Meiotic inheritance of a fungal supernumerary chromosome and its effect on sexual fertility in Nectria haematococca

Hamid S. Garmaroodi, Masatoki Taga

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

PDA1-conditionally dispensable chromosome (CDC) of Nectria haematococca MP VI has long served as a model of supernumerary chromosomes in plant pathogenic fungi because of pathogenicity-related genes located on it. In our previous study, we showed the dosage effects of PDA1-CDC on pathogenicity and homoserine utilization by exploiting tagged PDA1-CDC with a marker gene. CDC content of mating partners and progenies analyzed by PCR, PFGE combined with Southern analysis and chromosome painting via FISH. In this study, we analyzed mode of meiotic inheritance of PDA1-CDC in several mating patterns with regard to CDC content and found a correlation between CDC content of parental strains with fertility of crosses. The results showed non-Mendelian inheritance of this chromosome followed by duplication or loss of the CDC in haploid genome through meiosis that probably were due to premature centromere division, not by nondisjunction as reported for the supernumerary chromosomes in other species. Correlation of CDC with fertility is the first time to be examined in fungi in this study.

Original languageEnglish
Article number603
Pages (from-to)929-939
Number of pages11
JournalFungal Biology
Volume119
Issue number10
DOIs
Publication statusPublished - Oct 1 2015

Fingerprint

Fungal Chromosomes
Nectria
Fusarium eumartii
Fertility
fertility
chromosome
inheritance (genetics)
Chromosomes
chromosomes
Virulence
pathogenicity
Fungi
Plant Chromosomes
B chromosomes
effect
Chromosome Duplication
Chromosome Painting
Homoserine
homoserine
Centromere

Keywords

  • Conditionally dispensable chromosome (CDC)
  • FISH
  • Nondisjunction

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Genetics
  • Infectious Diseases

Cite this

Meiotic inheritance of a fungal supernumerary chromosome and its effect on sexual fertility in Nectria haematococca. / Garmaroodi, Hamid S.; Taga, Masatoki.

In: Fungal Biology, Vol. 119, No. 10, 603, 01.10.2015, p. 929-939.

Research output: Contribution to journalArticle

Garmaroodi, Hamid S. ; Taga, Masatoki. / Meiotic inheritance of a fungal supernumerary chromosome and its effect on sexual fertility in Nectria haematococca. In: Fungal Biology. 2015 ; Vol. 119, No. 10. pp. 929-939.
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