Memory-related gene expression profile of the male rat hippocampus induced by teeth extraction and occlusal support recovery

Sachiyo Iida, Tetsuya Hara, Daisuke Araki, Chisa Ishimine-Kuroda, Akimasa Kurozumi, Shunichi Sakamoto, Takako Miyazaki, Shougo Minagi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives The present study aimed to identify the effect of memory-related genes on male rats tested for spatial memory with either molar teeth extraction or its restoration by occlusal support using experimental dentures. Design Memory-related genes were detected from hippocampi of male Wistar rats (exposed to teeth extraction with or without dentures, or no extraction (control)) (7-week old) after behavioural testing (via the radial maze task) using a DNA microarray. The time course of the expression of these genes was evaluated by quantitative real-time polymerase chain reaction (PCR) (on 49-week-old rats). Results In preliminary experiments, to determine which memory genes are affected by spatial memory training, DNA microarray analysis revealed that thyrotropin-releasing hormone (Trh) and tenascin XA (Tnxa) were up-regulated and neuronatin (Nnat) and S100a9 were down-regulated after the maze training. The expression of Tnxa, Nnat and S100a9 of 49-week-old rats (during the time course) via quantitative real-time PCR was consistent with the results of microarrays of the preliminary experiment. Expression of Trh that was evaluated by quantitative real-time PCR did not agree with the results for this gene from the microarray for all groups. Therefore, expression of Trh may have increased in only young, trained rats. The expression of S100a9 prior to the maze task was down-regulated in only the extraction group. Conclusion These results demonstrated that Trh, Tnxa and Nnat genes were affected according to the degree of memory in male rats. This study also indicated that S100a9 is a memory-related gene, which is affected by the presence of occlusal support.

Original languageEnglish
Pages (from-to)133-141
Number of pages9
JournalArchives of Oral Biology
Volume59
Issue number2
DOIs
Publication statusPublished - Feb 2014

Fingerprint

Tooth Extraction
Transcriptome
Hippocampus
Thyrotropin-Releasing Hormone
Tenascin
Genes
Real-Time Polymerase Chain Reaction
Dentures
Oligonucleotide Array Sequence Analysis
Microarray Analysis
Wistar Rats
Learning
Gene Expression

Keywords

  • Gene expression
  • Hippocampus
  • Memory and learning
  • Nnat
  • S100a9
  • Tnxa
  • Trh

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Cell Biology
  • Dentistry(all)

Cite this

Memory-related gene expression profile of the male rat hippocampus induced by teeth extraction and occlusal support recovery. / Iida, Sachiyo; Hara, Tetsuya; Araki, Daisuke; Ishimine-Kuroda, Chisa; Kurozumi, Akimasa; Sakamoto, Shunichi; Miyazaki, Takako; Minagi, Shougo.

In: Archives of Oral Biology, Vol. 59, No. 2, 02.2014, p. 133-141.

Research output: Contribution to journalArticle

Iida, Sachiyo ; Hara, Tetsuya ; Araki, Daisuke ; Ishimine-Kuroda, Chisa ; Kurozumi, Akimasa ; Sakamoto, Shunichi ; Miyazaki, Takako ; Minagi, Shougo. / Memory-related gene expression profile of the male rat hippocampus induced by teeth extraction and occlusal support recovery. In: Archives of Oral Biology. 2014 ; Vol. 59, No. 2. pp. 133-141.
@article{a6aed9038fe24fa1a7ba075bf1dcf097,
title = "Memory-related gene expression profile of the male rat hippocampus induced by teeth extraction and occlusal support recovery",
abstract = "Objectives The present study aimed to identify the effect of memory-related genes on male rats tested for spatial memory with either molar teeth extraction or its restoration by occlusal support using experimental dentures. Design Memory-related genes were detected from hippocampi of male Wistar rats (exposed to teeth extraction with or without dentures, or no extraction (control)) (7-week old) after behavioural testing (via the radial maze task) using a DNA microarray. The time course of the expression of these genes was evaluated by quantitative real-time polymerase chain reaction (PCR) (on 49-week-old rats). Results In preliminary experiments, to determine which memory genes are affected by spatial memory training, DNA microarray analysis revealed that thyrotropin-releasing hormone (Trh) and tenascin XA (Tnxa) were up-regulated and neuronatin (Nnat) and S100a9 were down-regulated after the maze training. The expression of Tnxa, Nnat and S100a9 of 49-week-old rats (during the time course) via quantitative real-time PCR was consistent with the results of microarrays of the preliminary experiment. Expression of Trh that was evaluated by quantitative real-time PCR did not agree with the results for this gene from the microarray for all groups. Therefore, expression of Trh may have increased in only young, trained rats. The expression of S100a9 prior to the maze task was down-regulated in only the extraction group. Conclusion These results demonstrated that Trh, Tnxa and Nnat genes were affected according to the degree of memory in male rats. This study also indicated that S100a9 is a memory-related gene, which is affected by the presence of occlusal support.",
keywords = "Gene expression, Hippocampus, Memory and learning, Nnat, S100a9, Tnxa, Trh",
author = "Sachiyo Iida and Tetsuya Hara and Daisuke Araki and Chisa Ishimine-Kuroda and Akimasa Kurozumi and Shunichi Sakamoto and Takako Miyazaki and Shougo Minagi",
year = "2014",
month = "2",
doi = "10.1016/j.archoralbio.2013.10.003",
language = "English",
volume = "59",
pages = "133--141",
journal = "Archives of Oral Biology",
issn = "0003-9969",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Memory-related gene expression profile of the male rat hippocampus induced by teeth extraction and occlusal support recovery

AU - Iida, Sachiyo

AU - Hara, Tetsuya

AU - Araki, Daisuke

AU - Ishimine-Kuroda, Chisa

AU - Kurozumi, Akimasa

AU - Sakamoto, Shunichi

AU - Miyazaki, Takako

AU - Minagi, Shougo

PY - 2014/2

Y1 - 2014/2

N2 - Objectives The present study aimed to identify the effect of memory-related genes on male rats tested for spatial memory with either molar teeth extraction or its restoration by occlusal support using experimental dentures. Design Memory-related genes were detected from hippocampi of male Wistar rats (exposed to teeth extraction with or without dentures, or no extraction (control)) (7-week old) after behavioural testing (via the radial maze task) using a DNA microarray. The time course of the expression of these genes was evaluated by quantitative real-time polymerase chain reaction (PCR) (on 49-week-old rats). Results In preliminary experiments, to determine which memory genes are affected by spatial memory training, DNA microarray analysis revealed that thyrotropin-releasing hormone (Trh) and tenascin XA (Tnxa) were up-regulated and neuronatin (Nnat) and S100a9 were down-regulated after the maze training. The expression of Tnxa, Nnat and S100a9 of 49-week-old rats (during the time course) via quantitative real-time PCR was consistent with the results of microarrays of the preliminary experiment. Expression of Trh that was evaluated by quantitative real-time PCR did not agree with the results for this gene from the microarray for all groups. Therefore, expression of Trh may have increased in only young, trained rats. The expression of S100a9 prior to the maze task was down-regulated in only the extraction group. Conclusion These results demonstrated that Trh, Tnxa and Nnat genes were affected according to the degree of memory in male rats. This study also indicated that S100a9 is a memory-related gene, which is affected by the presence of occlusal support.

AB - Objectives The present study aimed to identify the effect of memory-related genes on male rats tested for spatial memory with either molar teeth extraction or its restoration by occlusal support using experimental dentures. Design Memory-related genes were detected from hippocampi of male Wistar rats (exposed to teeth extraction with or without dentures, or no extraction (control)) (7-week old) after behavioural testing (via the radial maze task) using a DNA microarray. The time course of the expression of these genes was evaluated by quantitative real-time polymerase chain reaction (PCR) (on 49-week-old rats). Results In preliminary experiments, to determine which memory genes are affected by spatial memory training, DNA microarray analysis revealed that thyrotropin-releasing hormone (Trh) and tenascin XA (Tnxa) were up-regulated and neuronatin (Nnat) and S100a9 were down-regulated after the maze training. The expression of Tnxa, Nnat and S100a9 of 49-week-old rats (during the time course) via quantitative real-time PCR was consistent with the results of microarrays of the preliminary experiment. Expression of Trh that was evaluated by quantitative real-time PCR did not agree with the results for this gene from the microarray for all groups. Therefore, expression of Trh may have increased in only young, trained rats. The expression of S100a9 prior to the maze task was down-regulated in only the extraction group. Conclusion These results demonstrated that Trh, Tnxa and Nnat genes were affected according to the degree of memory in male rats. This study also indicated that S100a9 is a memory-related gene, which is affected by the presence of occlusal support.

KW - Gene expression

KW - Hippocampus

KW - Memory and learning

KW - Nnat

KW - S100a9

KW - Tnxa

KW - Trh

UR - http://www.scopus.com/inward/record.url?scp=84889238192&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84889238192&partnerID=8YFLogxK

U2 - 10.1016/j.archoralbio.2013.10.003

DO - 10.1016/j.archoralbio.2013.10.003

M3 - Article

VL - 59

SP - 133

EP - 141

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

IS - 2

ER -