Metabolism of Highly Persistent PCB Congener, 2,4,5,2′,4′,5′-Hexachlorobiphenyl, by Human CYP2B6

Noritaka Ariyoshi, K. Oguri, N. Koga, H. Yoshimura, Y. Funae

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Metabolism of 2,4,5,2′,4′,5′-hexachlorobiphenyl was studied with cDNA-expressed human P450 2B isoform, CYP2B6. 3-Hydroxy-2,4,5,2′,4′,5′-hexachlorobiphenyl was identified as a major metabolite, and the formation activity was compared with that of dog CYP2B11 and guinea pig P450GP-1. The activity of 3-hydroxylation was comparable with that of P450GP-1, but one-tenth of CYP2B11. These results indicate that P450 2B in humans as well as other animal species can metabolize 2,4,5,2′,4′,5′-hexachlorobiphenyl, and the reason why this PCB congener remained most abundantly in human bodies is discussed.

Original languageEnglish
Pages (from-to)455-460
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume212
Issue number2
DOIs
Publication statusPublished - Jul 17 1995
Externally publishedYes

Fingerprint

Polychlorinated Biphenyls
Metabolism
Hydroxylation
Metabolites
Human Body
Protein Isoforms
Guinea Pigs
Animals
Complementary DNA
Dogs
Cytochrome P-450 CYP2B6
cytochrome P-450 CYP2B11 (dog)

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Metabolism of Highly Persistent PCB Congener, 2,4,5,2′,4′,5′-Hexachlorobiphenyl, by Human CYP2B6. / Ariyoshi, Noritaka; Oguri, K.; Koga, N.; Yoshimura, H.; Funae, Y.

In: Biochemical and Biophysical Research Communications, Vol. 212, No. 2, 17.07.1995, p. 455-460.

Research output: Contribution to journalArticle

@article{14ac05a5d9da46a4a155d9d020881014,
title = "Metabolism of Highly Persistent PCB Congener, 2,4,5,2′,4′,5′-Hexachlorobiphenyl, by Human CYP2B6",
abstract = "Metabolism of 2,4,5,2′,4′,5′-hexachlorobiphenyl was studied with cDNA-expressed human P450 2B isoform, CYP2B6. 3-Hydroxy-2,4,5,2′,4′,5′-hexachlorobiphenyl was identified as a major metabolite, and the formation activity was compared with that of dog CYP2B11 and guinea pig P450GP-1. The activity of 3-hydroxylation was comparable with that of P450GP-1, but one-tenth of CYP2B11. These results indicate that P450 2B in humans as well as other animal species can metabolize 2,4,5,2′,4′,5′-hexachlorobiphenyl, and the reason why this PCB congener remained most abundantly in human bodies is discussed.",
author = "Noritaka Ariyoshi and K. Oguri and N. Koga and H. Yoshimura and Y. Funae",
year = "1995",
month = "7",
day = "17",
doi = "10.1006/bbrc.1995.1991",
language = "English",
volume = "212",
pages = "455--460",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Metabolism of Highly Persistent PCB Congener, 2,4,5,2′,4′,5′-Hexachlorobiphenyl, by Human CYP2B6

AU - Ariyoshi, Noritaka

AU - Oguri, K.

AU - Koga, N.

AU - Yoshimura, H.

AU - Funae, Y.

PY - 1995/7/17

Y1 - 1995/7/17

N2 - Metabolism of 2,4,5,2′,4′,5′-hexachlorobiphenyl was studied with cDNA-expressed human P450 2B isoform, CYP2B6. 3-Hydroxy-2,4,5,2′,4′,5′-hexachlorobiphenyl was identified as a major metabolite, and the formation activity was compared with that of dog CYP2B11 and guinea pig P450GP-1. The activity of 3-hydroxylation was comparable with that of P450GP-1, but one-tenth of CYP2B11. These results indicate that P450 2B in humans as well as other animal species can metabolize 2,4,5,2′,4′,5′-hexachlorobiphenyl, and the reason why this PCB congener remained most abundantly in human bodies is discussed.

AB - Metabolism of 2,4,5,2′,4′,5′-hexachlorobiphenyl was studied with cDNA-expressed human P450 2B isoform, CYP2B6. 3-Hydroxy-2,4,5,2′,4′,5′-hexachlorobiphenyl was identified as a major metabolite, and the formation activity was compared with that of dog CYP2B11 and guinea pig P450GP-1. The activity of 3-hydroxylation was comparable with that of P450GP-1, but one-tenth of CYP2B11. These results indicate that P450 2B in humans as well as other animal species can metabolize 2,4,5,2′,4′,5′-hexachlorobiphenyl, and the reason why this PCB congener remained most abundantly in human bodies is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0029123430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029123430&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1995.1991

DO - 10.1006/bbrc.1995.1991

M3 - Article

C2 - 7626059

AN - SCOPUS:0029123430

VL - 212

SP - 455

EP - 460

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -