Melatonin counteracts BMP-6 regulation of steroidogenesis by rat granulosa cells

Eri Nakamura, Fumio Otsuka, Tomohiro Terasaka, Kenichi Inagaki, Takeshi Hosoya, Naoko Tsukamoto-Yamauchi, Kishio Toma, Hirofumi Makino

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The ovarian bone morphogenetic protein (BMP) system is a physiological inhibitor of luteinization in growing ovarian follicles. BMP-6, which is expressed in oocytes and granulosa cells of healthy follicles, specifically inhibits FSH actions by suppressing adenylate cyclase activity. In the present study, we studied the role of melatonin in ovarian steroidogenesis using rat primary granulosa cells of immature female rat ovaries by focusing on the interaction with BMP-6 activity. Treatment with melatonin had no direct effect on FSH-induced progesterone or estradiol production by granulosa cells, and the results were not affected by the presence of co-cultured oocytes. In addition, synthesis of cAMP by granulosa cells was not significantly altered by melatonin treatment. To elucidate the interaction between activities of melatonin and BMPs, the effect of melatonin treatment on suppression of progesterone synthesis by BMP-6 was investigated. Interestingly, the inhibitory effect of BMP-6 on FSH-induced progesterone production was impaired by co-treatment with melatonin. Granulosa cells express higher levels of MT1 than MT2, and BMP-6 had no significant effect on MT1 expression in granulosa cells. However, BMP-6-induced Smad1/5/8 phosphorylation and Id-1 transcription were suppressed by melatonin, suggesting that melatonin has an inhibitory effect on BMP receptor signaling in granulosa cells. Although the expression levels of ALK-2, -6, ActRII and BMPRII were not affected by melatonin, inhibitory Smad6, but not Smad7, expression was upregulated by melatonin. Thus, melatonin plays a role in the regulation of BMP-6 signal intensity for controlling progesterone production in the ovary. These findings suggest that the effect of melatonin on maintenance of ovarian function is, at least in part, due to the regulation of endogenous BMP activity in granulosa cells.

Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume143
DOIs
Publication statusPublished - 2014

Fingerprint

Bone Morphogenetic Protein 6
Granulosa Cells
Melatonin
Rats
Progesterone
Bone Morphogenetic Proteins
Oocytes
Ovary
Bone Morphogenetic Protein Receptors
Luteinization
Phosphorylation
Ovarian Follicle
Transcription
Adenylyl Cyclases

Keywords

  • Bone morphogenetic protein (BMP)
  • Follicle-stimulating hormone (FSH)
  • Granulosa cells
  • Melatonin
  • Oocyte
  • Steroidogenesis

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Cell Biology
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Molecular Medicine

Cite this

Melatonin counteracts BMP-6 regulation of steroidogenesis by rat granulosa cells. / Nakamura, Eri; Otsuka, Fumio; Terasaka, Tomohiro; Inagaki, Kenichi; Hosoya, Takeshi; Tsukamoto-Yamauchi, Naoko; Toma, Kishio; Makino, Hirofumi.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 143, 2014, p. 233-239.

Research output: Contribution to journalArticle

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AU - Nakamura, Eri

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