Melanization in albino mice transformed by introducing cloned mouse tyrosinase gene

Satoshi Tanaka, Hiroaki Yamamoto, Sakae Takeuchi, Takuji Takeuchi

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

We introduced a mouse tyrosinase minigene, mg-Tyrs-J, in which the authentic genomic 5′ non-coding flanking sequence was fused to a mouse tyrosinase cDNA, into fertilized eggs of albino mice. Of the 25 animals that developed from the injected eggs, four mice exhibited pigmented hair and eyes. Histological analysis of the transgenic mice revealed that the melanogenesis was restricted to hair bulbs and eyes. These results suggest that this minigene encodes active tyrosinase protein and that its 5′ flanking region contains the sequences regulating expression of mouse tyrosinase gene. This is the first report of a successful expression of tyrosinase gene and of pigment production in transgenic mice.

Original languageEnglish
Pages (from-to)223-227
Number of pages5
JournalDevelopment
Volume108
Issue number2
Publication statusPublished - Feb 1990
Externally publishedYes

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Monophenol Monooxygenase
Genes
Hair
Transgenic Mice
Zygote
5' Flanking Region
Eggs
Complementary DNA
Gene Expression
Proteins

Keywords

  • Albino
  • Melanization
  • Transgenic mouse
  • Tyrosinase gene

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Melanization in albino mice transformed by introducing cloned mouse tyrosinase gene. / Tanaka, Satoshi; Yamamoto, Hiroaki; Takeuchi, Sakae; Takeuchi, Takuji.

In: Development, Vol. 108, No. 2, 02.1990, p. 223-227.

Research output: Contribution to journalArticle

Tanaka, S, Yamamoto, H, Takeuchi, S & Takeuchi, T 1990, 'Melanization in albino mice transformed by introducing cloned mouse tyrosinase gene', Development, vol. 108, no. 2, pp. 223-227.
Tanaka, Satoshi ; Yamamoto, Hiroaki ; Takeuchi, Sakae ; Takeuchi, Takuji. / Melanization in albino mice transformed by introducing cloned mouse tyrosinase gene. In: Development. 1990 ; Vol. 108, No. 2. pp. 223-227.
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