Abstract
The role of the C-terminal region of the 74-kDa form of l-histidine decarboxylase (HDC) in the targeting to the endoplasmic reticulum (ER) was investigated in COS-7 cells. The deletion of a 10-kDa segment (residues 578-662) of the C-terminal end of HDC, especially a 20 amino acid sequence (residues 588-607), abrogated the targeting to the ER. The C-terminal 10-kDa portion is sufficient to target the green fluorescent protein (GFP) to the ER. The 74-kDa form of HDC synthesized in an in vitro translation system post-translationally associated with the heterogeneous canine microsomal membranes. These results suggest that the C-terminal 10-kDa portion of HDC contains a signal necessary for HDC to be targeted to the ER membrane. Copyright (C) 1998 Federation of European Biochemical Societies.
| Original language | English |
|---|---|
| Pages (from-to) | 44-48 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 437 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Oct 16 1998 |
| Externally published | Yes |
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Keywords
- C-terminal peptide
- COS-7 cell
- Endoplasmic reticulum
- Histidine decarboxylase
- Targeting
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Membrane targeting and binding of the 74-kDa form of mouse L-histidine decarboxylase via its carboxyl-terminal sequence. / Suzuki, Sawako; Tanaka, Satoshi; Nemoto, Ken Ichi; Ichikawa, Atsushi.
In: FEBS Letters, Vol. 437, No. 1-2, 16.10.1998, p. 44-48.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Membrane targeting and binding of the 74-kDa form of mouse L-histidine decarboxylase via its carboxyl-terminal sequence
AU - Suzuki, Sawako
AU - Tanaka, Satoshi
AU - Nemoto, Ken Ichi
AU - Ichikawa, Atsushi
PY - 1998/10/16
Y1 - 1998/10/16
N2 - The role of the C-terminal region of the 74-kDa form of l-histidine decarboxylase (HDC) in the targeting to the endoplasmic reticulum (ER) was investigated in COS-7 cells. The deletion of a 10-kDa segment (residues 578-662) of the C-terminal end of HDC, especially a 20 amino acid sequence (residues 588-607), abrogated the targeting to the ER. The C-terminal 10-kDa portion is sufficient to target the green fluorescent protein (GFP) to the ER. The 74-kDa form of HDC synthesized in an in vitro translation system post-translationally associated with the heterogeneous canine microsomal membranes. These results suggest that the C-terminal 10-kDa portion of HDC contains a signal necessary for HDC to be targeted to the ER membrane. Copyright (C) 1998 Federation of European Biochemical Societies.
AB - The role of the C-terminal region of the 74-kDa form of l-histidine decarboxylase (HDC) in the targeting to the endoplasmic reticulum (ER) was investigated in COS-7 cells. The deletion of a 10-kDa segment (residues 578-662) of the C-terminal end of HDC, especially a 20 amino acid sequence (residues 588-607), abrogated the targeting to the ER. The C-terminal 10-kDa portion is sufficient to target the green fluorescent protein (GFP) to the ER. The 74-kDa form of HDC synthesized in an in vitro translation system post-translationally associated with the heterogeneous canine microsomal membranes. These results suggest that the C-terminal 10-kDa portion of HDC contains a signal necessary for HDC to be targeted to the ER membrane. Copyright (C) 1998 Federation of European Biochemical Societies.
KW - C-terminal peptide
KW - COS-7 cell
KW - Endoplasmic reticulum
KW - Histidine decarboxylase
KW - Targeting
UR - http://www.scopus.com/inward/record.url?scp=0032538531&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032538531&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(98)01195-8
DO - 10.1016/S0014-5793(98)01195-8
M3 - Article
C2 - 9804169
AN - SCOPUS:0032538531
VL - 437
SP - 44
EP - 48
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-2
ER -