Abstract
Summary Langerhans cell histiocytosis (LCH) is a group of granulomatous disorders in which abnormal Langerhans cells proliferate as either a localized lesion in a single bone or disseminated disease involving two or more organs or systems. Because the different LCH forms exhibit significantly elevated levels of inflammatory molecules, including pro-inflammatory cytokines and tissue-degrading enzymes, we investigated for a possible viral trigger in LCH pathogenesis. We looked for Merkel cell polyomavirus (MCPyV) in peripheral blood cells and tissues using quantitative real-time PCR and immunohistochemistry staining with anti-MCPyV large T-antigen antibody. Our findings revealed elevated amounts of MCPyV DNA in the peripheral blood cells of 2 of 3 patients affected by LCH with high-risk organ involvement (RO+) and absence of MCPyV DNA in the blood cells in all 12 LCH-RO- patients (P =.029). With lower viral loads (0.002-0.033 copies/cell), an elevated number of MCPyV DNA sequences was detected in 12 LCH tissues in comparison with control tissues obtained from patients with reactive lymphoid hyperplasia (0/5; P =.0007), skin diseases not related to LCH in children younger than 2 years (0/11; P =.0007), or dermatopathic lymphadenopathy (5/20; P =.0002). The data, including frequent but lower viral loads and low large-T antigen expression rate (2/13 LCH tissues), suggest that development of LCH as a reactive rather than a neoplastic process may be related to MCPyV infection.
| Original language | English |
|---|---|
| Pages (from-to) | 119-126 |
| Number of pages | 8 |
| Journal | Human Pathology |
| Volume | 45 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2014 |
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Keywords
- Dermatopathic lymphadenopathy
- Langerhans cell histiocytosis
- Langerhans cells
- Merkel cell polyomavirus
- Multiplex quantitative real-time PCR
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Merkel cell polyomavirus DNA sequences in peripheral blood and tissues from patients with Langerhans cell histiocytosis. / Murakami, Ichiro; Matsushita, Michiko; Iwasaki, Takeshi; Kuwamoto, Satoshi; Kato, Masako; Horie, Yasushi; Hayashi, Kazuhiko; Imamura, Toshihiko; Morimoto, Akira; Imashuku, Shinsaku; Gogusev, Jean; Jaubert, Francis; Takata, Katsuyoshi; Oka, Takashi; Yoshino, Tadashi.
In: Human Pathology, Vol. 45, No. 1, 01.2014, p. 119-126.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Merkel cell polyomavirus DNA sequences in peripheral blood and tissues from patients with Langerhans cell histiocytosis
AU - Murakami, Ichiro
AU - Matsushita, Michiko
AU - Iwasaki, Takeshi
AU - Kuwamoto, Satoshi
AU - Kato, Masako
AU - Horie, Yasushi
AU - Hayashi, Kazuhiko
AU - Imamura, Toshihiko
AU - Morimoto, Akira
AU - Imashuku, Shinsaku
AU - Gogusev, Jean
AU - Jaubert, Francis
AU - Takata, Katsuyoshi
AU - Oka, Takashi
AU - Yoshino, Tadashi
PY - 2014/1
Y1 - 2014/1
N2 - Summary Langerhans cell histiocytosis (LCH) is a group of granulomatous disorders in which abnormal Langerhans cells proliferate as either a localized lesion in a single bone or disseminated disease involving two or more organs or systems. Because the different LCH forms exhibit significantly elevated levels of inflammatory molecules, including pro-inflammatory cytokines and tissue-degrading enzymes, we investigated for a possible viral trigger in LCH pathogenesis. We looked for Merkel cell polyomavirus (MCPyV) in peripheral blood cells and tissues using quantitative real-time PCR and immunohistochemistry staining with anti-MCPyV large T-antigen antibody. Our findings revealed elevated amounts of MCPyV DNA in the peripheral blood cells of 2 of 3 patients affected by LCH with high-risk organ involvement (RO+) and absence of MCPyV DNA in the blood cells in all 12 LCH-RO- patients (P =.029). With lower viral loads (0.002-0.033 copies/cell), an elevated number of MCPyV DNA sequences was detected in 12 LCH tissues in comparison with control tissues obtained from patients with reactive lymphoid hyperplasia (0/5; P =.0007), skin diseases not related to LCH in children younger than 2 years (0/11; P =.0007), or dermatopathic lymphadenopathy (5/20; P =.0002). The data, including frequent but lower viral loads and low large-T antigen expression rate (2/13 LCH tissues), suggest that development of LCH as a reactive rather than a neoplastic process may be related to MCPyV infection.
AB - Summary Langerhans cell histiocytosis (LCH) is a group of granulomatous disorders in which abnormal Langerhans cells proliferate as either a localized lesion in a single bone or disseminated disease involving two or more organs or systems. Because the different LCH forms exhibit significantly elevated levels of inflammatory molecules, including pro-inflammatory cytokines and tissue-degrading enzymes, we investigated for a possible viral trigger in LCH pathogenesis. We looked for Merkel cell polyomavirus (MCPyV) in peripheral blood cells and tissues using quantitative real-time PCR and immunohistochemistry staining with anti-MCPyV large T-antigen antibody. Our findings revealed elevated amounts of MCPyV DNA in the peripheral blood cells of 2 of 3 patients affected by LCH with high-risk organ involvement (RO+) and absence of MCPyV DNA in the blood cells in all 12 LCH-RO- patients (P =.029). With lower viral loads (0.002-0.033 copies/cell), an elevated number of MCPyV DNA sequences was detected in 12 LCH tissues in comparison with control tissues obtained from patients with reactive lymphoid hyperplasia (0/5; P =.0007), skin diseases not related to LCH in children younger than 2 years (0/11; P =.0007), or dermatopathic lymphadenopathy (5/20; P =.0002). The data, including frequent but lower viral loads and low large-T antigen expression rate (2/13 LCH tissues), suggest that development of LCH as a reactive rather than a neoplastic process may be related to MCPyV infection.
KW - Dermatopathic lymphadenopathy
KW - Langerhans cell histiocytosis
KW - Langerhans cells
KW - Merkel cell polyomavirus
KW - Multiplex quantitative real-time PCR
UR - http://www.scopus.com/inward/record.url?scp=84890071984&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84890071984&partnerID=8YFLogxK
U2 - 10.1016/j.humpath.2013.05.028
DO - 10.1016/j.humpath.2013.05.028
M3 - Article
C2 - 24321520
AN - SCOPUS:84890071984
VL - 45
SP - 119
EP - 126
JO - Human Pathology
JF - Human Pathology
SN - 0046-8177
IS - 1
ER -