Metagenomic Analysis Reveals Dynamic Changes of Whole Gut Microbiota in the Acute Phase of Intensive Care Unit Patients

Masahiro Ojima, Daisuke Motooka, Kentaro Shimizu, Kazuyoshi Goto, Ayumi Shintani, Kazuhisa Yoshiya, Shota Nakamura, Hiroshi Ogura, Tetsuya Iida, Takeshi Shimazu

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Background: Metagenomic analysis targeting the 16S rRNA gene has made it possible to characterize the vast array of microorganisms contained in the gut. Aim: The purpose of this study was to evaluate how gut microbiota change in intensive care unit (ICU) patients in the acute phase after admission. Methods: This prospective observational cohort study investigated 12 patients admitted to a single ICU of a large urban tertiary referral hospital. All patients were mechanically ventilated on admission. Fecal samples were collected from patients on days 1–2, 2–4, 5–8, and 7–10 after admission. DNA was extracted from fecal samples, and 16S rRNA deep sequencing was performed to monitor gut changes. Results: Bacteria belonging to the phyla Firmicutes or Bacteroidetes were predominant in each sample. We observed serial dynamic changes in the percentages of Bacteroidetes and Firmicutes that were significantly altered during study period (p <0.05). A ratio of Bacteroidetes to Firmicutes (B/F ratio) of >10 was seen in four of the six patients who died, whereas a B/F ratio of 10 or

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalDigestive Diseases and Sciences
DOIs
Publication statusAccepted/In press - Dec 29 2015
Externally publishedYes

Fingerprint

Metagenomics
Intensive Care Units
Bacteroidetes
High-Throughput Nucleotide Sequencing
Urban Hospitals
rRNA Genes
Tertiary Care Centers
Observational Studies
Cohort Studies
Gastrointestinal Microbiome
Bacteria
DNA

Keywords

  • Bacteroidetes
  • DNA sequencing
  • Intensive care
  • Metagenome
  • Metagenomics
  • Microbiota
  • RNA, Ribosomal, 16S
  • Sepsis

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Metagenomic Analysis Reveals Dynamic Changes of Whole Gut Microbiota in the Acute Phase of Intensive Care Unit Patients. / Ojima, Masahiro; Motooka, Daisuke; Shimizu, Kentaro; Goto, Kazuyoshi; Shintani, Ayumi; Yoshiya, Kazuhisa; Nakamura, Shota; Ogura, Hiroshi; Iida, Tetsuya; Shimazu, Takeshi.

In: Digestive Diseases and Sciences, 29.12.2015, p. 1-7.

Research output: Contribution to journalArticle

Ojima, Masahiro ; Motooka, Daisuke ; Shimizu, Kentaro ; Goto, Kazuyoshi ; Shintani, Ayumi ; Yoshiya, Kazuhisa ; Nakamura, Shota ; Ogura, Hiroshi ; Iida, Tetsuya ; Shimazu, Takeshi. / Metagenomic Analysis Reveals Dynamic Changes of Whole Gut Microbiota in the Acute Phase of Intensive Care Unit Patients. In: Digestive Diseases and Sciences. 2015 ; pp. 1-7.
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