Metabolism of 2,3',4',5-tetrachlorobiphenyl by cytochrome P450 from rats, guinea pigs and hamsters

Nobuyuki Koga, Naoko Kikuichi, Tomoyo Kanamaru, Hiroaki Kuroki, Kimihiko Matsusue, Chuzo Ishida, Noritaka Ariyoshi, Kazuta Oguri, Hidetoshi Yoshimura

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The metabolism of 2,3',4',5-tetrachlorobiphenyl (TCB) was compared using liver microsomes and six isoforms of cytochrome P450 purified from rats, guinea pigs and hamsters. In microsomal study, the following species differences were observed: 1) Untreated guinea pigs and hamsters but not rats can metabolize this TCB to 3-hydroxy- or 4-hydroxy-2,3',4',5-TCB, 2) Guinea pig microsomes showed only 3-hydroxylating activity, whereas hamster microsomes showed higher activity of 4-hydroxylation than that of 3- hydroxylation. In common with three species, the 3-hydroxylation was accelerated by phenobarbital. The 4-hydroxylation in rats and hamsters was increased by pretreatment with 3-methylcholanthrene and 3,3',4,4',5- pentachlorobiphenyl. The hydroxylation activities of liver microsomes from the three species could be explained by an involvement of different isoforms of cytochrome P450. In addition, it is apparent that hamster CYP1A2 as well as hamster CYP2A8 is involved in the 4-hydroxylation of 2,3',4',5-TCB although it has no activity for 2,2',5,5'-TCB.

Original languageEnglish
Pages (from-to)1895-1904
Number of pages10
JournalChemosphere
Volume37
Issue number9-12
DOIs
Publication statusPublished - Oct 1 1998
Externally publishedYes

Keywords

  • Cytochrome P450
  • Guinea pig
  • Hamster
  • Metabolism
  • Rat
  • Tetrachlorobiphenyl

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

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