Megabirnavirus structure reveals a putative 120-subunit capsid formed by asymmetrical dimers with distinctive large protrusions

Naoyuki Miyazaki, Lakha Salaipeth, Satoko Kanematsu, Kenji Iwasaki, Nobuhiro Suzuki

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)

    Abstract

    Rosellinia necatrix megabirnavirus 1 (RnMBV1) W779 is a bi-segmented dsRNA virus and a strain of the type species Rosellinia necatrix megabirnavirus 1 of the family Megabirnaviridae. RnMBV1 causes severe reduction of both mycelial growth of Rosellinia necatrix in synthetic medium and fungal virulence to plant hosts, and thus has strong potential for virocontrol (biological control using viruses) of white rot. The structure of RnMBV1 was examined by cryo-electron microscopy and three-dimensional reconstruction at 15.7 Å resolution. The diameter of the RnMBV1 capsid was 520 Å, and the capsid was composed of 60 asymmetrical dimers in the T=1 (so-called T=2) lattice that is well conserved among dsRNA viruses. However, RnMBV1 has putatively 120 large protrusions with a width of ∼45 Å and a height of ∼50 Å on the virus surface, making it distinguishable from the other dsRNA viruses.

    Original languageEnglish
    Pages (from-to)2435-2441
    Number of pages7
    JournalJournal of General Virology
    Volume96
    Issue number8
    DOIs
    Publication statusPublished - Aug 1 2015

    ASJC Scopus subject areas

    • Virology

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