Membrane targeting and binding of the 74-kDa form of mouse L-histidine decarboxylase via its carboxyl-terminal sequence

Sawako Suzuki, Satoshi Tanaka, Ken Ichi Nemoto, Atsushi Ichikawa

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The role of the C-terminal region of the 74-kDa form of l-histidine decarboxylase (HDC) in the targeting to the endoplasmic reticulum (ER) was investigated in COS-7 cells. The deletion of a 10-kDa segment (residues 578-662) of the C-terminal end of HDC, especially a 20 amino acid sequence (residues 588-607), abrogated the targeting to the ER. The C-terminal 10-kDa portion is sufficient to target the green fluorescent protein (GFP) to the ER. The 74-kDa form of HDC synthesized in an in vitro translation system post-translationally associated with the heterogeneous canine microsomal membranes. These results suggest that the C-terminal 10-kDa portion of HDC contains a signal necessary for HDC to be targeted to the ER membrane. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)44-48
Number of pages5
JournalFEBS Letters
Volume437
Issue number1-2
DOIs
Publication statusPublished - Oct 16 1998
Externally publishedYes

Fingerprint

Histidine Decarboxylase
Histidine
Endoplasmic Reticulum
Membranes
COS Cells
Green Fluorescent Proteins
Canidae
Amino Acid Sequence
Amino Acids

Keywords

  • C-terminal peptide
  • COS-7 cell
  • Endoplasmic reticulum
  • Histidine decarboxylase
  • Targeting

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Membrane targeting and binding of the 74-kDa form of mouse L-histidine decarboxylase via its carboxyl-terminal sequence. / Suzuki, Sawako; Tanaka, Satoshi; Nemoto, Ken Ichi; Ichikawa, Atsushi.

In: FEBS Letters, Vol. 437, No. 1-2, 16.10.1998, p. 44-48.

Research output: Contribution to journalArticle

Suzuki, Sawako ; Tanaka, Satoshi ; Nemoto, Ken Ichi ; Ichikawa, Atsushi. / Membrane targeting and binding of the 74-kDa form of mouse L-histidine decarboxylase via its carboxyl-terminal sequence. In: FEBS Letters. 1998 ; Vol. 437, No. 1-2. pp. 44-48.
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