Metabotropic glutamate receptors negatively regulate melatonin synthesis in rat pinealocytes

Hiroshi Yamada, Shouki Yatsushiro, Shougo Ishio, Mitsuko Hayashi, Tsuyoshi Nishi, Akitsugu Yamamoto, Masamitsu Futai, Akihito Yamaguchi, Yoshinori Moriyama

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Rat pinealocytes receive noradrenergic innervation that stimulates melatonin synthesis in a cAMP-mediated manner. In addition to melatonin, we showed previously that pinealocytes secrete L-glutamate through an exocytic mechanism. The released glutamate inhibits norepinephrine (NE)-dependent melatonin synthesis. Consistent with this observation, specific agonists of class II metabotropic glutamate receptors (mGluRs), including 1-(1S,3R)- aminocyclopentane-1,3-dicarboxylic acid (tACPD), inhibited NE-dependent melatonin synthesis, whereas agonists for other types of glutamate receptors did not. Furthermore, reverse transcription-PCR, Northern blotting, and immunohistochemistry analyses indicated expression of class II mGluR3 in pinealocytes. Inhibitory guanine nucleotide-binding protein (G(i)) was also detected in pinealocytes. L-Glutamate or agonists of class II receptors decreased NE- or forskolin-dependent increase of cAMP and serotonin-N- acetyltransferase activities to similar extents. These effects were blocked by pertussis toxin or dibutyryl cAMP. These results indicate that the inhibitory cAMP cascade is involved in the glutamate-evoked inhibition of melatonin synthesis. We propose that the glutaminergic system negatively regulates NE-dependent melatonin synthesis in rat pinealocytes.

Original languageEnglish
Pages (from-to)2056-2062
Number of pages7
JournalJournal of Neuroscience
Volume18
Issue number6
Publication statusPublished - Mar 15 1998
Externally publishedYes

Fingerprint

Metabotropic Glutamate Receptors
Melatonin
Glutamic Acid
Norepinephrine
Arylalkylamine N-Acetyltransferase
Excitatory Amino Acid Agonists
Dicarboxylic Acids
Guanine Nucleotides
Pertussis Toxin
Glutamate Receptors
Northern Blotting
Adrenergic Receptors
Reverse Transcription
Carrier Proteins
Immunohistochemistry
Polymerase Chain Reaction

Keywords

  • cAMP cascade
  • Endocrine cell
  • Melatonin
  • Metabotropic glutamate receptor
  • N- acetyltransferase
  • Pinealocyte

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Yamada, H., Yatsushiro, S., Ishio, S., Hayashi, M., Nishi, T., Yamamoto, A., ... Moriyama, Y. (1998). Metabotropic glutamate receptors negatively regulate melatonin synthesis in rat pinealocytes. Journal of Neuroscience, 18(6), 2056-2062.

Metabotropic glutamate receptors negatively regulate melatonin synthesis in rat pinealocytes. / Yamada, Hiroshi; Yatsushiro, Shouki; Ishio, Shougo; Hayashi, Mitsuko; Nishi, Tsuyoshi; Yamamoto, Akitsugu; Futai, Masamitsu; Yamaguchi, Akihito; Moriyama, Yoshinori.

In: Journal of Neuroscience, Vol. 18, No. 6, 15.03.1998, p. 2056-2062.

Research output: Contribution to journalArticle

Yamada, H, Yatsushiro, S, Ishio, S, Hayashi, M, Nishi, T, Yamamoto, A, Futai, M, Yamaguchi, A & Moriyama, Y 1998, 'Metabotropic glutamate receptors negatively regulate melatonin synthesis in rat pinealocytes', Journal of Neuroscience, vol. 18, no. 6, pp. 2056-2062.
Yamada H, Yatsushiro S, Ishio S, Hayashi M, Nishi T, Yamamoto A et al. Metabotropic glutamate receptors negatively regulate melatonin synthesis in rat pinealocytes. Journal of Neuroscience. 1998 Mar 15;18(6):2056-2062.
Yamada, Hiroshi ; Yatsushiro, Shouki ; Ishio, Shougo ; Hayashi, Mitsuko ; Nishi, Tsuyoshi ; Yamamoto, Akitsugu ; Futai, Masamitsu ; Yamaguchi, Akihito ; Moriyama, Yoshinori. / Metabotropic glutamate receptors negatively regulate melatonin synthesis in rat pinealocytes. In: Journal of Neuroscience. 1998 ; Vol. 18, No. 6. pp. 2056-2062.
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