Mesenchymal stem/progenitor cell isolation from tooth extraction sockets

R. Nakajima, Mitsuaki Ono, Emilio satoshi Hara, Y. Oida, S. Shinkawa, H. T. Pham, Kentaro Akiyama, W. Sonoyama, Kenji Maekawa, Takuo Kuboki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Bone marrow-derived mesenchymal stem/progenitor cells (BMSCs) are commonly used in regeneration therapy. The current primary source of BMSCs is the iliac crest; however, the procedure is associated with various burdens on the patient, including the risk of pain and infection. Hence, the possibility to collect BMSCs from other, more accessible, sources would be an attractive approach. It is well known that stem cells migrate from surrounding tissues and play important roles in wound healing. We thus hypothesized that stem/progenitor cells could be isolated from granulation tissue in the dental socket, and we subsequently collected granulation tissue from dog dental socket 3 d after tooth extraction. After enzyme digestion of the collected tissue, the cells forming colonies constituted the dental socket-derived stem/progenitor cells (dDSCs). Next, dDSCs were compared with dog BMSCs (dBMSCs) for phenotype characterization. A flow cytometric analysis showed that dDSCs were positive for CD44, CD90, and CD271 but negative for CD34 and CD45, similar to dBMSCs. dDSCs also exhibited osteogenic, adipogenic, and chondrogenic differentiation ability, similar to dBMSCs, with a higher capacity for colony formation, proliferation, and motility than dBMSCs. In addition, an in vivo ectopic bone formation assay showed that dDSCs and dBMSCs both induced hard tissue formation, although only dDSCs formed a fibrous tissue-like structure connected to the newly formed bone. Finally, we tested the ability of dDSCs to regenerate periodontal tissue in a one-wall defect model. The defects in the dDSC-transplanted group (β-TCP/PGA/dDSCs) were regenerated with cementum-like and periodontal ligament-like tissues and alveolar bone, whereas only bony tissue was observed in the control group (β-TCP/PGA). In conclusion, we identified and characterized a population of stem/progenitor cells in granulation tissue obtained from the dental socket that exhibited several characteristics similar to those of BMSCs. Dental sockets could therefore be a novel source for isolating stem/progenitor cells from bone.

Original languageEnglish
Pages (from-to)1133-1140
Number of pages8
JournalJournal of Dental Research
Volume93
Issue number11
DOIs
Publication statusPublished - Nov 11 2014

Fingerprint

Tooth Socket
Tooth Extraction
Cell Separation
Mesenchymal Stromal Cells
Stem Cells
Dogs
Bone Marrow
Granulation Tissue
Prostaglandins A
Aptitude

Keywords

  • bone marrow-derived stem cells
  • dental socket
  • dental tissue-derived mesenchymal stem cells
  • granulation tissue
  • stem cells
  • wound healing

ASJC Scopus subject areas

  • Dentistry(all)
  • Medicine(all)

Cite this

Mesenchymal stem/progenitor cell isolation from tooth extraction sockets. / Nakajima, R.; Ono, Mitsuaki; Hara, Emilio satoshi; Oida, Y.; Shinkawa, S.; Pham, H. T.; Akiyama, Kentaro; Sonoyama, W.; Maekawa, Kenji; Kuboki, Takuo.

In: Journal of Dental Research, Vol. 93, No. 11, 11.11.2014, p. 1133-1140.

Research output: Contribution to journalArticle

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AU - Shinkawa, S.

AU - Pham, H. T.

AU - Akiyama, Kentaro

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AU - Maekawa, Kenji

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