Metabolic movement upon Abscisic acid and Salicylic acid combined treatments

Mami Okamoto, Yuuri Tsuboi, Eisuke Chikayama, Jun Kikuchi, Takashi Hirayama

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Plant hormones are known to play important roles for maintenance of internal conditions under various environmental stresses. Recent studies revealed that there is a significant cross-talk between abiotic and biotic stress responses. To understand such complex mechanisms, comprehensive analyses at multiple levels are required. In this study, to examine the dynamic interactions between plant hormone responses, we analyzed the metabolic movements of Arabidopsis thaliana cultured cells during hormone treatments by NMR metabolic profiling. First, we verified the effect of plant hormone treatments on intracellular metabolites, and detected that the abscisic acid (ABA), salicylic acid (SA), auxin, and brassinosteroid treatment caused metabolic changes. Secondly, since SA and ABA act antagonistically against each other, we monitored dynamic metabolic movements during ABA and SA combined treatments. The response to ABA-only treatment suggested that sugars and amino acids significantly increased. Although SA alone caused fewer metabolic changes, SA caused remarkable metabolic changes when applied in combination with ABA. In addition, our NMR data implied that salicylate glucoside (SAG), which is major metabolite converted from SA, significantly increased in the SA-only treatment but decreased with ABA in a dose dependent manner. These results suggest that ABA and SA cross-talk at the metabolite level in a complicate manner and that the combination of various conditions will provide us with a holistic view of plant stress response mechanisms.

Original languageEnglish
Pages (from-to)551-560
Number of pages10
JournalPlant Biotechnology
Volume26
Issue number5
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Abscisic Acid
Salicylic Acid
salicylic acid
abscisic acid
Plant Growth Regulators
plant hormones
metabolites
stress response
Brassinosteroids
Sugar Acids
salicylates
Indoleacetic Acids
brassinosteroids
Salicylates
sugar acids
plant stress
Glucosides
metabolomics
biotic stress
acid treatment

Keywords

  • Cultured cell
  • Metabolome
  • NMR
  • Plant hormone

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology
  • Agronomy and Crop Science

Cite this

Okamoto, M., Tsuboi, Y., Chikayama, E., Kikuchi, J., & Hirayama, T. (2009). Metabolic movement upon Abscisic acid and Salicylic acid combined treatments. Plant Biotechnology, 26(5), 551-560.

Metabolic movement upon Abscisic acid and Salicylic acid combined treatments. / Okamoto, Mami; Tsuboi, Yuuri; Chikayama, Eisuke; Kikuchi, Jun; Hirayama, Takashi.

In: Plant Biotechnology, Vol. 26, No. 5, 2009, p. 551-560.

Research output: Contribution to journalArticle

Okamoto, M, Tsuboi, Y, Chikayama, E, Kikuchi, J & Hirayama, T 2009, 'Metabolic movement upon Abscisic acid and Salicylic acid combined treatments', Plant Biotechnology, vol. 26, no. 5, pp. 551-560.
Okamoto, Mami ; Tsuboi, Yuuri ; Chikayama, Eisuke ; Kikuchi, Jun ; Hirayama, Takashi. / Metabolic movement upon Abscisic acid and Salicylic acid combined treatments. In: Plant Biotechnology. 2009 ; Vol. 26, No. 5. pp. 551-560.
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