Membrane interaction of synthetic peptides related to the putative fusogenic region of PH-30α, a protein in sperm-egg fusion

Takuro Niidome, Miyuki Kimura, Tomomi Chiba, Naoya Ohmori, Hisakazu Mihara, Haruhiko Aoyagi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In order to investigate the relationship between structure and function of a putative fusogenic region of PH-30α, a protein active in sperm-egg fusion, two peptides, SFP22 and SFP23, whose sequences correspond to the residues 90-111 and 89-111 of PH-30α, respectively, were chemically synthesized. An analog of SFP23, SFP23AA, which has an Ala-Ala sequence instead of the Pro-Pro sequence in SFP23, was also prepared. The CD study indicated that SFP22 and SFP23 mainly took a β-structure in the presence of DPPC and DPPC/DPPG (3/1) vesicles, while SFP23AA showed an α-helical pattern though the α-helical content calculated was low (25-30%). α-Helical CD curve was observed for these peptides in trifluoroethanol. The membrane- perturbing activity of SFP22 and SFP23 was weaker than that of SFP23AA. On the other hand, the membrane-fusogenic activity of SFP22 and SFP23 to acidic phospholipid bilayers was much stronger than that of SFP23AA. All the peptides caused very weak cell lysis. These results are consistent with the reported speculation [Blobel, C. P. et al. (1992), Nature (London) 356, 248- 252] that residues 90-111 of PH-30α may be the fusogenic region and suggest that the Pro-Pro sequence is one of the important factors for holding the active secondary structure of the fusogenic region of PH-30α in membranes.

Original languageEnglish
Pages (from-to)563-569
Number of pages7
JournalJournal of Peptide Research
Volume49
Issue number6
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Ovum
Spermatozoa
Fusion reactions
Membranes
Peptides
Trifluoroethanol
Proteins
Phospholipids
Fertilins
1,2-dipalmitoylphosphatidylglycerol
3-dodecanoylpropanediol-1-phosphorylcholine

Keywords

  • β-structural peptide
  • Fusogenic peptides
  • Membrane fusion
  • Membrane perturbation
  • PH-30α
  • Sperm-egg fusion

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Membrane interaction of synthetic peptides related to the putative fusogenic region of PH-30α, a protein in sperm-egg fusion. / Niidome, Takuro; Kimura, Miyuki; Chiba, Tomomi; Ohmori, Naoya; Mihara, Hisakazu; Aoyagi, Haruhiko.

In: Journal of Peptide Research, Vol. 49, No. 6, 1997, p. 563-569.

Research output: Contribution to journalArticle

Niidome, T, Kimura, M, Chiba, T, Ohmori, N, Mihara, H & Aoyagi, H 1997, 'Membrane interaction of synthetic peptides related to the putative fusogenic region of PH-30α, a protein in sperm-egg fusion', Journal of Peptide Research, vol. 49, no. 6, pp. 563-569.
Niidome, Takuro ; Kimura, Miyuki ; Chiba, Tomomi ; Ohmori, Naoya ; Mihara, Hisakazu ; Aoyagi, Haruhiko. / Membrane interaction of synthetic peptides related to the putative fusogenic region of PH-30α, a protein in sperm-egg fusion. In: Journal of Peptide Research. 1997 ; Vol. 49, No. 6. pp. 563-569.
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AU - Chiba, Tomomi

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AU - Mihara, Hisakazu

AU - Aoyagi, Haruhiko

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