Melanin production in cultured albino melanocytes transfected with mouse tyrosinase cDNA

Hiroaki Yamamoto, Sakae Takeuchi, Toshiyuki Kudo, Chikara Sato, Takuji Takeuchi

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

An attempt was made in the present study to express mouse tyrosinase cDNAs fused with the authentic genomic 5′ non-coding flanking sequence in cultured albino melanocytes. One of the cDNA sequences, which expressed successfully and produced melanin pigments, was analyzed with respect to deduced amino acid sequence. Sequencing of the tyrosinase genomic gene revealed the existence of several sets of a characteristic structure which consists of a chain of two successive stem structures, CCAAT-homology and TATA box at its 5′ non-coding region. It seems possible that this region represents the regulatory element of the tyrosinase gene. Unusually long GA cluster at 5′ upstream region was also found.

Original languageEnglish
Pages (from-to)121-135
Number of pages15
JournalThe Japanese Journal of Genetics
Volume64
Issue number2
DOIs
Publication statusPublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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