Megakaryoblastic leukemia cell line MOLM-16 derived from minimally differentiated acute leukemia with myeloid/NK precursor phenotype

Yoshinobu Matsuo; Hans G. Drexler; Kinuyo Kaneda; Kensuke Kojima; Yuji Ohtsuki; Masamichi Hara; Masaki Yasukawa; Mitsune Tanimoto; Kunzo Orita

doi:10.1016/S0145-2126(02)00081-4

Megakaryoblastic leukemia cell line MOLM-16 derived from minimally differentiated acute leukemia with myeloid/NK precursor phenotype

Yoshinobu Matsuo, Hans G. Drexler, Kinuyo Kaneda, Kensuke Kojima, Yuji Ohtsuki, Masamichi Hara, Masaki Yasukawa, Mitsune Tanimoto, Kunzo Orita

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The megakaryoblastic leukemia cell line MOLM-16 was established at relapse from the peripheral blood of a 77-year-old Japanese woman with minimally differentiated acute myeloid leukemia (AML-M0). Immunophenotyping of the fresh leukemic cells revealed a myeloid/NK precursor phenotype being positive for CD7, CD13, CD33, CD34, and CD56. In addition, megakaryocyte-associated antigens CD41 and CD61 were found to be positive. The established cell line designated MOLM-16 was proliferatively responsive to the treatment with various cytokines including EPO, GM-CSF, IL-3, PIXY-321, and TPO. MOLM-16 revealed characteristics of the megakaryocytic lineage in terms of immunophenotyping being positive for CD9, CD31, CD36, CD41, CD61, CD62P, CD63, CD110, CD151, thrombospondin, von Willebrand factor (vWf), and fibrinogen. Electron microscopic analysis showed positivity for ultrastructural platelet peroxidase in the nuclear envelope. The karyotype analysis of MOLM-16 revealed various numerical and structural abnormalities including t(6;8)(q21;q24.3), t(9;18)(q13;q21) and marker chromosomes. The extensive immunological, cytogenetic and functional characterization of MOLM-16 suggests that this cell line may represent a scientifically significant in vitro model which could facilitate the evaluation of megakaryocytic differentiation.

Original language	English
Pages (from-to)	165-171
Number of pages	7
Journal	Leukemia Research
Volume	27
Issue number	2
DOIs	https://doi.org/10.1016/S0145-2126(02)00081-4
Publication status	Published - Feb 1 2003

Keywords

Acute minimally differentiated leukemia
Megakaryoblastic cell line
Myeloid/NK precursor leukemia

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

Access to Document

10.1016/S0145-2126(02)00081-4

Fingerprint Dive into the research topics of 'Megakaryoblastic leukemia cell line MOLM-16 derived from minimally differentiated acute leukemia with myeloid/NK precursor phenotype'. Together they form a unique fingerprint.

Cite this

Megakaryoblastic leukemia cell line MOLM-16 derived from minimally differentiated acute leukemia with myeloid/NK precursor phenotype. / Matsuo, Yoshinobu; Drexler, Hans G.; Kaneda, Kinuyo; Kojima, Kensuke; Ohtsuki, Yuji; Hara, Masamichi; Yasukawa, Masaki; Tanimoto, Mitsune; Orita, Kunzo.

In: Leukemia Research, Vol. 27, No. 2, 01.02.2003, p. 165-171.

Research output: Contribution to journal › Article › peer-review

@article{4c1af6e89e2a42d6a5b727ca9d489e1c,

title = "Megakaryoblastic leukemia cell line MOLM-16 derived from minimally differentiated acute leukemia with myeloid/NK precursor phenotype",

abstract = "The megakaryoblastic leukemia cell line MOLM-16 was established at relapse from the peripheral blood of a 77-year-old Japanese woman with minimally differentiated acute myeloid leukemia (AML-M0). Immunophenotyping of the fresh leukemic cells revealed a myeloid/NK precursor phenotype being positive for CD7, CD13, CD33, CD34, and CD56. In addition, megakaryocyte-associated antigens CD41 and CD61 were found to be positive. The established cell line designated MOLM-16 was proliferatively responsive to the treatment with various cytokines including EPO, GM-CSF, IL-3, PIXY-321, and TPO. MOLM-16 revealed characteristics of the megakaryocytic lineage in terms of immunophenotyping being positive for CD9, CD31, CD36, CD41, CD61, CD62P, CD63, CD110, CD151, thrombospondin, von Willebrand factor (vWf), and fibrinogen. Electron microscopic analysis showed positivity for ultrastructural platelet peroxidase in the nuclear envelope. The karyotype analysis of MOLM-16 revealed various numerical and structural abnormalities including t(6;8)(q21;q24.3), t(9;18)(q13;q21) and marker chromosomes. The extensive immunological, cytogenetic and functional characterization of MOLM-16 suggests that this cell line may represent a scientifically significant in vitro model which could facilitate the evaluation of megakaryocytic differentiation.",

keywords = "Acute minimally differentiated leukemia, Megakaryoblastic cell line, Myeloid/NK precursor leukemia",

author = "Yoshinobu Matsuo and Drexler, {Hans G.} and Kinuyo Kaneda and Kensuke Kojima and Yuji Ohtsuki and Masamichi Hara and Masaki Yasukawa and Mitsune Tanimoto and Kunzo Orita",

year = "2003",

month = feb,

day = "1",

doi = "10.1016/S0145-2126(02)00081-4",

language = "English",

volume = "27",

pages = "165--171",

journal = "Leukemia Research",

issn = "0145-2126",

publisher = "Elsevier Limited",

number = "2",

}

TY - JOUR

T1 - Megakaryoblastic leukemia cell line MOLM-16 derived from minimally differentiated acute leukemia with myeloid/NK precursor phenotype

AU - Matsuo, Yoshinobu

AU - Drexler, Hans G.

AU - Kaneda, Kinuyo

AU - Kojima, Kensuke

AU - Ohtsuki, Yuji

AU - Hara, Masamichi

AU - Yasukawa, Masaki

AU - Tanimoto, Mitsune

AU - Orita, Kunzo

PY - 2003/2/1

Y1 - 2003/2/1

N2 - The megakaryoblastic leukemia cell line MOLM-16 was established at relapse from the peripheral blood of a 77-year-old Japanese woman with minimally differentiated acute myeloid leukemia (AML-M0). Immunophenotyping of the fresh leukemic cells revealed a myeloid/NK precursor phenotype being positive for CD7, CD13, CD33, CD34, and CD56. In addition, megakaryocyte-associated antigens CD41 and CD61 were found to be positive. The established cell line designated MOLM-16 was proliferatively responsive to the treatment with various cytokines including EPO, GM-CSF, IL-3, PIXY-321, and TPO. MOLM-16 revealed characteristics of the megakaryocytic lineage in terms of immunophenotyping being positive for CD9, CD31, CD36, CD41, CD61, CD62P, CD63, CD110, CD151, thrombospondin, von Willebrand factor (vWf), and fibrinogen. Electron microscopic analysis showed positivity for ultrastructural platelet peroxidase in the nuclear envelope. The karyotype analysis of MOLM-16 revealed various numerical and structural abnormalities including t(6;8)(q21;q24.3), t(9;18)(q13;q21) and marker chromosomes. The extensive immunological, cytogenetic and functional characterization of MOLM-16 suggests that this cell line may represent a scientifically significant in vitro model which could facilitate the evaluation of megakaryocytic differentiation.

AB - The megakaryoblastic leukemia cell line MOLM-16 was established at relapse from the peripheral blood of a 77-year-old Japanese woman with minimally differentiated acute myeloid leukemia (AML-M0). Immunophenotyping of the fresh leukemic cells revealed a myeloid/NK precursor phenotype being positive for CD7, CD13, CD33, CD34, and CD56. In addition, megakaryocyte-associated antigens CD41 and CD61 were found to be positive. The established cell line designated MOLM-16 was proliferatively responsive to the treatment with various cytokines including EPO, GM-CSF, IL-3, PIXY-321, and TPO. MOLM-16 revealed characteristics of the megakaryocytic lineage in terms of immunophenotyping being positive for CD9, CD31, CD36, CD41, CD61, CD62P, CD63, CD110, CD151, thrombospondin, von Willebrand factor (vWf), and fibrinogen. Electron microscopic analysis showed positivity for ultrastructural platelet peroxidase in the nuclear envelope. The karyotype analysis of MOLM-16 revealed various numerical and structural abnormalities including t(6;8)(q21;q24.3), t(9;18)(q13;q21) and marker chromosomes. The extensive immunological, cytogenetic and functional characterization of MOLM-16 suggests that this cell line may represent a scientifically significant in vitro model which could facilitate the evaluation of megakaryocytic differentiation.

KW - Acute minimally differentiated leukemia

KW - Megakaryoblastic cell line

KW - Myeloid/NK precursor leukemia

UR - http://www.scopus.com/inward/record.url?scp=0037297064&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037297064&partnerID=8YFLogxK

U2 - 10.1016/S0145-2126(02)00081-4

DO - 10.1016/S0145-2126(02)00081-4

M3 - Article

C2 - 12526922

AN - SCOPUS:0037297064

VL - 27

SP - 165

EP - 171

JO - Leukemia Research

JF - Leukemia Research

SN - 0145-2126

IS - 2

ER -

Megakaryoblastic leukemia cell line MOLM-16 derived from minimally differentiated acute leukemia with myeloid/NK precursor phenotype

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Cite this