Membrane targeting and binding of the 74-kDa form of mouse L-histidine decarboxylase via its carboxyl-terminal sequence

Sawako Suzuki; Satoshi Tanaka; Ken Ichi Nemoto; Atsushi Ichikawa

doi:10.1016/S0014-5793(98)01195-8

Membrane targeting and binding of the 74-kDa form of mouse L-histidine decarboxylase via its carboxyl-terminal sequence

Sawako Suzuki, Satoshi Tanaka, Ken Ichi Nemoto, Atsushi Ichikawa

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The role of the C-terminal region of the 74-kDa form of l-histidine decarboxylase (HDC) in the targeting to the endoplasmic reticulum (ER) was investigated in COS-7 cells. The deletion of a 10-kDa segment (residues 578-662) of the C-terminal end of HDC, especially a 20 amino acid sequence (residues 588-607), abrogated the targeting to the ER. The C-terminal 10-kDa portion is sufficient to target the green fluorescent protein (GFP) to the ER. The 74-kDa form of HDC synthesized in an in vitro translation system post-translationally associated with the heterogeneous canine microsomal membranes. These results suggest that the C-terminal 10-kDa portion of HDC contains a signal necessary for HDC to be targeted to the ER membrane. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	44-48
Number of pages	5
Journal	FEBS Letters
Volume	437
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(98)01195-8
Publication status	Published - Oct 16 1998

Keywords

C-terminal peptide
COS-7 cell
Endoplasmic reticulum
Histidine decarboxylase
Targeting

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Membrane targeting and binding of the 74-kDa form of mouse L-histidine decarboxylase via its carboxyl-terminal sequence",

abstract = "The role of the C-terminal region of the 74-kDa form of l-histidine decarboxylase (HDC) in the targeting to the endoplasmic reticulum (ER) was investigated in COS-7 cells. The deletion of a 10-kDa segment (residues 578-662) of the C-terminal end of HDC, especially a 20 amino acid sequence (residues 588-607), abrogated the targeting to the ER. The C-terminal 10-kDa portion is sufficient to target the green fluorescent protein (GFP) to the ER. The 74-kDa form of HDC synthesized in an in vitro translation system post-translationally associated with the heterogeneous canine microsomal membranes. These results suggest that the C-terminal 10-kDa portion of HDC contains a signal necessary for HDC to be targeted to the ER membrane. Copyright (C) 1998 Federation of European Biochemical Societies.",

keywords = "C-terminal peptide, COS-7 cell, Endoplasmic reticulum, Histidine decarboxylase, Targeting",

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T1 - Membrane targeting and binding of the 74-kDa form of mouse L-histidine decarboxylase via its carboxyl-terminal sequence

AU - Suzuki, Sawako

AU - Tanaka, Satoshi

AU - Nemoto, Ken Ichi

AU - Ichikawa, Atsushi

PY - 1998/10/16

Y1 - 1998/10/16

N2 - The role of the C-terminal region of the 74-kDa form of l-histidine decarboxylase (HDC) in the targeting to the endoplasmic reticulum (ER) was investigated in COS-7 cells. The deletion of a 10-kDa segment (residues 578-662) of the C-terminal end of HDC, especially a 20 amino acid sequence (residues 588-607), abrogated the targeting to the ER. The C-terminal 10-kDa portion is sufficient to target the green fluorescent protein (GFP) to the ER. The 74-kDa form of HDC synthesized in an in vitro translation system post-translationally associated with the heterogeneous canine microsomal membranes. These results suggest that the C-terminal 10-kDa portion of HDC contains a signal necessary for HDC to be targeted to the ER membrane. Copyright (C) 1998 Federation of European Biochemical Societies.

AB - The role of the C-terminal region of the 74-kDa form of l-histidine decarboxylase (HDC) in the targeting to the endoplasmic reticulum (ER) was investigated in COS-7 cells. The deletion of a 10-kDa segment (residues 578-662) of the C-terminal end of HDC, especially a 20 amino acid sequence (residues 588-607), abrogated the targeting to the ER. The C-terminal 10-kDa portion is sufficient to target the green fluorescent protein (GFP) to the ER. The 74-kDa form of HDC synthesized in an in vitro translation system post-translationally associated with the heterogeneous canine microsomal membranes. These results suggest that the C-terminal 10-kDa portion of HDC contains a signal necessary for HDC to be targeted to the ER membrane. Copyright (C) 1998 Federation of European Biochemical Societies.

KW - C-terminal peptide

KW - COS-7 cell

KW - Endoplasmic reticulum

KW - Histidine decarboxylase

KW - Targeting

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Membrane targeting and binding of the 74-kDa form of mouse L-histidine decarboxylase via its carboxyl-terminal sequence

Abstract

Keywords

ASJC Scopus subject areas

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