Abstract
Objectives The present study aimed to identify the effect of memory-related genes on male rats tested for spatial memory with either molar teeth extraction or its restoration by occlusal support using experimental dentures. Design Memory-related genes were detected from hippocampi of male Wistar rats (exposed to teeth extraction with or without dentures, or no extraction (control)) (7-week old) after behavioural testing (via the radial maze task) using a DNA microarray. The time course of the expression of these genes was evaluated by quantitative real-time polymerase chain reaction (PCR) (on 49-week-old rats). Results In preliminary experiments, to determine which memory genes are affected by spatial memory training, DNA microarray analysis revealed that thyrotropin-releasing hormone (Trh) and tenascin XA (Tnxa) were up-regulated and neuronatin (Nnat) and S100a9 were down-regulated after the maze training. The expression of Tnxa, Nnat and S100a9 of 49-week-old rats (during the time course) via quantitative real-time PCR was consistent with the results of microarrays of the preliminary experiment. Expression of Trh that was evaluated by quantitative real-time PCR did not agree with the results for this gene from the microarray for all groups. Therefore, expression of Trh may have increased in only young, trained rats. The expression of S100a9 prior to the maze task was down-regulated in only the extraction group. Conclusion These results demonstrated that Trh, Tnxa and Nnat genes were affected according to the degree of memory in male rats. This study also indicated that S100a9 is a memory-related gene, which is affected by the presence of occlusal support.
| Original language | English |
|---|---|
| Pages (from-to) | 133-141 |
| Number of pages | 9 |
| Journal | Archives of Oral Biology |
| Volume | 59 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2014 |
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Keywords
- Gene expression
- Hippocampus
- Memory and learning
- Nnat
- S100a9
- Tnxa
- Trh
ASJC Scopus subject areas
- Otorhinolaryngology
- Cell Biology
- Dentistry(all)
Cite this
Memory-related gene expression profile of the male rat hippocampus induced by teeth extraction and occlusal support recovery. / Iida, Sachiyo; Hara, Tetsuya; Araki, Daisuke; Ishimine-Kuroda, Chisa; Kurozumi, Akimasa; Sakamoto, Shunichi; Miyazaki, Takako; Minagi, Shougo.
In: Archives of Oral Biology, Vol. 59, No. 2, 02.2014, p. 133-141.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Memory-related gene expression profile of the male rat hippocampus induced by teeth extraction and occlusal support recovery
AU - Iida, Sachiyo
AU - Hara, Tetsuya
AU - Araki, Daisuke
AU - Ishimine-Kuroda, Chisa
AU - Kurozumi, Akimasa
AU - Sakamoto, Shunichi
AU - Miyazaki, Takako
AU - Minagi, Shougo
PY - 2014/2
Y1 - 2014/2
N2 - Objectives The present study aimed to identify the effect of memory-related genes on male rats tested for spatial memory with either molar teeth extraction or its restoration by occlusal support using experimental dentures. Design Memory-related genes were detected from hippocampi of male Wistar rats (exposed to teeth extraction with or without dentures, or no extraction (control)) (7-week old) after behavioural testing (via the radial maze task) using a DNA microarray. The time course of the expression of these genes was evaluated by quantitative real-time polymerase chain reaction (PCR) (on 49-week-old rats). Results In preliminary experiments, to determine which memory genes are affected by spatial memory training, DNA microarray analysis revealed that thyrotropin-releasing hormone (Trh) and tenascin XA (Tnxa) were up-regulated and neuronatin (Nnat) and S100a9 were down-regulated after the maze training. The expression of Tnxa, Nnat and S100a9 of 49-week-old rats (during the time course) via quantitative real-time PCR was consistent with the results of microarrays of the preliminary experiment. Expression of Trh that was evaluated by quantitative real-time PCR did not agree with the results for this gene from the microarray for all groups. Therefore, expression of Trh may have increased in only young, trained rats. The expression of S100a9 prior to the maze task was down-regulated in only the extraction group. Conclusion These results demonstrated that Trh, Tnxa and Nnat genes were affected according to the degree of memory in male rats. This study also indicated that S100a9 is a memory-related gene, which is affected by the presence of occlusal support.
AB - Objectives The present study aimed to identify the effect of memory-related genes on male rats tested for spatial memory with either molar teeth extraction or its restoration by occlusal support using experimental dentures. Design Memory-related genes were detected from hippocampi of male Wistar rats (exposed to teeth extraction with or without dentures, or no extraction (control)) (7-week old) after behavioural testing (via the radial maze task) using a DNA microarray. The time course of the expression of these genes was evaluated by quantitative real-time polymerase chain reaction (PCR) (on 49-week-old rats). Results In preliminary experiments, to determine which memory genes are affected by spatial memory training, DNA microarray analysis revealed that thyrotropin-releasing hormone (Trh) and tenascin XA (Tnxa) were up-regulated and neuronatin (Nnat) and S100a9 were down-regulated after the maze training. The expression of Tnxa, Nnat and S100a9 of 49-week-old rats (during the time course) via quantitative real-time PCR was consistent with the results of microarrays of the preliminary experiment. Expression of Trh that was evaluated by quantitative real-time PCR did not agree with the results for this gene from the microarray for all groups. Therefore, expression of Trh may have increased in only young, trained rats. The expression of S100a9 prior to the maze task was down-regulated in only the extraction group. Conclusion These results demonstrated that Trh, Tnxa and Nnat genes were affected according to the degree of memory in male rats. This study also indicated that S100a9 is a memory-related gene, which is affected by the presence of occlusal support.
KW - Gene expression
KW - Hippocampus
KW - Memory and learning
KW - Nnat
KW - S100a9
KW - Tnxa
KW - Trh
UR - http://www.scopus.com/inward/record.url?scp=84889238192&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84889238192&partnerID=8YFLogxK
U2 - 10.1016/j.archoralbio.2013.10.003
DO - 10.1016/j.archoralbio.2013.10.003
M3 - Article
VL - 59
SP - 133
EP - 141
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
IS - 2
ER -