to ovulate with eCG and hCG, and then inseminated artificially. Morulae and expanding blastocysts approximately 200 μm in diameter were collected 6 or 7 d after hCG treatment. Embryos collected from donor gilts were maintained, so as to be individually recognizable, and handled in batches of four or five. The embryos together with a minimum volume (

Original languageEnglish
Pages (from-to)809-817
Number of pages9
JournalTheriogenology
Volume70
Issue number5
DOIs
Publication statusPublished - Sep 15 2008

Fingerprint

Vitrification
Cryopreservation
vitrification
cryopreservation
embryo (animal)
Swine
Embryonic Structures
Metals
metals
gilts
swine
Morula
morula
Blastocyst
blastocyst
methodology
Therapeutics

Keywords

  • Cryopreservation
  • Metal mesh method
  • Porcine embryo
  • Vitrification

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Metal mesh vitrification (MMV) method for cryopreservation of porcine embryos. / Fujino, Y.; Kojima, T.; Nakamura, Y.; Kobayashi, H.; Kikuchi, K.; Funahashi, Hiroaki.

In: Theriogenology, Vol. 70, No. 5, 15.09.2008, p. 809-817.

Research output: Contribution to journalArticle

Fujino, Y. ; Kojima, T. ; Nakamura, Y. ; Kobayashi, H. ; Kikuchi, K. ; Funahashi, Hiroaki. / Metal mesh vitrification (MMV) method for cryopreservation of porcine embryos. In: Theriogenology. 2008 ; Vol. 70, No. 5. pp. 809-817.
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