Membrane fluidity controls redox-regulated cold stress responses in cyanobacteria

Eugene G. Maksimov, Kirill S. Mironov, Marina S. Trofimova, Natalya L. Nechaeva, Daria A. Todorenko, Konstantin E. Klementiev, Georgy V. Tsoraev, Eugene V. Tyutyaev, Anna A. Zorina, Pavel V. Feduraev, Suleyman Allakhverdiev, Vladimir Z. Paschenko, Dmitry A. Los

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Membrane fluidity is the important regulator of cellular responses to changing ambient temperature. Bacteria perceive cold by the transmembrane histidine kinases that sense changes in thickness of the cytoplasmic membrane due to its rigidification. In the cyanobacterium Synechocystis, about a half of cold-responsive genes is controlled by the light-dependent transmembrane histidine kinase Hik33, which also partially controls the responses to osmotic, salt, and oxidative stress. This implies the existence of some universal, but yet unknown signal that triggers adaptive gene expression in response to various stressors. Here we selectively probed the components of photosynthetic machinery and functionally characterized the thermodynamics of cyanobacterial photosynthetic membranes with genetically altered fluidity. We show that the rate of oxidation of the quinone pool (PQ), which interacts with both photosynthetic and respiratory electron transport chains, depends on membrane fluidity. Inhibitor-induced stimulation of redox changes in PQ triggers cold-induced gene expression. Thus, the fluidity-dependent changes in the redox state of PQ may universally trigger cellular responses to stressors that affect membrane properties.

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalPhotosynthesis Research
Volume133
Issue number1-3
DOIs
Publication statusPublished - Sep 1 2017
Externally publishedYes

Fingerprint

Cold-Shock Response
membrane fluidity
Membrane Fluidity
Fluidity
Cyanobacteria
Oxidation-Reduction
histidine kinase
Membranes
Respiratory Transport
Histidine
Gene expression
Synechocystis
Gene Expression
Photosynthetic membranes
gene expression
Phosphotransferases
Osmotic Pressure
electron transport chain
Electron Transport
osmotic stress

Keywords

  • Cyanobacteria
  • Desaturase
  • Fatty acids
  • Fluidity
  • Fluorescence
  • Lipids
  • Membrane
  • Photosystem I
  • Photosystem II
  • Plastoquinone pool
  • Redox regulation

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

Maksimov, E. G., Mironov, K. S., Trofimova, M. S., Nechaeva, N. L., Todorenko, D. A., Klementiev, K. E., ... Los, D. A. (2017). Membrane fluidity controls redox-regulated cold stress responses in cyanobacteria. Photosynthesis Research, 133(1-3), 215-223. https://doi.org/10.1007/s11120-017-0337-3

Membrane fluidity controls redox-regulated cold stress responses in cyanobacteria. / Maksimov, Eugene G.; Mironov, Kirill S.; Trofimova, Marina S.; Nechaeva, Natalya L.; Todorenko, Daria A.; Klementiev, Konstantin E.; Tsoraev, Georgy V.; Tyutyaev, Eugene V.; Zorina, Anna A.; Feduraev, Pavel V.; Allakhverdiev, Suleyman; Paschenko, Vladimir Z.; Los, Dmitry A.

In: Photosynthesis Research, Vol. 133, No. 1-3, 01.09.2017, p. 215-223.

Research output: Contribution to journalArticle

Maksimov, EG, Mironov, KS, Trofimova, MS, Nechaeva, NL, Todorenko, DA, Klementiev, KE, Tsoraev, GV, Tyutyaev, EV, Zorina, AA, Feduraev, PV, Allakhverdiev, S, Paschenko, VZ & Los, DA 2017, 'Membrane fluidity controls redox-regulated cold stress responses in cyanobacteria', Photosynthesis Research, vol. 133, no. 1-3, pp. 215-223. https://doi.org/10.1007/s11120-017-0337-3
Maksimov EG, Mironov KS, Trofimova MS, Nechaeva NL, Todorenko DA, Klementiev KE et al. Membrane fluidity controls redox-regulated cold stress responses in cyanobacteria. Photosynthesis Research. 2017 Sep 1;133(1-3):215-223. https://doi.org/10.1007/s11120-017-0337-3
Maksimov, Eugene G. ; Mironov, Kirill S. ; Trofimova, Marina S. ; Nechaeva, Natalya L. ; Todorenko, Daria A. ; Klementiev, Konstantin E. ; Tsoraev, Georgy V. ; Tyutyaev, Eugene V. ; Zorina, Anna A. ; Feduraev, Pavel V. ; Allakhverdiev, Suleyman ; Paschenko, Vladimir Z. ; Los, Dmitry A. / Membrane fluidity controls redox-regulated cold stress responses in cyanobacteria. In: Photosynthesis Research. 2017 ; Vol. 133, No. 1-3. pp. 215-223.
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