Abstract
The metabolism of 2,3',4',5-tetrachlorobiphenyl (TCB) was compared using liver microsomes and six isoforms of cytochrome P450 purified from rats, guinea pigs and hamsters. In microsomal study, the following species differences were observed: 1) Untreated guinea pigs and hamsters but not rats can metabolize this TCB to 3-hydroxy- or 4-hydroxy-2,3',4',5-TCB, 2) Guinea pig microsomes showed only 3-hydroxylating activity, whereas hamster microsomes showed higher activity of 4-hydroxylation than that of 3- hydroxylation. In common with three species, the 3-hydroxylation was accelerated by phenobarbital. The 4-hydroxylation in rats and hamsters was increased by pretreatment with 3-methylcholanthrene and 3,3',4,4',5- pentachlorobiphenyl. The hydroxylation activities of liver microsomes from the three species could be explained by an involvement of different isoforms of cytochrome P450. In addition, it is apparent that hamster CYP1A2 as well as hamster CYP2A8 is involved in the 4-hydroxylation of 2,3',4',5-TCB although it has no activity for 2,2',5,5'-TCB.
| Original language | English |
|---|---|
| Pages (from-to) | 1895-1904 |
| Number of pages | 10 |
| Journal | Chemosphere |
| Volume | 37 |
| Issue number | 9-12 |
| DOIs | |
| Publication status | Published - Oct 1998 |
| Externally published | Yes |
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Keywords
- Cytochrome P450
- Guinea pig
- Hamster
- Metabolism
- Rat
- Tetrachlorobiphenyl
ASJC Scopus subject areas
- Environmental Chemistry
- Environmental Science(all)
Cite this
Metabolism of 2,3',4',5-tetrachlorobiphenyl by cytochrome P450 from rats, guinea pigs and hamsters. / Koga, Nobuyuki; Kikuichi, Naoko; Kanamaru, Tomoyo; Kuroki, Hiroaki; Matsusue, Kimihiko; Ishida, Chuzo; Ariyoshi, Noritaka; Oguri, Kazuta; Yoshimura, Hidetoshi.
In: Chemosphere, Vol. 37, No. 9-12, 10.1998, p. 1895-1904.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Metabolism of 2,3',4',5-tetrachlorobiphenyl by cytochrome P450 from rats, guinea pigs and hamsters
AU - Koga, Nobuyuki
AU - Kikuichi, Naoko
AU - Kanamaru, Tomoyo
AU - Kuroki, Hiroaki
AU - Matsusue, Kimihiko
AU - Ishida, Chuzo
AU - Ariyoshi, Noritaka
AU - Oguri, Kazuta
AU - Yoshimura, Hidetoshi
PY - 1998/10
Y1 - 1998/10
N2 - The metabolism of 2,3',4',5-tetrachlorobiphenyl (TCB) was compared using liver microsomes and six isoforms of cytochrome P450 purified from rats, guinea pigs and hamsters. In microsomal study, the following species differences were observed: 1) Untreated guinea pigs and hamsters but not rats can metabolize this TCB to 3-hydroxy- or 4-hydroxy-2,3',4',5-TCB, 2) Guinea pig microsomes showed only 3-hydroxylating activity, whereas hamster microsomes showed higher activity of 4-hydroxylation than that of 3- hydroxylation. In common with three species, the 3-hydroxylation was accelerated by phenobarbital. The 4-hydroxylation in rats and hamsters was increased by pretreatment with 3-methylcholanthrene and 3,3',4,4',5- pentachlorobiphenyl. The hydroxylation activities of liver microsomes from the three species could be explained by an involvement of different isoforms of cytochrome P450. In addition, it is apparent that hamster CYP1A2 as well as hamster CYP2A8 is involved in the 4-hydroxylation of 2,3',4',5-TCB although it has no activity for 2,2',5,5'-TCB.
AB - The metabolism of 2,3',4',5-tetrachlorobiphenyl (TCB) was compared using liver microsomes and six isoforms of cytochrome P450 purified from rats, guinea pigs and hamsters. In microsomal study, the following species differences were observed: 1) Untreated guinea pigs and hamsters but not rats can metabolize this TCB to 3-hydroxy- or 4-hydroxy-2,3',4',5-TCB, 2) Guinea pig microsomes showed only 3-hydroxylating activity, whereas hamster microsomes showed higher activity of 4-hydroxylation than that of 3- hydroxylation. In common with three species, the 3-hydroxylation was accelerated by phenobarbital. The 4-hydroxylation in rats and hamsters was increased by pretreatment with 3-methylcholanthrene and 3,3',4,4',5- pentachlorobiphenyl. The hydroxylation activities of liver microsomes from the three species could be explained by an involvement of different isoforms of cytochrome P450. In addition, it is apparent that hamster CYP1A2 as well as hamster CYP2A8 is involved in the 4-hydroxylation of 2,3',4',5-TCB although it has no activity for 2,2',5,5'-TCB.
KW - Cytochrome P450
KW - Guinea pig
KW - Hamster
KW - Metabolism
KW - Rat
KW - Tetrachlorobiphenyl
UR - http://www.scopus.com/inward/record.url?scp=0032190760&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032190760&partnerID=8YFLogxK
U2 - 10.1016/S0045-6535(98)00256-2
DO - 10.1016/S0045-6535(98)00256-2
M3 - Article
C2 - 9828318
AN - SCOPUS:0032190760
VL - 37
SP - 1895
EP - 1904
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
IS - 9-12
ER -