Membrane orientation of the precursor 74-kDa form of L-histidine decarboxylase

Kazuyuki Furuta, A. Ichikawa, K. Nakayama, S. Tanaka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: We previously demonstrated that, when expressed in COS-7 cells, L-histidine decarboxylase (HDC), which has neither an amino terminal signal sequence nor a hydrophobic membrane anchor, was localized in the endoplasmic reticulum (ER), although its orientation in the membrane remains to be clarified. Methods & Results: Protease digestion and immunofluorescence analyses of the cells, of which plasma membrane was selectively permeabilized, revealed that the amino terminal 50-kDa portion of HDC is hardly accessible to proteases and antibodies added exogenously from the cytosolic side. Green fluorescent protein fused with the carboxyl terminal 20-kDa region of HDC at its carboxyl terminus exhibited the same characteristics as native HDC. Conclusion: These results indicate that HDC is tightly associated with the ER membrane with its carboxyl terminal region exposed on the cytosolic side.

Original languageEnglish
Pages (from-to)185-191
Number of pages7
JournalInflammation Research
Volume55
Issue number5
DOIs
Publication statusPublished - May 2006
Externally publishedYes

Fingerprint

Histidine Decarboxylase
Histidine
Membranes
Endoplasmic Reticulum
Peptide Hydrolases
COS Cells
Cell membranes
Protein Sorting Signals
Green Fluorescent Proteins
Anchors
Fluorescent Antibody Technique
Digestion
Cell Membrane
Antibodies

Keywords

  • Endoplasmic reticulum
  • Green fluorescence protein and protease
  • Histidine decarboxylase
  • Membrane orientation

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)
  • Immunology
  • Cell Biology

Cite this

Membrane orientation of the precursor 74-kDa form of L-histidine decarboxylase. / Furuta, Kazuyuki; Ichikawa, A.; Nakayama, K.; Tanaka, S.

In: Inflammation Research, Vol. 55, No. 5, 05.2006, p. 185-191.

Research output: Contribution to journalArticle

Furuta, Kazuyuki ; Ichikawa, A. ; Nakayama, K. ; Tanaka, S. / Membrane orientation of the precursor 74-kDa form of L-histidine decarboxylase. In: Inflammation Research. 2006 ; Vol. 55, No. 5. pp. 185-191.
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